Tobacco inbred and hybrid plants and tobacco products made thereof

ABSTRACT

The present disclosure provides tobacco inbred plants Banket A1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley 21 SRC, and CMS Burley 21 SRC. The present disclosure also provides parts of such plants and cured tobacco and tobacco products made from those parts. The present disclosure also includes progenies of the provided plants including hybrids.

CROSS REFERENCE TO RELATED APPLICATIONS

This application claims benefit under 35 U.S.C. §119(e) of U.S.provisional application Ser. No. 61/946,978, filed Mar. 3, 2014, U.S.provisional application Ser. No. 61/946,981, filed Mar. 3, 2014, U.S.provisional application Ser. No. 61/946,983, filed Mar. 3, 2014, U.S.provisional application Ser. No. 61/946,988, filed Mar. 3, 2014, U.S.provisional application Ser. No. 61/978,253, filed Apr. 11, 2014, U.S.provisional application Ser. No. 61/978,267, filed Apr. 11, 2014, U.S.provisional application Ser. No. 61/978,305, filed Apr. 11, 2014, andU.S. provisional application Ser. No. 61/978,312, filed Apr. 11, 2014,all of which are incorporated herein by reference in their entireties.

INCORPORATION OF SEQUENCE LISTING

The Sequence Listing is hereby incorporated by reference in itsentirety, including the file named P34119US02.txt, which is 38,330 bytesin size and was generated on Feb. 23, 2015, which is likewise hereinincorporated by reference in its entirety.

FIELD

The present disclosure provides tobacco inbred plants Banket A1 SRC, CMSBanket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002 SRC, CMS NC 2002SRC, Burley 21 SRC, and CMS Burley 21 SRC. The present disclosure alsoprovides parts of such plants and cured tobacco and tobacco productsmade from those parts. The present disclosure also includes progenies ofthe provided plants including hybrids.

BACKGROUND

Tobacco (Nicotiana tabacum L.) is an important commercial crop in theUnited States as well as in other countries. In tobacco plants,N-demethylation of nicotine results in nornicotine, a secondary alkaloidknown to be a precursor for formation of N-Nitrosonornicotine (“NNN”) incured leaves. NNN is an undesired component of cured leaves.

The predominant alkaloid found in commercial tobacco varieties isnicotine, typically accounting for 90-95% of the total alkaloid pool.The remaining alkaloid fraction is comprised primarily of threeadditional pyridine alkaloids: nornicotine, anabasine, and anatabine.Nornicotine is generated directly from nicotine through the activity ofthe enzyme nicotine N-demethylase. Nornicotine usually represents lessthan 5% of the total pyridine alkaloid pool, but through a processtermed “conversion,” tobacco plants that initially produce very lowamounts of nornicotine give rise to progeny that metabolically “convert”a large percentage of leaf nicotine to nornicotine. In tobacco plantsthat have genetically converted (termed “converters”), the greatmajority of nornicotine production occurs during the senescence andcuring of the mature leaf (Wernsman and Matzinger (1968), Tob. Sci.,12:226-228). Burley tobaccos are particularly prone to geneticconversion, with rates as high as 20% per generation observed in somecultivars.

During the curing and processing of the tobacco leaf, a portion of thenornicotine is metabolized to the compound NNN, a tobacco-specificnitrosamine (TSNA) that has been asserted to be carcinogenic inlaboratory animals (Hecht and Hoffmann (1990), Cancer Surveys,8:273-294; Hoffmann et al. (1994), J. Toxicol. Environ. Health, 41:1-52;Hecht (1998), Chem. Res. Toxicol., 11:559-603). In flue-cured tobaccos,TSNAs are found to be predominantly formed through the reaction ofalkaloids with the minute amounts of nitrogen oxides present incombustion gases formed by the direct-fired heating systems found intraditional curing barns (Peele and Gentry (1999), “Formation ofTobacco-specific Nitrosamines in Flue-cured Tobacco,” CORESTA Meeting,Agro-Phyto Groups, Suzhou, China). Retrofitting these curing barns withheat-exchangers virtually eliminated the mixing of combustion gases withthe curing air and dramatically reduced the formation of TSNAs intobaccos cured in this manner (Boyette and Hamm (2001), Rec. Adv. Tob.Sci., 27:17-22.). In contrast, in the air-cured Burley tobaccos, TSNAformation proceeds primarily through reaction of tobacco alkaloids withnitrite, a process catalyzed by leaf-borne microbes (Bush et al. (2001),Rec. Adv. Tob. Sci., 27:23-46). Thus far, attempts to reduce TSNAsthrough modification of curing conditions while maintaining acceptablequality standards have not proven to be successful for the air-curedtobaccos.

SUMMARY

In an aspect, the present disclosure includes a seed of tobacco cultivarBanket A1 SRC, a representative sample seed of the cultivar having beendeposited with the American Type Culture Collection (ATCC) under ATCCAccession No. PTA-121043.

In another aspect, the present disclosure includes a tobacco plant, orpart thereof, produced by growing the seed of tobacco cultivar Banket A1SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121043.

In a further aspect, the present disclosure includes a harvested leaf,or part thereof, of a tobacco plant, or part thereof, produced bygrowing the seed of tobacco cultivar Banket A1 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121043.

In an aspect, the present disclosure includes a harvested leaf, or partthereof, of a tobacco plant, produced by growing the seed of tobaccocultivar Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121043,where the leaf has a reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN).

In an aspect, the present disclosure includes a harvested leaf, or partthereof, produced by growing the seed of tobacco cultivar Banket A1 SRC,a representative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121043, where the leaf has areduced amount of nornicotine and/or N′-nitrosonornicotine (NNN), wherethe reduced amount of nornicotine and/or N′-nitrosonomicotine (NNN) isreduced in a smoke stream produced from the leaf.

In a further aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar Banket A1 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121043, where the product is selected from the group consistingof pipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof.

In an aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar Banket A1 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121043, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco.

In another aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar Banket A1 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121043, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco, and wherethe product has a reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN).

In a further aspect, the present disclosure includes a second tobaccoproduct prepared or produced from a first tobacco product prepared froma tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar Banket A1 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121043, where first product is selected from the group consisting ofpipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof, andthe second tobacco product is selected from the group consisting of acigarillo, a non-ventilated recess filter cigarette, a vented recessfilter cigarette, a cigar, snuff, and chewing tobacco. In some aspects,the first and second product comprises a reduced amount of nornicotineand/or N′-nitrosonornicotine (NNN).

In an aspect, the present disclosure includes a part of a tobacco plant,produced by growing the seed of tobacco cultivar Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121043, where the part is selectedfrom the group consisting of leaf, pollen, ovule, embryo, cotyledon,hypocotyl, meristematic cell, protoplast, root, root tip, pistil,anther, flower, shoot, stem, pod and petiole.

In another aspect, the present disclosure includes a tissue cultureproduced from a protoplast or cell from a tobacco plant, or partthereof, produced by growing the seed of tobacco cultivar Banket A1 SRC,a representative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121043, where the cell orprotoplast of the tissue culture is produced from a plant part selectedfrom the group consisting of a leaf, pollen, embryo, cotyledon,hypocotyl, meristematic cell, root, root tip, pistil, anther, flower,shoot, stem, pod, and petiole.

In an aspect, the present disclosure includes a tobacco plantregenerated from a tissue culture produced from a protoplast or cellfrom a tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar Banket A1 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121043, where the cell or protoplast of the tissue culture isproduced from a plant part selected from the group consisting of a leaf,pollen, embryo, cotyledon, hypocotyl, meristematic cell, root, root tip,pistil, anther, flower, shoot, stem, pod, and petiole where theregenerated plant has all, or essentially all of the morphological andphysiological characteristics of cultivar Banket A1 SRC.

In an aspect, the present disclosure includes an F₁ progeny plant oftobacco cultivar Banket A1 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121043.

In another aspect, the present disclosure includes an F₁ progeny plantof tobacco cultivar Banket A1 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121043, where the F₁ plant is cytoplasmic male sterile (CMS).

In another aspect, the present disclosure includes an F₁ progeny seedproduced by crossing two tobacco plants and harvesting the resultanttobacco seed, where at least one tobacco plant is a tobacco plantproduced by growing the seed of tobacco cultivar Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121043, where at least one tobaccoplant is cytoplasmic male sterile (CMS).

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivarBanket A1 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121043.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivarBanket A1 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121043, where theplant of tobacco cultivar Banket A1 SRC is the male parent.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivarBanket A1 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121043, where theplant of tobacco cultivar Banket A1 SRC is the female parent.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivarBanket A1 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121043, where atleast one tobacco plant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure also includes a container of F₁progeny seeds produced by crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is a tobaccoplant produced by growing the seed of tobacco cultivar Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121043, where at least one tobaccoplant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure includes an F₁ progeny plantproduced by growing a seed produced by crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivarBanket A1 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121043, and whereat least one tobacco plant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is a tobacco plant produced by growing the seed oftobacco cultivar Banket A1 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121043, where at least one tobacco plant is cytoplasmic male sterile(CMS).

In another aspect, the present disclosure further includes a harvestedleaf of an F₁ progeny plant having a reduced amount of nornicotineand/or N′-nitrosonornicotine (NNN) produced by growing a seed producedby a method of comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is a tobaccoplant produced by growing the seed of tobacco cultivar Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121043, where at least one tobaccoplant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant having a reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN) in a smoke stream produced from the leaf,where the plant is produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is a tobacco plant produced by growing the seed oftobacco cultivar Banket A1 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121043, where at least one tobacco plant is cytoplasmic male sterile(CMS).

In an aspect, the present disclosure includes a tobacco product preparedfrom an F₁ progeny tobacco plant, or part thereof, where the plant orpart thereof is produced by growing an F₁ progeny seed produced by amethod comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is produced bygrowing a seed of tobacco cultivar Banket A1 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121043, where at least one tobacco plant iscytoplasmic male sterile, and where the tobacco product is selected fromthe group consisting of pipe tobacco, cigar tobacco, cigarette tobacco,chewing tobacco, leaf tobacco, shredded tobacco, cut tobacco, and anycombination thereof.

In an aspect, the present disclosure further includes a tobacco productprepared from an F₁ progeny tobacco plant, or part thereof, where theplant or part thereof is produced by growing an F₁ progeny seed producedby a method comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is produced bygrowing a seed of tobacco cultivar Banket A1 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121043, where at least one tobacco plant iscytoplasmic male sterile, and where the tobacco product is selected fromthe group consisting of a cigarillo, a non-ventilated recess filtercigarette, a vented recess filter cigarette, a cigar, snuff, and chewingtobacco.

In another aspect, the present disclosure includes a seed of tobaccocultivar CMS Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121054.

In an aspect, the present disclosure includes a tobacco plant, or partthereof, produced by growing the seed of tobacco cultivar CMS Banket A1SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121054.

In another aspect, the present disclosure includes a harvested leaf, orpart thereof, of a tobacco plant, or part thereof, produced by growingthe seed of tobacco cultivar CMS Banket A1 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121054.

In an aspect, the present disclosure includes a harvested leaf, or partthereof, of a tobacco plant, produced by growing the seed of tobaccocultivar CMS Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121054,where the leaf has a reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN).

In another aspect, the present disclosure includes a harvested leaf, orpart thereof, produced by growing the seed of tobacco cultivar CMSBanket A1 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121054, where theleaf has a reduced amount of nornicotine and/or N′-nitrosonornicotine(NNN), where the reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN) is reduced in a smoke stream produced fromthe leaf.

In a further aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar CMS Banket A1 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121054, where the product is selected from the group consistingof pipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof.

In an aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar CMS Banket A1 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121054, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco.

In another aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar CMS Banket A1 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121054, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco where theproduct has a reduced amount of nornicotine and/or N′-nitrosonornicotine(NNN).

In a further aspect, the present disclosure includes a second tobaccoproduct prepared or produced from a first tobacco product prepared froma tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar CMS Banket A1 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121054, where first product is selected from the group consisting ofpipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof, andthe second tobacco product is selected from the group consisting of acigarillo, a non-ventilated recess filter cigarette, a vented recessfilter cigarette, a cigar, snuff, and chewing tobacco. In some aspects,the first and/or the second product comprises a reduced amount ofnornicotine and/or N′-nitrosonornicotine (NNN).

In an aspect, the present disclosure includes a part of a tobacco plant,produced by growing the seed of tobacco cultivar CMS Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121054, where the part is selectedfrom the group consisting of leaf, pollen, ovule, embryo, cotyledon,hypocotyl, meristematic cell, protoplast, root, root tip, pistil,anther, flower, shoot, stem, pod, and petiole.

In another aspect, the present disclosure includes a tissue cultureproduced from a protoplast or cell from a tobacco plant, or partthereof, produced by growing the seed of tobacco cultivar CMS Banket A1SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121054, where the cell orprotoplast of the tissue culture is produced from a plant part selectedfrom the group consisting of a leaf, pollen, embryo, cotyledon,hypocotyl, meristematic cell, root, root tip, pistil, anther, flower,shoot, stem, pod, and petiole.

In an aspect, the present disclosure includes a tobacco plantregenerated from a tissue culture produced from a protoplast or cellfrom a tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar CMS Banket A1 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121054, where the cell or protoplast of the tissue culture can beproduced from a plant part selected from the group consisting of a leaf,pollen, embryo, cotyledon, hypocotyl, meristematic cell, root, root tip,pistil, anther, flower, shoot, stem, pod, and petiole where theregenerated plant has all, or essentially all of the morphological andphysiological characteristics of cultivar CMS Banket A1 SRC.

In an aspect, the present disclosure includes an F₁ progeny plant oftobacco cultivar CMS Banket A1 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121054.

In another aspect, the present disclosure includes an F₁ progeny plantof tobacco cultivar CMS Banket A1 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121054, where the F₁ plant is cytoplasmic male sterile (CMS).

In another aspect, the present disclosure includes an F₁ progeny seedproduced by crossing two tobacco plants and harvesting the resultanttobacco seed, where at least one tobacco plant is cytoplasmic malesterile (CMS), where the cytoplasmic male sterile plant is a plant oftobacco cultivar CMS Banket A1 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121054.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar CMSBanket A1 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121054.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar CMSBanket A1 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121054, where theplant of tobacco cultivar CMS Banket A1 SRC is the female parent.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis cytoplasmic male sterile (CMS), where the cytoplasmic male sterileplant is a plant of tobacco cultivar CMS Banket A1 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121054.

In an aspect, the present disclosure also includes a container of F₁progeny seeds produced by crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is cytoplasmicmale sterile (CMS), where the cytoplasmic male sterile plant is atobacco plant produced by growing the seed of tobacco cultivar CMSBanket A1 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121054.

In an aspect, the present disclosure includes an F₁ progeny plantproduced by growing a seed produced by crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis cytoplasmic male sterile (CMS), where the cytoplasmic male sterileplant is a tobacco plant produced by growing the seed of tobaccocultivar CMS Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121054.

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is cytoplasmic male sterile (CMS), where thecytoplasmic male sterile plant is a tobacco plant produced by growingthe seed of tobacco cultivar CMS Banket A1 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121054.

In another aspect, the present disclosure further includes a harvestedleaf of an F₁ progeny plant having a reduced amount of nornicotineand/or N′-nitrosonornicotine (NNN) produced by growing a seed producedby crossing two tobacco plants and harvesting the resultant tobaccoseed, where at least one tobacco plant is cytoplasmic male sterile(CMS), where the cytoplasmic male sterile plant is a tobacco plantproduced by growing the seed of tobacco cultivar CMS Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121054.

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant having a reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN) in a smoke stream produced from the leaf,where the plant is produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is cytoplasmic male sterile (CMS), where thecytoplasmic male sterile plant is a tobacco plant produced by growingthe seed of tobacco cultivar CMS Banket A1 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121054.

In an aspect, the present disclosure includes a tobacco product preparedfrom an F₁ progeny tobacco plant, or part thereof, where the plant orpart thereof is produced by growing an F₁ progeny seed produced bycrossing two tobacco plants and harvesting the resultant tobacco seed,where at least one tobacco plant is cytoplasmic male sterile (CMS),where the cytoplasmic male sterile plant is a tobacco plant produced bygrowing the seed of tobacco cultivar CMS Banket A1 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121054, and where the tobacco product is selectedfrom the group consisting of pipe tobacco, cigar tobacco, cigarettetobacco, chewing tobacco, leaf tobacco, shredded tobacco, cut tobaccoand any combination thereof.

In an aspect, the present disclosure further includes a tobacco productprepared from an F₁ progeny tobacco plant, or part thereof, where theplant or part thereof is produced by growing an F₁ progeny seed producedby crossing two tobacco plants and harvesting the resultant tobaccoseed, where at least one tobacco plant is cytoplasmic male sterile(CMS), where the cytoplasmic male sterile plant is a tobacco plantproduced by growing the seed of tobacco cultivar CMS Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121054, and where the tobaccoproduct is selected from the group consisting of a cigarillo, anon-ventilated recess filter cigarette, a vented recess filtercigarette, a cigar, snuff, and chewing tobacco.

In an aspect, the present disclosure further includes a tobacco productprepared from an F₁ progeny tobacco plant, or part thereof, where theplant or part thereof is produced by growing an F₁ progeny seed producedby crossing two tobacco plants and harvesting the resultant tobaccoseed, where at least one tobacco plant is cytoplasmic male sterile(CMS), where the cytoplasmic male sterile plant is a tobacco plantproduced by growing the seed of tobacco cultivar CMS Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121054, and where the tobaccoproduct is selected from the group consisting of a cigarillo, anon-ventilated recess filter cigarette, a vented recess filtercigarette, a cigar, snuff, and chewing tobacco, and further where theproduct has a reduced amount of nornicotine and/or N′-nitrosonornicotine(NNN).

In an aspect, the present disclosure includes a method for producing atobacco seed comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is cytoplasmicmale sterile, where the cytoplasmic male sterile plant is a plant oftobacco cultivar CMS Banket A1 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121054.

In an aspect, the present disclosure includes a method of vegetativelypropagating a plant of a tobacco cultivar comprising the steps of (a)collecting tissue capable of being propagated from a plant of a tobaccocultivar Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121043or CMS Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121054;(b) cultivating the tissue to obtain a proliferated shoot; and (c)rooting the proliferated shoots to obtain a rooted plantlet.

In an aspect, the present disclosure includes a method of vegetativelypropagating a plant of a tobacco cultivar comprising the steps of (a)collecting tissue capable of being propagated from a plant of a tobaccocultivar Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121043or CMS Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121054;(b) cultivating the tissue to obtain a proliferated shoot; (c) rootingthe proliferated shoots to obtain a rooted plantlet; and (d) growing aplant from the rooted plantlet.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar Banket A1 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121043 or CMS Banket A1 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121054, with a second tobacco plant that comprises a desired traitto produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of said firsttobacco cultivar to produce a backcross BC₁F₁ progeny seed; (d) growingthe BC₁F₁ progeny seed and selecting a backcross BC₁F₁ progeny plantcomprising the desired trait and essentially all of the physiologicaland morphological characteristics of said first tobacco cultivar, BanketA1 SRC or CMS Banket A1 SRC; and (e) repeating steps (c) and (d) threeor more times (e.g., 3, 4, 5, 6, 7, 8, 9, 10, and the like) insuccession to produce selected fourth or higher backcross progeny thatcomprise the desired trait. In additional aspects, steps (c) and (d) canbe repeated one or more times (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, andthe like) in succession to produce second or higher backcross progenycomprising the desired trait.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar Banket A1 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121043, with a second tobacco plant that comprises a desiredtrait to produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of the first tobaccocultivar Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121043to produce a backcross BC₁F₁ progeny seed; (d) growing the BC₁F₁ progenyseed and selecting a backcross BC₁F₁ progeny plant comprising thedesired trait and essentially all of the physiological and morphologicalcharacteristics of the first tobacco cultivar, Banket A1 SRC; and (e)repeating steps (c) and (d) three or more times in succession to produceselected fourth or higher backcross progeny that comprise the desiredtrait, where the trait is cytoplasmic male sterility (CMS). Inadditional aspects, steps (c) and (d) can be repeated one or more timesin succession to produce second or higher backcross progeny comprisingthe desired trait, where the trait is CMS.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar Banket A1 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121043, with a second tobacco plant that comprises a desiredtrait to produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of the first tobaccocultivar Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121043to produce a backcross BC₁F₁ progeny seed; (d) growing the BC₁F₁ progenyseed and selecting a backcross BC₁F₁ progeny plant comprising thedesired trait and the physiological and essentially all of morphologicalcharacteristics of the first tobacco cultivar Banket A1 SRC; and (e)repeating steps (c) and (d) three or more times in succession to produceselected fourth or higher backcross progeny that comprise the desiredtrait, where the trait is cytoplasmic male sterility (CMS) and the CMStrait is obtained from the cytoplasm of Nicotiana suaveolens orNicotiana glauca. In additional aspects, steps (c) and (d) can berepeated one or more times in succession to produce second or higherbackcross progeny comprising the desired trait, where the trait is CMSand the CMS trait is obtained from the cytoplasm of Nicotiana suaveolensor Nicotiana glauca.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar Banket A1 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121043 with a second tobacco plant that comprises a desiredtrait to produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of the first tobaccocultivar, Banket A1 SRC to produce a backcross BC₁F₁ progeny seed; (d)growing the BC₁F₁ progeny seed and selecting a backcross BC₁F₁ progenyplant comprising the desired trait and essentially all of thephysiological and morphological characteristics of tobacco cultivarBanket A1 SRC; and (e) repeating steps (c) and (d) three or more timesin succession to produce selected fourth or higher backcross progenythat comprise the desired trait, where the trait is cytoplasmic malesterility (CMS) and the CMS trait is obtained from the cytoplasm ofNicotiana suaveolens, and where the second tobacco plant is CMS BanketA1 SRC. In additional aspects, steps (c) and (d) can be repeated one ormore times in succession to produce second or higher backcross progenycomprising the desired trait, where the trait is CMS and the CMS traitis obtained from the cytoplasm of Nicotiana suaveolens.

In an aspect, the present disclosure includes a tobacco plant producedby a method comprising introducing a desired trait into a tobaccocultivar comprising: (a) crossing a plant of a first tobacco cultivarBanket A1 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121043 or CMSBanket A1 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121054, with asecond tobacco plant that comprises a desired trait to produce an F₁progeny seed; (b) growing the F₁ progeny seed and selecting an F₁progeny plant that comprises the desired trait; (c) crossing theselected F₁ progeny plant with a plant of the first tobacco cultivarBanket A1 SRC or CMS Banket A1 SRC to produce a backcross BC₁F₁ progenyseed; (d) growing the BC₁F₁ progeny seed and selecting a backcross BC₁F₁progeny plant comprising the desired trait and essentially all of thephysiological and morphological characteristics of the first tobaccocultivar Banket A1 SRC or CMS Banket A1 SRC; and (e) repeating steps (c)and (d) three or more times in succession to produce selected fourth orhigher backcross progeny that comprise the desired trait. In additionalaspects, steps (c) and (d) can be repeated one or more times insuccession to produce second or higher backcross progeny that comprisethe desired trait.

In another aspect, the present disclosure includes a method ofintroducing a desired trait into a tobacco cultivar comprising: (a)crossing a plant of a first tobacco cultivar Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121043 or CMS Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121054 with a plant of a secondtobacco cultivar that comprises a desired trait to produce a progenyplant where the desired trait is selected from the group consisting ofherbicide resistance, pest resistance, disease resistance, high yield,high grade index, curability, curing quality, mechanical harvestability,holding ability, leaf quality, height, plant maturation, early maturing,early to medium maturing, medium maturing, medium to late maturing, latematuring, small stalk, medium stalk, large stalk, leaf number per plant,5-10 leaves per plant, 11-15 leaves per plant, 16-21 leaves per plant,and any combination thereof, to produce an F₁ progeny seed; (b) growingthe F₁ progeny seed into an F₁ progeny plant and selecting the F₁progeny plant having the desired trait; (c) crossing the selected F₁progeny plant with a plant of the first tobacco cultivar Banket A1 SRC,a representative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121043 or CMS Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121054 to produce a backcrossprogeny plant seed; (d) growing the backcross progeny plant seed into abackcross progeny plant and selecting the backcross progeny plantcomprising the desired trait and essentially all of the physiologicaland morphological characteristics of the first tobacco cultivar, BanketA1 SRC or CMS Banket A1 SRC; and (e) repeating steps (c) and (d) one ormore times in succession to produce a selected second, third, fourth orhigher backcross progeny plant that comprises the desired trait andessentially all of the physiological and morphological characteristicsof the first tobacco cultivar, Banket A1 SRC or CMS Banket A1 SRC.

In another aspect, the present disclosure includes a method ofintroducing a desired trait into a tobacco cultivar comprising: (a)crossing a plant of a first tobacco cultivar Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121043 or CMS Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121054, with a plant of a secondtobacco cultivar that comprises a desired trait to produce a progenyplant where the desired trait is selected from the group consisting ofherbicide resistance, pest resistance, disease resistance, high yield,high grade index, curability, curing quality, mechanical harvestability,holding ability, leaf quality, height, plant maturation, early maturing,early to medium maturing, medium maturing, medium to late maturing, latematuring, small stalk, medium stalk, large stalk, leaf number per plant,5-10 leaves per plant, 11-15 leaves per plant, 16-21 leaves per plant,and any combination thereof, to produce an F₁ progeny seed; (b) growingthe F₁ progeny seed into an F₁ progeny plant and selecting the F₁progeny plant having the desired trait; (c) crossing the selected F₁progeny plant with a plant of the first tobacco cultivar to produce abackcross progeny plant seed; (d) growing the backcross progeny plantseed into a backcross progeny plant and selecting the backcross progenyplant comprising the desired trait and essentially all of thephysiological and morphological characteristics of the first tobaccocultivar, Banket A1 SRC or CMS Banket A1 SRC; and (e) repeating steps(c) and (d) one or more times in succession to produce a selectedsecond, third, fourth or higher backcross progeny plant that comprisesthe desired trait and essentially all of the physiological andmorphological characteristics of the first tobacco cultivar, Banket A1SRC or CMS Banket A1 SRC.

In another aspect, the present disclosure includes a method ofintroducing a desired trait into a tobacco cultivar comprising: (a)crossing a plant of a first tobacco cultivar Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121043 or CMS Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121054, with a plant of a secondtobacco cultivar that comprises a desired trait to produce a progenyplant where the desired trait is selected from the group consisting ofherbicide resistance, pest resistance, disease resistance, high yield,high grade index, curability, curing quality, mechanical harvestability,holding ability, leaf quality, height, plant maturation, early maturing,early to medium maturing, medium maturing, medium to late maturing, latematuring, small stalk, medium stalk, large stalk, leaf number per plant,5-10 leaves per plant, 11-15 leaves per plant, 16-21 leaves per plant,and any combination thereof, to produce an F₁ progeny seed; (b) growingthe F₁ progeny seed into an F₁ progeny plant and selecting the F₁progeny plant having the desired trait; (c) crossing the selected F₁progeny plant with a plant of the first tobacco cultivar to produce abackcross progeny plant seed; (d) growing the backcross progeny plantseed into a backcross progeny plant and selecting the backcross progenyplant comprising the desired trait and essentially all of thephysiological and morphological characteristics of the first tobaccocultivar, Banket A1 SRC or CMS Banket A1 SRC; and (e) repeating steps(c) and (d) one or more times in succession to produce a selectedsecond, third, fourth or higher backcross progeny plant that comprisesthe desired trait and essentially all of the physiological andmorphological characteristics of the first tobacco cultivar, Banket A1SRC or CMS Banket A1 SRC, where the plant has a desired trait of diseaseresistance.

In another aspect, the present disclosure includes a method forproducing a tobacco plant having decreased nicotine conversioncomprising: identifying a first tobacco plant comprising a nucleotidesequence selected from the group consisting of the nucleotide sequenceof SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 11, and any combinationthereof; crossing the first tobacco plant with a second tobacco plantand collecting an F₁ seed; crossing a plant grown from the F₁ seed to athird tobacco plant and collecting a second tobacco seed; andidentifying a second tobacco seed or a plant grown from the second seedthat is homozygous for the nucleotide sequence selected from the groupconsisting of the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 11, and any combination thereof.

In an aspect, the present disclosure includes a method for producing atobacco plant having decreased nicotine conversion comprising:identifying a first tobacco plant comprising a nucleotide sequenceselected from the group consisting of the nucleotide sequence of SEQ IDNO: 1, SEQ ID NO: 2, SEQ ID NO: 11, and any combination thereof;crossing the first tobacco plant with a second tobacco plant andcollecting an F₁ seed; crossing a plant grown from the F₁ seed to athird tobacco plant and collecting a second tobacco seed; andidentifying a second tobacco seed or a plant grown from the second seedthat is homozygous for the nucleotide sequence selected from the groupconsisting of the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 11, and any combination thereof, where the second tobacco plantcomprises a nucleotide sequence selected from the group consisting ofthe nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 11,and any combination thereof.

In an aspect, the present disclosure includes a method for producing atobacco plant having decreased nicotine conversion comprising:identifying a first tobacco plant comprising a nucleotide sequenceselected from the group consisting of the nucleotide sequence of SEQ IDNO: 1, SEQ ID NO: 2, SEQ ID NO: 11, and any combination thereof;crossing the first tobacco plant with a second tobacco plant andcollecting an F₁ seed; crossing a plant grown from the F₁ seed to athird tobacco plant and collecting a second tobacco seed; andidentifying a second tobacco seed or a plant grown from the second seedthat is homozygous for the nucleotide sequence selected from the groupconsisting of the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 11, and any combination thereof, where the second tobacco plantdoes not have the nucleotide sequence selected from the group consistingof the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 11,and any combination thereof, and the third tobacco plant is a tobaccoplant comprising a nucleotide sequence selected from the groupconsisting of the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 11, and any combination thereof.

In an aspect, the present disclosure includes a method for producing atobacco plant having decreased nicotine conversion comprising:identifying a first tobacco plant comprising a nucleotide sequenceselected from the group consisting of the nucleotide sequence of SEQ IDNO: 1, SEQ ID NO: 2, SEQ ID NO: 11, and any combination thereof;crossing the first tobacco plant with a second tobacco plant andcollecting an F₁ seed; crossing a plant grown from the F₁ seed to athird tobacco plant and collecting a second tobacco seed; andidentifying a second tobacco seed or a plant grown from the second seedthat is homozygous for the nucleotide sequence selected from the groupconsisting of the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 11, and any combination thereof, where the first tobacco plantcomprises the sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 2.

In an aspect, the present disclosure includes a method for producing atobacco plant having decreased nicotine conversion comprising:identifying a first tobacco plant comprising a nucleotide sequenceselected from the group consisting of the nucleotide sequence of SEQ IDNO: 1, SEQ ID NO: 2, SEQ ID NO: 11, and any combination thereof;crossing the first tobacco plant with a second tobacco plant andcollecting an F₁ seed; crossing a plant grown from the F₁ seed to athird tobacco plant and collecting a second tobacco seed; andidentifying a second tobacco seed or a plant grown from the second seedthat is homozygous for the nucleotide sequence selected from the groupconsisting of the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 11, and any combination thereof, where the third tobacco plant isa tobacco plant comprising a nucleotide sequence selected from the groupconsisting of the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 11, and any combination thereof.

In an aspect, the present disclosure includes a method for producing atobacco plant having decreased nicotine conversion comprising:identifying a first tobacco plant comprising a nucleotide sequenceselected from the group consisting of the nucleotide sequence of SEQ IDNO: 1, SEQ ID NO: 2, SEQ ID NO: 11, and any combination thereof;crossing the first tobacco plant with a second tobacco plant andcollecting an F₁ seed; crossing a plant grown from the F₁ seed to athird tobacco plant and collecting a second tobacco seed; andidentifying a second tobacco seed or a plant grown from the second seedthat is homozygous for the nucleotide sequence selected from the groupconsisting of the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 11, and any combination thereof, where the first tobacco plant isa plant of tobacco cultivar Banket A1 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121043, or tobacco cultivar CMS Banket A1 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121054.

In an aspect, the present disclosure includes a method for producing atobacco plant having decreased nicotine conversion comprising:identifying a first tobacco plant comprising a nucleotide sequenceselected from the group consisting of the nucleotide sequence of SEQ IDNO: 1, SEQ ID NO: 2, SEQ ID NO: 11, and any combination thereof;crossing the first tobacco plant with a second tobacco plant andcollecting an F₁ seed; crossing a plant grown from the F₁ seed to athird tobacco plant and collecting a second tobacco seed; andidentifying a second tobacco seed or a plant grown from the second seedthat is homozygous for the nucleotide sequence selected from the groupconsisting of the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 11, and any combination thereof, where the third tobacco plant isa plant of tobacco cultivar Banket A1 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121043, or tobacco cultivar CMS Banket A1 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121054.

In another aspect, the present disclosure includes a method of producinga plant of a tobacco cultivar Banket A1 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121043 or tobacco cultivar CMS Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121054 comprising at least one(e.g., 1, 2, 3, 4, 5, 6, 7, 8, and the like) additional desired traitcomprising the steps of: (a) collecting tissue capable of beingpropagated from a plant of a tobacco cultivar Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121043 or CMS Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121054; and (b) introducing a atleast one transgene (nucleic acid construct) conferring at least onedesired trait into the tissue.

In another aspect, the present disclosure includes a method of producingan herbicide resistant tobacco plant comprising transforming a tobaccoplant, or part thereof, produced by growing a seed of tobacco cultivarBanket A1 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121043 or tobaccocultivar CMS Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121054with at least one transgene (nucleic acid construct), where the at leastone transgene (nucleic acid construct) confers resistance to anherbicide selected from the group consisting of imidazolinone,cyclohexanedione, sulfonylurea, glyphosate, glufosinate, phenoxyproprionic acid, L-phosphinothricin, triazine, benzonitrile, and anycombination thereof.

In another aspect, the present disclosure includes an herbicideresistant tobacco plant produced by a method comprising transforming atobacco plant, or part thereof, produced by growing a seed of a tobaccocultivar Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121043or CMS Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121054with at least one transgene (nucleic acid construct), where the at leastone transgene (nucleic acid construct) confers resistance to anherbicide selected from the group consisting of imidazolinone,cyclohexanedione, sulfonylurea, glyphosate, glufosinate, phenoxyproprionic acid, L-phosphinothricin, triazine, benzonitrile, and anycombination thereof.

In another aspect, the present disclosure includes a method of producinga pest and/or insect resistant tobacco plant where the method comprisestransforming a tobacco plant produced by growing a seed of tobaccocultivar Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121043or tobacco cultivar CMS Banket A1 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121054, with at least one transgene (nucleic acid construct)that confers pest and/or insect resistance.

In a further aspect, the present disclosure includes a pest and/orinsect resistant tobacco plant produced by a method comprisingtransforming a tobacco plant produced by growing a seed of tobaccocultivar Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121043or tobacco cultivar CMS Banket A1 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121054, with at least one transgene (nucleic acid construct)that confers pest and/or insect resistance.

In a further aspect, the present disclosure includes a pest and/orinsect resistant tobacco plant produced by a method comprisingtransforming a tobacco plant produced by growing a seed of tobaccocultivar Banket A1 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121043or tobacco cultivar CMS Banket A1 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121054, with at least one transgene (nucleic acid construct)that confers pest and/or insect resistance, where the transgene (nucleicacid construct) encodes a Bacillus thuringiensis (BT) endotoxin.

In another aspect, the present disclosure includes a method of producinga disease resistant tobacco plant, the method comprising transforming atobacco plant produced by growing a seed of tobacco cultivar Banket A1SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121043 or tobacco cultivarCMS Banket A1 SRC, a representative sample seed of the cultivar havingbeen deposited with the ATCC under ATCC Accession No. PTA-121054 with atleast one transgene (nucleic acid construct) that confers diseaseresistance.

In a further aspect, the present disclosure includes a disease resistanttobacco plant produced by transforming a tobacco plant produced bygrowing a seed of tobacco cultivar Banket A1 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121043 or tobacco cultivar CMS Banket A1 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121054 with at least one transgene(nucleic acid construct) that confers disease resistance.

In an aspect, the present disclosure includes a seed of tobacco cultivarNC 2000 SRC, a representative sample seed of the cultivar having beendeposited with the American Type Culture Collection (ATCC) under ATCCAccession No. PTA-121053.

In another aspect, the present disclosure includes a tobacco plant, orpart thereof, produced by growing the seed of tobacco cultivar NC 2000SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121053.

In a further aspect, the present disclosure includes a harvested leaf,or part thereof, of a tobacco plant, or part thereof, produced bygrowing the seed of tobacco cultivar NC 2000 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121053.

In an aspect, the present disclosure includes a harvested leaf, or partthereof, of a tobacco plant, produced by growing the seed of tobaccocultivar NC 2000 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121053,where the leaf has a reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN).

In an aspect, the present disclosure includes a harvested leaf, or partthereof, produced by growing the seed of tobacco cultivar NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121053, where the leaf has areduced amount of nornicotine and/or N-nitrosonomicotine (NNN), wherethe reduced amount of nornicotine and/or N′-nitrosonornicotine (NNN) isreduced in a smoke stream produced from the leaf.

In a further aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar NC 2000 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121053, where the product is selected from the group consistingof pipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof.

In an aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar NC 2000 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121053, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco.

In another aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar NC 2000 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121053, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco, and wherethe product has a reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN).

In a further aspect, the present disclosure includes a second tobaccoproduct prepared or produced from a first tobacco product prepared froma tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121053, where first product is selected from the group consisting ofpipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof, andthe second tobacco product is selected from the group consisting of acigarillo, a non-ventilated recess filter cigarette, a vented recessfilter cigarette, a cigar, snuff, and chewing tobacco. In some aspects,the first and second product comprises a reduced amount of nornicotineand/or N′-nitrosonornicotine (NNN).

In an aspect, the present disclosure includes a part of a tobacco plant,produced by growing the seed of tobacco cultivar NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121053, where the part is selectedfrom the group consisting of leaf, pollen, ovule, embryo, cotyledon,hypocotyl, meristematic cell, protoplast, root, root tip, pistil,anther, flower, shoot, stem, pod and petiole.

In another aspect, the present disclosure includes a tissue cultureproduced from a protoplast or cell from a tobacco plant, or partthereof, produced by growing the seed of tobacco cultivar NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121053, where the cell orprotoplast of the tissue culture is produced from a plant part selectedfrom the group consisting of a leaf, pollen, embryo, cotyledon,hypocotyl, meristematic cell, root, root tip, pistil, anther, flower,shoot, stem, pod, and petiole.

In an aspect, the present disclosure includes a tobacco plantregenerated from a tissue culture produced from a protoplast or cellfrom a tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121053, where the cell or protoplast of the tissue culture isproduced from a plant part selected from the group consisting of a leaf,pollen, embryo, cotyledon, hypocotyl, meristematic cell, root, root tip,pistil, anther, flower, shoot, stem, pod, and petiole where theregenerated plant has all, or essentially all of the morphological andphysiological characteristics of cultivar NC 2000 SRC.

In an aspect, the present disclosure includes an F₁ progeny plant oftobacco cultivar NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121053.

In another aspect, the present disclosure includes an F₁ progeny plantof tobacco cultivar NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121053, where the F₁ plant is cytoplasmic male sterile (CMS).

In another aspect, the present disclosure includes an F₁ progeny seedproduced by crossing two tobacco plants and harvesting the resultanttobacco seed, where at least one tobacco plant is a tobacco plantproduced by growing the seed of tobacco cultivar NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121053, where at least one tobaccoplant is cytoplasmic male sterile (CMS).

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar NC2000 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121053.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar NC2000 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121053, where theplant of tobacco cultivar NC 2000 SRC is the male parent.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar NC2000 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121053, where theplant of tobacco cultivar NC 2000 SRC is the female parent.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar NC2000 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121053, where atleast one tobacco plant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure also includes a container of F₁progeny seeds produced by crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is a tobaccoplant produced by growing the seed of tobacco cultivar NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121053, where at least one tobaccoplant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure includes an F₁ progeny plantproduced by growing a seed produced by crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar NC2000 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121053, and whereat least one tobacco plant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is a tobacco plant produced by growing the seed oftobacco cultivar NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121053, where at least one tobacco plant is cytoplasmic male sterile(CMS).

In another aspect, the present disclosure further includes a harvestedleaf of an F₁ progeny plant having a reduced amount of nornicotineand/or N′-nitrosonornicotine (NNN) produced by growing a seed producedby a method of comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is a tobaccoplant produced by growing the seed of tobacco cultivar NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121053, where at least one tobaccoplant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant having a reduced amount of nornicotine and/orN′-nitrosonomicotine (NNN) in a smoke stream produced from the leaf,where the plant is produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is a tobacco plant produced by growing the seed oftobacco cultivar NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121053, where at least one tobacco plant is cytoplasmic male sterile(CMS).

In an aspect, the present disclosure includes a tobacco product preparedfrom an F₁ progeny tobacco plant, or part thereof, where the plant orpart thereof is produced by growing an F₁ progeny seed produced by amethod comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is produced bygrowing a seed of tobacco cultivar NC 2000 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121053, where at least one tobacco plant iscytoplasmic male sterile, and where the tobacco product is selected fromthe group consisting of pipe tobacco, cigar tobacco, cigarette tobacco,chewing tobacco, leaf tobacco, shredded tobacco, cut tobacco, and anycombination thereof.

In an aspect, the present disclosure further includes a tobacco productprepared from an F₁ progeny tobacco plant, or part thereof, where theplant or part thereof is produced by growing an F₁ progeny seed producedby a method comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is produced bygrowing a seed of tobacco cultivar NC 2000 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121053, where at least one tobacco plant iscytoplasmic male sterile, and where the tobacco product is selected fromthe group consisting of a cigarillo, a non-ventilated recess filtercigarette, a vented recess filter cigarette, a cigar, snuff, and chewingtobacco.

In another aspect, the present disclosure includes a seed of tobaccocultivar CMS NC 2000 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121045.

In an aspect, the present disclosure includes a tobacco plant, or partthereof, produced by growing the seed of tobacco cultivar CMS NC 2000SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121045.

In another aspect, the present disclosure includes a harvested leaf, orpart thereof, of a tobacco plant, or part thereof, produced by growingthe seed of tobacco cultivar CMS NC 2000 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121045.

In an aspect, the present disclosure includes a harvested leaf, or partthereof, of a tobacco plant, produced by growing the seed of tobaccocultivar CMS NC 2000 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121045,where the leaf has a reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN).

In another aspect, the present disclosure includes a harvested leaf, orpart thereof, produced by growing the seed of tobacco cultivar CMS NC2000 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121045, where theleaf has a reduced amount of nornicotine and/or N′-nitrosonornicotine(NNN), where the reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN) is reduced in a smoke stream produced fromthe leaf.

In a further aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar CMS NC 2000 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121045, where the product is selected from the group consistingof pipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof.

In an aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar CMS NC 2000 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121045, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco.

In another aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar CMS NC 2000 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121045, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco where theproduct has a reduced amount of nornicotine and/or N′-nitrosonornicotine(NNN).

In a further aspect, the present disclosure includes a second tobaccoproduct prepared or produced from a first tobacco product prepared froma tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar CMS NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121045, where first product is selected from the group consisting ofpipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof, andthe second tobacco product is selected from the group consisting of acigarillo, a non-ventilated recess filter cigarette, a vented recessfilter cigarette, a cigar, snuff, and chewing tobacco. In some aspects,the first and/or the second product comprises a reduced amount ofnornicotine and/or N′-nitrosonornicotine (NNN).

In an aspect, the present disclosure includes a part of a tobacco plant,produced by growing the seed of tobacco cultivar CMS NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121045, where the part is selectedfrom the group consisting of leaf, pollen, ovule, embryo, cotyledon,hypocotyl, meristematic cell, protoplast, root, root tip, pistil,anther, flower, shoot, stem, pod, and petiole.

In another aspect, the present disclosure includes a tissue cultureproduced from a protoplast or cell from a tobacco plant, or partthereof, produced by growing the seed of tobacco cultivar CMS NC 2000SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121045, where the cell orprotoplast of the tissue culture is produced from a plant part selectedfrom the group consisting of a leaf, pollen, embryo, cotyledon,hypocotyl, meristematic cell, root, root tip, pistil, anther, flower,shoot, stem, pod, and petiole.

In an aspect, the present disclosure includes a tobacco plantregenerated from a tissue culture produced from a protoplast or cellfrom a tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar CMS NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121045, where the cell or protoplast of the tissue culture can beproduced from a plant part selected from the group consisting of a leaf,pollen, embryo, cotyledon, hypocotyl, meristematic cell, root, root tip,pistil, anther, flower, shoot, stem, pod, and petiole where theregenerated plant has all, or essentially all of the morphological andphysiological characteristics of cultivar CMS NC 2000 SRC.

In an aspect, the present disclosure includes an F₁ progeny plant oftobacco cultivar CMS NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121045.

In another aspect, the present disclosure includes an F₁ progeny plantof tobacco cultivar CMS NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121045, where the F₁ plant is cytoplasmic male sterile (CMS).

In another aspect, the present disclosure includes an F₁ progeny seedproduced by crossing two tobacco plants and harvesting the resultanttobacco seed, where at least one tobacco plant is cytoplasmic malesterile (CMS), where the cytoplasmic male sterile plant is a plant oftobacco cultivar CMS NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121045.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar CMSNC 2000 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121045.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar CMSNC 2000 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121045, where theplant of tobacco cultivar CMS NC 2000 SRC is the female parent.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis cytoplasmic male sterile (CMS), where the cytoplasmic male sterileplant is a plant of tobacco cultivar CMS NC 2000 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121045.

In an aspect, the present disclosure also includes a container of F₁progeny seeds produced by crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is cytoplasmicmale sterile (CMS), where the cytoplasmic male sterile plant is atobacco plant produced by growing the seed of tobacco cultivar CMS NC2000 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121045.

In an aspect, the present disclosure includes an F₁ progeny plantproduced by growing a seed produced by crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis cytoplasmic male sterile (CMS), where the cytoplasmic male sterileplant is a tobacco plant produced by growing the seed of tobaccocultivar CMS NC 2000 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121045.

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is cytoplasmic male sterile (CMS), where thecytoplasmic male sterile plant is a tobacco plant produced by growingthe seed of tobacco cultivar CMS NC 2000 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121045.

In another aspect, the present disclosure further includes a harvestedleaf of an F₁ progeny plant having a reduced amount of nornicotineand/or N′-nitrosonomicotine (NNN) produced by growing a seed produced bycrossing two tobacco plants and harvesting the resultant tobacco seed,where at least one tobacco plant is cytoplasmic male sterile (CMS),where the cytoplasmic male sterile plant is a tobacco plant produced bygrowing the seed of tobacco cultivar CMS NC 2000 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121045.

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant having a reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN) in a smoke stream produced from the leaf,where the plant is produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is cytoplasmic male sterile (CMS), where thecytoplasmic male sterile plant is a tobacco plant produced by growingthe seed of tobacco cultivar CMS NC 2000 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121045.

In an aspect, the present disclosure includes a tobacco product preparedfrom an F₁ progeny tobacco plant, or part thereof, where the plant orpart thereof is produced by growing an F₁ progeny seed produced bycrossing two tobacco plants and harvesting the resultant tobacco seed,where at least one tobacco plant is cytoplasmic male sterile (CMS),where the cytoplasmic male sterile plant is a tobacco plant produced bygrowing the seed of tobacco cultivar CMS NC 2000 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121045, and where the tobacco product is selectedfrom the group consisting of pipe tobacco, cigar tobacco, cigarettetobacco, chewing tobacco, leaf tobacco, shredded tobacco, cut tobaccoand any combination thereof.

In an aspect, the present disclosure further includes a tobacco productprepared from an F₁ progeny tobacco plant, or part thereof, where theplant or part thereof is produced by growing an F₁ progeny seed producedby crossing two tobacco plants and harvesting the resultant tobaccoseed, where at least one tobacco plant is cytoplasmic male sterile(CMS), where the cytoplasmic male sterile plant is a tobacco plantproduced by growing the seed of tobacco cultivar CMS NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121045, and where the tobaccoproduct is selected from the group consisting of a cigarillo, anon-ventilated recess filter cigarette, a vented recess filtercigarette, a cigar, snuff, and chewing tobacco.

In an aspect, the present disclosure further includes a tobacco productprepared from an F₁ progeny tobacco plant, or part thereof, where theplant or part thereof is produced by growing an F₁ progeny seed producedby crossing two tobacco plants and harvesting the resultant tobaccoseed, where at least one tobacco plant is cytoplasmic male sterile(CMS), where the cytoplasmic male sterile plant is a tobacco plantproduced by growing the seed of tobacco cultivar CMS NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121045, and where the tobaccoproduct is selected from the group consisting of a cigarillo, anon-ventilated recess filter cigarette, a vented recess filtercigarette, a cigar, snuff, and chewing tobacco, and further where theproduct has a reduced amount of nornicotine and/or N′-nitrosonornicotine(NNN).

In an aspect, the present disclosure includes a method for producing atobacco seed comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is cytoplasmicmale sterile, where the cytoplasmic male sterile plant is a plant oftobacco cultivar CMS NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121045.

In an aspect, the present disclosure includes a method of vegetativelypropagating a plant of a tobacco cultivar comprising the steps of (a)collecting tissue capable of being propagated from a plant of a tobaccocultivar NC 2000 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121053or CMS NC 2000 SRC, a representative sample seed of the cultivar havingbeen deposited with the ATCC under ATCC Accession No. PTA-121045; (b)cultivating the tissue to obtain a proliferated shoot; and (c) rootingthe proliferated shoots to obtain a rooted plantlet.

In an aspect, the present disclosure includes a method of vegetativelypropagating a plant of a tobacco cultivar comprising the steps of (a)collecting tissue capable of being propagated from a plant of a tobaccocultivar NC 2000 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121053or CMS NC 2000 SRC, a representative sample seed of the cultivar havingbeen deposited with the ATCC under ATCC Accession No. PTA-121045; (b)cultivating the tissue to obtain a proliferated shoot; (c) rooting theproliferated shoots to obtain a rooted plantlet; and (d) growing a plantfrom the rooted plantlet.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar NC 2000 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121053 or CMS NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121045, with a second tobacco plant that comprises a desired traitto produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of said firsttobacco cultivar to produce a backcross BC₁F₁ progeny seed; (d) growingthe BC₁F₁ progeny seed and selecting a backcross BC₁F₁ progeny plantcomprising the desired trait and essentially all of the physiologicaland morphological characteristics of said first tobacco cultivar, NC2000 SRC or CMS NC 2000 SRC; and (e) repeating steps (c) and (d) threeor more times (e.g., 3, 4, 5, 6, 7, 8, 9, 10, and the like) insuccession to produce selected fourth or higher backcross progeny thatcomprise the desired trait. In additional aspects, steps (c) and (d) canbe repeated one or more times (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, andthe like) in succession to produce second or higher backcross progenycomprising the desired trait.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar NC 2000 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121053, with a second tobacco plant that comprises a desiredtrait to produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of the first tobaccocultivar NC 2000 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121053to produce a backcross BC₁F₁ progeny seed; (d) growing the BC₁F₁ progenyseed and selecting a backcross BC₁F₁ progeny plant comprising thedesired trait and essentially all of the physiological and morphologicalcharacteristics of the first tobacco cultivar, NC 2000 SRC; and (e)repeating steps (c) and (d) three or more times in succession to produceselected fourth or higher backcross progeny that comprise the desiredtrait, where the trait is cytoplasmic male sterility (CMS). Inadditional aspects, steps (c) and (d) can be repeated one or more timesin succession to produce second or higher backcross progeny comprisingthe desired trait, where the trait is CMS.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar NC 2000 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121053, with a second tobacco plant that comprises a desiredtrait to produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of the first tobaccocultivar NC 2000 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121053to produce a backcross BC₁F₁ progeny seed; (d) growing the BC₁F₁ progenyseed and selecting a backcross BC₁F₁ progeny plant comprising thedesired trait and the physiological and essentially all of morphologicalcharacteristics of the first tobacco cultivar NC 2000 SRC; and (e)repeating steps (c) and (d) three or more times in succession to produceselected fourth or higher backcross progeny that comprise the desiredtrait, where the trait is cytoplasmic male sterility (CMS) and the CMStrait is obtained from the cytoplasm of Nicotiana suaveolens orNicotiana glauca. In additional aspects, steps (c) and (d) can berepeated one or more times in succession to produce second or higherbackcross progeny comprising the desired trait, where the trait is CMSand the CMS trait is obtained from the cytoplasm of Nicotiana suaveolensor Nicotiana glauca.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar NC 2000 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121053 with a second tobacco plant that comprises a desiredtrait to produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of the first tobaccocultivar, NC 2000 SRC to produce a backcross BC₁F₁ progeny seed; (d)growing the BC₁F₁ progeny seed and selecting a backcross BC₁F₁ progenyplant comprising the desired trait and essentially all of thephysiological and morphological characteristics of tobacco cultivar NC2000 SRC; and (e) repeating steps (c) and (d) three or more times insuccession to produce selected fourth or higher backcross progeny thatcomprise the desired trait, where the trait is cytoplasmic malesterility (CMS) and the CMS trait is obtained from the cytoplasm ofNicotiana suaveolens, and where the second tobacco plant is CMS NC 2000SRC. In additional aspects, steps (c) and (d) can be repeated one ormore times in succession to produce second or higher backcross progenycomprising the desired trait, where the trait is CMS and the CMS traitis obtained from the cytoplasm of Nicotiana suaveolens.

In an aspect, the present disclosure includes a tobacco plant producedby a method comprising introducing a desired trait into a tobaccocultivar comprising: (a) crossing a plant of a first tobacco cultivar NC2000 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121053 or CMS NC2000 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121045, with asecond tobacco plant that comprises a desired trait to produce an F₁progeny seed; (b) growing the F₁ progeny seed and selecting an F₁progeny plant that comprises the desired trait; (c) crossing theselected F₁ progeny plant with a plant of the first tobacco cultivar NC2000 SRC or CMS NC 2000 SRC to produce a backcross BC₁F₁ progeny seed;(d) growing the BC₁F₁ progeny seed and selecting a backcross BC₁F₁progeny plant comprising the desired trait and essentially all of thephysiological and morphological characteristics of the first tobaccocultivar NC 2000 SRC or CMS NC 2000 SRC; and (e) repeating steps (c) and(d) three or more times in succession to produce selected fourth orhigher backcross progeny that comprise the desired trait. In additionalaspects, steps (c) and (d) can be repeated one or more times insuccession to produce second or higher backcross progeny that comprisethe desired trait.

In another aspect, the present disclosure includes a method ofintroducing a desired trait into a tobacco cultivar comprising: (a)crossing a plant of a first tobacco cultivar NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121053 or CMS NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121045 with a plant of a secondtobacco cultivar that comprises a desired trait to produce a progenyplant where the desired trait is selected from the group consisting ofherbicide resistance, pest resistance, disease resistance, high yield,high grade index, curability, curing quality, mechanical harvestability,holding ability, leaf quality, height, plant maturation, early maturing,early to medium maturing, medium maturing, medium to late maturing, latematuring, small stalk, medium stalk, large stalk, leaf number per plant,5-10 leaves per plant, 11-15 leaves per plant, 16-21 leaves per plant,and any combination thereof, to produce an F₁ progeny seed; (b) growingthe F₁ progeny seed into an F₁ progeny plant and selecting the F₁progeny plant having the desired trait; (c) crossing the selected F₁progeny plant with a plant of the first tobacco cultivar NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121053 or CMS NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121045 to produce a backcrossprogeny plant seed; (d) growing the backcross progeny plant seed into abackcross progeny plant and selecting the backcross progeny plantcomprising the desired trait and essentially all of the physiologicaland morphological characteristics of the first tobacco cultivar, NC 2000SRC or CMS NC 2000 SRC; and (e) repeating steps (c) and (d) one or moretimes in succession to produce a selected second, third, fourth orhigher backcross progeny plant that comprises the desired trait andessentially all of the physiological and morphological characteristicsof the first tobacco cultivar, NC 2000 SRC or CMS NC 2000 SRC.

In another aspect, the present disclosure includes a method ofintroducing a desired trait into a tobacco cultivar comprising: (a)crossing a plant of a first tobacco cultivar NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121053 or CMS NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121045, with a plant of a secondtobacco cultivar that comprises a desired trait to produce a progenyplant where the desired trait is selected from the group consisting ofherbicide resistance, pest resistance, disease resistance, high yield,high grade index, curability, curing quality, mechanical harvestability,holding ability, leaf quality, height, plant maturation, early maturing,early to medium maturing, medium maturing, medium to late maturing, latematuring, small stalk, medium stalk, large stalk, leaf number per plant,5-10 leaves per plant, 11-15 leaves per plant, 16-21 leaves per plant,and any combination thereof, to produce an F₁ progeny seed; (b) growingthe F₁ progeny seed into an F₁ progeny plant and selecting the F₁progeny plant having the desired trait; (c) crossing the selected F₁progeny plant with a plant of the first tobacco cultivar to produce abackcross progeny plant seed; (d) growing the backcross progeny plantseed into a backcross progeny plant and selecting the backcross progenyplant comprising the desired trait and essentially all of thephysiological and morphological characteristics of the first tobaccocultivar, NC 2000 SRC or CMS NC 2000 SRC; and (e) repeating steps (c)and (d) one or more times in succession to produce a selected second,third, fourth or higher backcross progeny plant that comprises thedesired trait and essentially all of the physiological and morphologicalcharacteristics of the first tobacco cultivar, NC 2000 SRC or CMS NC2000 SRC.

In another aspect, the present disclosure includes a method ofintroducing a desired trait into a tobacco cultivar comprising: (a)crossing a plant of a first tobacco cultivar NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121053 or CMS NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121045, with a plant of a secondtobacco cultivar that comprises a desired trait to produce a progenyplant where the desired trait is selected from the group consisting ofherbicide resistance, pest resistance, disease resistance, high yield,high grade index, curability, curing quality, mechanical harvestability,holding ability, leaf quality, height, plant maturation, early maturing,early to medium maturing, medium maturing, medium to late maturing, latematuring, small stalk, medium stalk, large stalk, leaf number per plant,5-10 leaves per plant, 11-15 leaves per plant, 16-21 leaves per plant,and any combination thereof, to produce an F₁ progeny seed; (b) growingthe F₁ progeny seed into an F₁ progeny plant and selecting the F₁progeny plant having the desired trait; (c) crossing the selected F₁progeny plant with a plant of the first tobacco cultivar to produce abackcross progeny plant seed; (d) growing the backcross progeny plantseed into a backcross progeny plant and selecting the backcross progenyplant comprising the desired trait and essentially all of thephysiological and morphological characteristics of the first tobaccocultivar, NC 2000 SRC or CMS NC 2000 SRC; and (e) repeating steps (c)and (d) one or more times in succession to produce a selected second,third, fourth or higher backcross progeny plant that comprises thedesired trait and essentially all of the physiological and morphologicalcharacteristics of the first tobacco cultivar, NC 2000 SRC or CMS NC2000 SRC, where the plant has a desired trait of disease resistance.

In an aspect, the present disclosure includes a method for producing atobacco plant having decreased nicotine conversion comprising:identifying a first tobacco plant comprising a nucleotide sequenceselected from the group consisting of the nucleotide sequence of SEQ IDNO: 1, SEQ ID NO: 2, SEQ ID NO: 11, and any combination thereof;crossing the first tobacco plant with a second tobacco plant andcollecting an F₁ seed; crossing a plant grown from the F₁ seed to athird tobacco plant and collecting a second tobacco seed; andidentifying a second tobacco seed or a plant grown from the second seedthat is homozygous for the nucleotide sequence selected from the groupconsisting of the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 11, and any combination thereof, where the first tobacco plant isa plant of tobacco cultivar NC 2000 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121053, or tobacco cultivar CMS NC 2000 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121045.

In an aspect, the present disclosure includes a method for producing atobacco plant having decreased nicotine conversion comprising:identifying a first tobacco plant comprising a nucleotide sequenceselected from the group consisting of the nucleotide sequence of SEQ IDNO: 1, SEQ ID NO: 2, SEQ ID NO: 11, and any combination thereof;crossing the first tobacco plant with a second tobacco plant andcollecting an F₁ seed; crossing a plant grown from the F₁ seed to athird tobacco plant and collecting a second tobacco seed; andidentifying a second tobacco seed or a plant grown from the second seedthat is homozygous for the nucleotide sequence selected from the groupconsisting of the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 11, and any combination thereof, where the third tobacco plant isa plant of tobacco cultivar NC 2000 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121053, or tobacco cultivar CMS NC 2000 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121045.

In another aspect, the present disclosure includes a method of producinga plant of a tobacco cultivar NC 2000 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121053 or tobacco cultivar CMS NC 2000 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121045 comprising at least one (e.g., 1, 2, 3, 4,5, 6, 7, 8, and the like) additional desired trait comprising the stepsof: (a) collecting tissue capable of being propagated from a plant of atobacco cultivar NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121053 or CMS NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121045; and (b) introducing a at least one transgene (nucleic acidconstruct) conferring at least one desired trait into the tissue.

In another aspect, the present disclosure includes a method of producingan herbicide resistant tobacco plant comprising transforming a tobaccoplant, or part thereof, produced by growing a seed of tobacco cultivarNC 2000 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121053 or tobaccocultivar CMS NC 2000 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121045with at least one transgene (nucleic acid construct), where the at leastone transgene (nucleic acid construct) confers resistance to anherbicide selected from the group consisting of imidazolinone,cyclohexanedione, sulfonylurea, glyphosate, glufosinate, phenoxyproprionic acid, L-phosphinothricin, triazine, benzonitrile, and anycombination thereof.

In another aspect, the present disclosure includes an herbicideresistant tobacco plant produced by a method comprising transforming atobacco plant, or part thereof, produced by growing a seed of a tobaccocultivar NC 2000 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121053or CMS NC 2000 SRC, a representative sample seed of the cultivar havingbeen deposited with the ATCC under ATCC Accession No. PTA-121045 with atleast one transgene (nucleic acid construct), where the at least onetransgene (nucleic acid construct) confers resistance to an herbicideselected from the group consisting of imidazolinone, cyclohexanedione,sulfonylurea, glyphosate, glufosinate, phenoxy proprionic acid,L-phosphinothricin, triazine, benzonitrile, and any combination thereof.

In another aspect, the present disclosure includes a method of producinga pest and/or insect resistant tobacco plant where the method comprisestransforming a tobacco plant produced by growing a seed of tobaccocultivar NC 2000 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121053or tobacco cultivar CMS NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121045, with at least one transgene (nucleic acid construct) thatconfers pest and/or insect resistance.

In a further aspect, the present disclosure includes a pest and/orinsect resistant tobacco plant produced by a method comprisingtransforming a tobacco plant produced by growing a seed of tobaccocultivar NC 2000 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121053or tobacco cultivar CMS NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121045, with at least one transgene (nucleic acid construct) thatconfers pest and/or insect resistance.

In a further aspect, the present disclosure includes a pest and/orinsect resistant tobacco plant produced by a method comprisingtransforming a tobacco plant produced by growing a seed of tobaccocultivar NC 2000 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121053or tobacco cultivar CMS NC 2000 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121045, with at least one transgene (nucleic acid construct) thatconfers pest and/or insect resistance, where the transgene (nucleic acidconstruct) encodes a Bacillus thuringiensis (BT) endotoxin.

In another aspect, the present disclosure includes a method of producinga disease resistant tobacco plant, the method comprising transforming atobacco plant produced by growing a seed of tobacco cultivar NC 2000SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121053 or tobacco cultivarCMS NC 2000 SRC, a representative sample seed of the cultivar havingbeen deposited with the ATCC under ATCC Accession No. PTA-121045 with atleast one transgene (nucleic acid construct) that confers diseaseresistance.

In a further aspect, the present disclosure includes a disease resistanttobacco plant produced by transforming a tobacco plant produced bygrowing a seed of tobacco cultivar NC 2000 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121053 or tobacco cultivar CMS NC 2000 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121045 with at least one transgene(nucleic acid construct) that confers disease resistance.

In an aspect, the present disclosure includes a seed of tobacco cultivarNC 2002 SRC, a representative sample seed of the cultivar having beendeposited with the American Type Culture Collection (ATCC) under ATCCAccession No. PTA-121058.

In another aspect, the present disclosure includes a tobacco plant, orpart thereof, produced by growing the seed of tobacco cultivar NC 2002SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121058.

In a further aspect, the present disclosure includes a harvested leaf,or part thereof, of a tobacco plant, or part thereof, produced bygrowing the seed of tobacco cultivar NC 2002 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121058.

In an aspect, the present disclosure includes a harvested leaf, or partthereof, of a tobacco plant, produced by growing the seed of tobaccocultivar NC 2002 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121058,where the leaf has a reduced amount of nornicotine and/orN′-nitrosonomicotine (NNN).

In an aspect, the present disclosure includes a harvested leaf, or partthereof, produced by growing the seed of tobacco cultivar NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121058, where the leaf has areduced amount of nornicotine and/or N′-nitrosonornicotine (NNN), wherethe reduced amount of nornicotine and/or N′-nitrosonornicotine (NNN) isreduced in a smoke stream produced from the leaf.

In a further aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar NC 2002 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121058, where the product is selected from the group consistingof pipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof.

In an aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar NC 2002 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121058, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco.

In another aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar NC 2002 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121058, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco, and wherethe product has a reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN).

In a further aspect, the present disclosure includes a second tobaccoproduct prepared or produced from a first tobacco product prepared froma tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121058, where first product is selected from the group consisting ofpipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof, andthe second tobacco product is selected from the group consisting of acigarillo, a non-ventilated recess filter cigarette, a vented recessfilter cigarette, a cigar, snuff, and chewing tobacco. In some aspects,the first and second product comprises a reduced amount of nornicotineand/or N′-nitrosonornicotine (NNN).

In an aspect, the present disclosure includes a part of a tobacco plant,produced by growing the seed of tobacco cultivar NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121058, where the part is selectedfrom the group consisting of leaf, pollen, ovule, embryo, cotyledon,hypocotyl, meristematic cell, protoplast, root, root tip, pistil,anther, flower, shoot, stem, pod and petiole.

In another aspect, the present disclosure includes a tissue cultureproduced from a protoplast or cell from a tobacco plant, or partthereof, produced by growing the seed of tobacco cultivar NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121058, where the cell orprotoplast of the tissue culture is produced from a plant part selectedfrom the group consisting of a leaf, pollen, embryo, cotyledon,hypocotyl, meristematic cell, root, root tip, pistil, anther, flower,shoot, stem, pod, and petiole.

In an aspect, the present disclosure includes a tobacco plantregenerated from a tissue culture produced from a protoplast or cellfrom a tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121058, where the cell or protoplast of the tissue culture isproduced from a plant part selected from the group consisting of a leaf,pollen, embryo, cotyledon, hypocotyl, meristematic cell, root, root tip,pistil, anther, flower, shoot, stem, pod, and petiole where theregenerated plant has all, or essentially all of the morphological andphysiological characteristics of cultivar NC 2002 SRC.

In an aspect, the present disclosure includes an F₁ progeny plant oftobacco cultivar NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121058.

In another aspect, the present disclosure includes an F₁ progeny plantof tobacco cultivar NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121058, where the F₁ plant is cytoplasmic male sterile (CMS).

In another aspect, the present disclosure includes an F₁ progeny seedproduced by crossing two tobacco plants and harvesting the resultanttobacco seed, where at least one tobacco plant is a tobacco plantproduced by growing the seed of tobacco cultivar NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121058, where at least one tobaccoplant is cytoplasmic male sterile (CMS).

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar NC2002 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121058.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar NC2002 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121058, where theplant of tobacco cultivar NC 2002 SRC is the male parent.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar NC2002 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121058, where theplant of tobacco cultivar NC 2002 SRC is the female parent.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar NC2002 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121058, where atleast one tobacco plant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure also includes a container of F₁progeny seeds produced by crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is a tobaccoplant produced by growing the seed of tobacco cultivar NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121058, where at least one tobaccoplant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure includes an F₁ progeny plantproduced by growing a seed produced by crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar NC2002 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121058, and whereat least one tobacco plant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is a tobacco plant produced by growing the seed oftobacco cultivar NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121058, where at least one tobacco plant is cytoplasmic male sterile(CMS).

In another aspect, the present disclosure further includes a harvestedleaf of an F₁ progeny plant having a reduced amount of nornicotineand/or N′-nitrosonornicotine (NNN) produced by growing a seed producedby a method of comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is a tobaccoplant produced by growing the seed of tobacco cultivar NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121058, where at least one tobaccoplant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant having a reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN) in a smoke stream produced from the leaf,where the plant is produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is a tobacco plant produced by growing the seed oftobacco cultivar NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121058, where at least one tobacco plant is cytoplasmic male sterile(CMS).

In an aspect, the present disclosure includes a tobacco product preparedfrom an F₁ progeny tobacco plant, or part thereof, where the plant orpart thereof is produced by growing an F₁ progeny seed produced by amethod comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is produced bygrowing a seed of tobacco cultivar NC 2002 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121058, where at least one tobacco plant iscytoplasmic male sterile, and where the tobacco product is selected fromthe group consisting of pipe tobacco, cigar tobacco, cigarette tobacco,chewing tobacco, leaf tobacco, shredded tobacco, cut tobacco, and anycombination thereof.

In an aspect, the present disclosure further includes a tobacco productprepared from an F₁ progeny tobacco plant, or part thereof, where theplant or part thereof is produced by growing an F₁ progeny seed producedby a method comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is produced bygrowing a seed of tobacco cultivar NC 2002 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121058, where at least one tobacco plant iscytoplasmic male sterile, and where the tobacco product is selected fromthe group consisting of a cigarillo, a non-ventilated recess filtercigarette, a vented recess filter cigarette, a cigar, snuff, and chewingtobacco.

In another aspect, the present disclosure includes a seed of tobaccocultivar CMS NC 2002 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121041.

In an aspect, the present disclosure includes a tobacco plant, or partthereof, produced by growing the seed of tobacco cultivar CMS NC 2002SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121041.

In another aspect, the present disclosure includes a harvested leaf, orpart thereof, of a tobacco plant, or part thereof, produced by growingthe seed of tobacco cultivar CMS NC 2002 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121041.

In an aspect, the present disclosure includes a harvested leaf, or partthereof, of a tobacco plant, produced by growing the seed of tobaccocultivar CMS NC 2002 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121041,where the leaf has a reduced amount of nornicotine and/orN-nitrosonornicotine (NNN).

In another aspect, the present disclosure includes a harvested leaf, orpart thereof, produced by growing the seed of tobacco cultivar CMS NC2002 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121041, where theleaf has a reduced amount of nornicotine and/or N-nitrosonornicotine(NNN), where the reduced amount of nornicotine and/orN-nitrosonomicotine (NNN) is reduced in a smoke stream produced from theleaf.

In a further aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar CMS NC 2002 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121041, where the product is selected from the group consistingof pipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof.

In an aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar CMS NC 2002 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121041, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco.

In another aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar CMS NC 2002 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121041, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco where theproduct has a reduced amount of nornicotine and/or N′-nitrosonornicotine(NNN).

In a further aspect, the present disclosure includes a second tobaccoproduct prepared or produced from a first tobacco product prepared froma tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar CMS NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121041, where first product is selected from the group consisting ofpipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof, andthe second tobacco product is selected from the group consisting of acigarillo, a non-ventilated recess filter cigarette, a vented recessfilter cigarette, a cigar, snuff, and chewing tobacco. In some aspects,the first and/or the second product comprises a reduced amount ofnornicotine and/or N′-nitrosonornicotine (NNN).

In an aspect, the present disclosure includes a part of a tobacco plant,produced by growing the seed of tobacco cultivar CMS NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121041, where the part is selectedfrom the group consisting of leaf, pollen, ovule, embryo, cotyledon,hypocotyl, meristematic cell, protoplast, root, root tip, pistil,anther, flower, shoot, stem, pod, and petiole.

In another aspect, the present disclosure includes a tissue cultureproduced from a protoplast or cell from a tobacco plant, or partthereof, produced by growing the seed of tobacco cultivar CMS NC 2002SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121041, where the cell orprotoplast of the tissue culture is produced from a plant part selectedfrom the group consisting of a leaf, pollen, embryo, cotyledon,hypocotyl, meristematic cell, root, root tip, pistil, anther, flower,shoot, stem, pod, and petiole.

In an aspect, the present disclosure includes a tobacco plantregenerated from a tissue culture produced from a protoplast or cellfrom a tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar CMS NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121041, where the cell or protoplast of the tissue culture can beproduced from a plant part selected from the group consisting of a leaf,pollen, embryo, cotyledon, hypocotyl, meristematic cell, root, root tip,pistil, anther, flower, shoot, stem, pod, and petiole where theregenerated plant has all, or essentially all of the morphological andphysiological characteristics of cultivar CMS NC 2002 SRC.

In an aspect, the present disclosure includes an F₁ progeny plant oftobacco cultivar CMS NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121041.

In another aspect, the present disclosure includes an F₁ progeny plantof tobacco cultivar CMS NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121041, where the F₁ plant is cytoplasmic male sterile (CMS).

In another aspect, the present disclosure includes an F₁ progeny seedproduced by crossing two tobacco plants and harvesting the resultanttobacco seed, where at least one tobacco plant is cytoplasmic malesterile (CMS), where the cytoplasmic male sterile plant is a plant oftobacco cultivar CMS NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121041.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar CMSNC 2002 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121041.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar CMSNC 2002 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121041, where theplant of tobacco cultivar CMS NC 2002 SRC is the female parent.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis cytoplasmic male sterile (CMS), where the cytoplasmic male sterileplant is a plant of tobacco cultivar CMS NC 2002 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121041.

In an aspect, the present disclosure also includes a container of F₁progeny seeds produced by crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is cytoplasmicmale sterile (CMS), where the cytoplasmic male sterile plant is atobacco plant produced by growing the seed of tobacco cultivar CMS NC2002 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121041.

In an aspect, the present disclosure includes an F₁ progeny plantproduced by growing a seed produced by crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis cytoplasmic male sterile (CMS), where the cytoplasmic male sterileplant is a tobacco plant produced by growing the seed of tobaccocultivar CMS NC 2002 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121041.

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is cytoplasmic male sterile (CMS), where thecytoplasmic male sterile plant is a tobacco plant produced by growingthe seed of tobacco cultivar CMS NC 2002 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121041.

In another aspect, the present disclosure further includes a harvestedleaf of an F₁ progeny plant having a reduced amount of nornicotineand/or N′-nitrosonornicotine (NNN) produced by growing a seed producedby crossing two tobacco plants and harvesting the resultant tobaccoseed, where at least one tobacco plant is cytoplasmic male sterile(CMS), where the cytoplasmic male sterile plant is a tobacco plantproduced by growing the seed of tobacco cultivar CMS NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121041.

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant having a reduced amount of nornicotine and/orN′-nitrosonomicotine (NNN) in a smoke stream produced from the leaf,where the plant is produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is cytoplasmic male sterile (CMS), where thecytoplasmic male sterile plant is a tobacco plant produced by growingthe seed of tobacco cultivar CMS NC 2002 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121041.

In an aspect, the present disclosure includes a tobacco product preparedfrom an F₁ progeny tobacco plant, or part thereof, where the plant orpart thereof is produced by growing an F₁ progeny seed produced bycrossing two tobacco plants and harvesting the resultant tobacco seed,where at least one tobacco plant is cytoplasmic male sterile (CMS),where the cytoplasmic male sterile plant is a tobacco plant produced bygrowing the seed of tobacco cultivar CMS NC 2002 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121041, and where the tobacco product is selectedfrom the group consisting of pipe tobacco, cigar tobacco, cigarettetobacco, chewing tobacco, leaf tobacco, shredded tobacco, cut tobaccoand any combination thereof.

In an aspect, the present disclosure further includes a tobacco productprepared from an F₁ progeny tobacco plant, or part thereof, where theplant or part thereof is produced by growing an F₁ progeny seed producedby crossing two tobacco plants and harvesting the resultant tobaccoseed, where at least one tobacco plant is cytoplasmic male sterile(CMS), where the cytoplasmic male sterile plant is a tobacco plantproduced by growing the seed of tobacco cultivar CMS NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121041, and where the tobaccoproduct is selected from the group consisting of a cigarillo, anon-ventilated recess filter cigarette, a vented recess filtercigarette, a cigar, snuff, and chewing tobacco.

In an aspect, the present disclosure further includes a tobacco productprepared from an F₁ progeny tobacco plant, or part thereof, where theplant or part thereof is produced by growing an F₁ progeny seed producedby crossing two tobacco plants and harvesting the resultant tobaccoseed, where at least one tobacco plant is cytoplasmic male sterile(CMS), where the cytoplasmic male sterile plant is a tobacco plantproduced by growing the seed of tobacco cultivar CMS NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121041, and where the tobaccoproduct is selected from the group consisting of a cigarillo, anon-ventilated recess filter cigarette, a vented recess filtercigarette, a cigar, snuff, and chewing tobacco, and further where theproduct has a reduced amount of nornicotine and/or N′-nitrosonornicotine(NNN).

In an aspect, the present disclosure includes a method for producing atobacco seed comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is cytoplasmicmale sterile, where the cytoplasmic male sterile plant is a plant oftobacco cultivar CMS NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121041.

In an aspect, the present disclosure includes a method of vegetativelypropagating a plant of a tobacco cultivar comprising the steps of (a)collecting tissue capable of being propagated from a plant of a tobaccocultivar NC 2002 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121058or CMS NC 2002 SRC, a representative sample seed of the cultivar havingbeen deposited with the ATCC under ATCC Accession No. PTA-121041; (b)cultivating the tissue to obtain a proliferated shoot; and (c) rootingthe proliferated shoots to obtain a rooted plantlet.

In an aspect, the present disclosure includes a method of vegetativelypropagating a plant of a tobacco cultivar comprising the steps of (a)collecting tissue capable of being propagated from a plant of a tobaccocultivar NC 2002 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121058or CMS NC 2002 SRC, a representative sample seed of the cultivar havingbeen deposited with the ATCC under ATCC Accession No. PTA-121041; (b)cultivating the tissue to obtain a proliferated shoot; (c) rooting theproliferated shoots to obtain a rooted plantlet; and (d) growing a plantfrom the rooted plantlet.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar NC 2002 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121058 or CMS NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121041, with a second tobacco plant that comprises a desired traitto produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of said firsttobacco cultivar to produce a backcross BC₁F₁ progeny seed; (d) growingthe BC₁F₁ progeny seed and selecting a backcross BC₁F₁ progeny plantcomprising the desired trait and essentially all of the physiologicaland morphological characteristics of said first tobacco cultivar, NC2002 SRC or CMS NC 2002 SRC; and (e) repeating steps (c) and (d) threeor more times (e.g., 3, 4, 5, 6, 7, 8, 9, 10, and the like) insuccession to produce selected fourth or higher backcross progeny thatcomprise the desired trait. In additional aspects, steps (c) and (d) canbe repeated one or more times (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, andthe like) in succession to produce second or higher backcross progenycomprising the desired trait.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar NC 2002 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121058, with a second tobacco plant that comprises a desiredtrait to produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of the first tobaccocultivar NC 2002 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121058to produce a backcross BC₁F₁ progeny seed; (d) growing the BC₁F₁ progenyseed and selecting a backcross BC₁F₁ progeny plant comprising thedesired trait and essentially all of the physiological and morphologicalcharacteristics of the first tobacco cultivar, NC 2002 SRC; and (e)repeating steps (c) and (d) three or more times in succession to produceselected fourth or higher backcross progeny that comprise the desiredtrait, where the trait is cytoplasmic male sterility (CMS). Inadditional aspects, steps (c) and (d) can be repeated one or more timesin succession to produce second or higher backcross progeny comprisingthe desired trait, where the trait is CMS.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar NC 2002 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121058, with a second tobacco plant that comprises a desiredtrait to produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of the first tobaccocultivar NC 2002 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121058to produce a backcross BC₁F₁ progeny seed; (d) growing the BC₁F₁ progenyseed and selecting a backcross BC₁F₁ progeny plant comprising thedesired trait and the physiological and essentially all of morphologicalcharacteristics of the first tobacco cultivar NC 2002 SRC; and (e)repeating steps (c) and (d) three or more times in succession to produceselected fourth or higher backcross progeny that comprise the desiredtrait, where the trait is cytoplasmic male sterility (CMS) and the CMStrait is obtained from the cytoplasm of Nicotiana suaveolens orNicotiana glauca. In additional aspects, steps (c) and (d) can berepeated one or more times in succession to produce second or higherbackcross progeny comprising the desired trait, where the trait is CMSand the CMS trait is obtained from the cytoplasm of Nicotiana suaveolensor Nicotiana glauca.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar NC 2002 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121058 with a second tobacco plant that comprises a desiredtrait to produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of the first tobaccocultivar, NC 2002 SRC to produce a backcross BC₁F₁ progeny seed; (d)growing the BC₁F₁ progeny seed and selecting a backcross BC₁F₁ progenyplant comprising the desired trait and essentially all of thephysiological and morphological characteristics of tobacco cultivar NC2002 SRC; and (e) repeating steps (c) and (d) three or more times insuccession to produce selected fourth or higher backcross progeny thatcomprise the desired trait, where the trait is cytoplasmic malesterility (CMS) and the CMS trait is obtained from the cytoplasm ofNicotiana suaveolens, and where the second tobacco plant is CMS NC 2002SRC. In additional aspects, steps (c) and (d) can be repeated one ormore times in succession to produce second or higher backcross progenycomprising the desired trait, where the trait is CMS and the CMS traitis obtained from the cytoplasm of Nicotiana suaveolens.

In an aspect, the present disclosure includes a tobacco plant producedby a method comprising introducing a desired trait into a tobaccocultivar comprising: (a) crossing a plant of a first tobacco cultivar NC2002 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121058 or CMS NC2002 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121041, with asecond tobacco plant that comprises a desired trait to produce an F₁progeny seed; (b) growing the F₁ progeny seed and selecting an F₁progeny plant that comprises the desired trait; (c) crossing theselected F₁ progeny plant with a plant of the first tobacco cultivar NC2002 SRC or CMS NC 2002 SRC to produce a backcross BC₁F₁ progeny seed;(d) growing the BC₁F₁ progeny seed and selecting a backcross BC₁F₁progeny plant comprising the desired trait and essentially all of thephysiological and morphological characteristics of the first tobaccocultivar NC 2002 SRC or CMS NC 2002 SRC; and (e) repeating steps (c) and(d) three or more times in succession to produce selected fourth orhigher backcross progeny that comprise the desired trait. In additionalaspects, steps (c) and (d) can be repeated one or more times insuccession to produce second or higher backcross progeny that comprisethe desired trait.

In another aspect, the present disclosure includes a method ofintroducing a desired trait into a tobacco cultivar comprising: (a)crossing a plant of a first tobacco cultivar NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121058 or CMS NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121041 with a plant of a secondtobacco cultivar that comprises a desired trait to produce a progenyplant where the desired trait is selected from the group consisting ofherbicide resistance, pest resistance, disease resistance, high yield,high grade index, curability, curing quality, mechanical harvestability,holding ability, leaf quality, height, plant maturation, early maturing,early to medium maturing, medium maturing, medium to late maturing, latematuring, small stalk, medium stalk, large stalk, leaf number per plant,5-10 leaves per plant, 11-15 leaves per plant, 16-21 leaves per plant,and any combination thereof, to produce an F₁ progeny seed; (b) growingthe F₁ progeny seed into an F₁ progeny plant and selecting the F₁progeny plant having the desired trait; (c) crossing the selected F₁progeny plant with a plant of the first tobacco cultivar NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121058 or CMS NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121041 to produce a backcrossprogeny plant seed; (d) growing the backcross progeny plant seed into abackcross progeny plant and selecting the backcross progeny plantcomprising the desired trait and essentially all of the physiologicaland morphological characteristics of the first tobacco cultivar, NC 2002SRC or CMS NC 2002 SRC; and (e) repeating steps (c) and (d) one or moretimes in succession to produce a selected second, third, fourth orhigher backcross progeny plant that comprises the desired trait andessentially all of the physiological and morphological characteristicsof the first tobacco cultivar, NC 2002 SRC or CMS NC 2002 SRC.

In another aspect, the present disclosure includes a method ofintroducing a desired trait into a tobacco cultivar comprising: (a)crossing a plant of a first tobacco cultivar NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121058 or CMS NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121041, with a plant of a secondtobacco cultivar that comprises a desired trait to produce a progenyplant where the desired trait is selected from the group consisting ofherbicide resistance, pest resistance, disease resistance, high yield,high grade index, curability, curing quality, mechanical harvestability,holding ability, leaf quality, height, plant maturation, early maturing,early to medium maturing, medium maturing, medium to late maturing, latematuring, small stalk, medium stalk, large stalk, leaf number per plant,5-10 leaves per plant, 11-15 leaves per plant, 16-21 leaves per plant,and any combination thereof, to produce an F₁ progeny seed; (b) growingthe F₁ progeny seed into an F₁ progeny plant and selecting the F₁progeny plant having the desired trait; (c) crossing the selected F₁progeny plant with a plant of the first tobacco cultivar to produce abackcross progeny plant seed; (d) growing the backcross progeny plantseed into a backcross progeny plant and selecting the backcross progenyplant comprising the desired trait and essentially all of thephysiological and morphological characteristics of the first tobaccocultivar, NC 2002 SRC or CMS NC 2002 SRC; and (e) repeating steps (c)and (d) one or more times in succession to produce a selected second,third, fourth or higher backcross progeny plant that comprises thedesired trait and essentially all of the physiological and morphologicalcharacteristics of the first tobacco cultivar, NC 2002 SRC or CMS NC2002 SRC.

In another aspect, the present disclosure includes a method ofintroducing a desired trait into a tobacco cultivar comprising: (a)crossing a plant of a first tobacco cultivar NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121058 or CMS NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121041, with a plant of a secondtobacco cultivar that comprises a desired trait to produce a progenyplant where the desired trait is selected from the group consisting ofherbicide resistance, pest resistance, disease resistance, high yield,high grade index, curability, curing quality, mechanical harvestability,holding ability, leaf quality, height, plant maturation, early maturing,early to medium maturing, medium maturing, medium to late maturing, latematuring, small stalk, medium stalk, large stalk, leaf number per plant,5-10 leaves per plant, 11-15 leaves per plant, 16-21 leaves per plant,and any combination thereof, to produce an F₁ progeny seed; (b) growingthe F₁ progeny seed into an F₁ progeny plant and selecting the F₁progeny plant having the desired trait; (c) crossing the selected F₁progeny plant with a plant of the first tobacco cultivar to produce abackcross progeny plant seed; (d) growing the backcross progeny plantseed into a backcross progeny plant and selecting the backcross progenyplant comprising the desired trait and essentially all of thephysiological and morphological characteristics of the first tobaccocultivar, NC 2002 SRC or CMS NC 2002 SRC; and (e) repeating steps (c)and (d) one or more times in succession to produce a selected second,third, fourth or higher backcross progeny plant that comprises thedesired trait and essentially all of the physiological and morphologicalcharacteristics of the first tobacco cultivar, NC 2002 SRC or CMS NC2002 SRC, where the plant has a desired trait of disease resistance.

In an aspect, the present disclosure includes a method for producing atobacco plant having decreased nicotine conversion comprising:identifying a first tobacco plant comprising a nucleotide sequenceselected from the group consisting of the nucleotide sequence of SEQ IDNO: 1, SEQ ID NO: 2, SEQ ID NO: 11, and any combination thereof;crossing the first tobacco plant with a second tobacco plant andcollecting an F₁ seed; crossing a plant grown from the F₁ seed to athird tobacco plant and collecting a second tobacco seed; andidentifying a second tobacco seed or a plant grown from the second seedthat is homozygous for the nucleotide sequence selected from the groupconsisting of the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 11, and any combination thereof, where the first tobacco plant isa plant of tobacco cultivar NC 2002 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121058, or tobacco cultivar CMS NC 2002 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121041.

In an aspect, the present disclosure includes a method for producing atobacco plant having decreased nicotine conversion comprising:identifying a first tobacco plant comprising a nucleotide sequenceselected from the group consisting of the nucleotide sequence of SEQ IDNO: 1, SEQ ID NO: 2, SEQ ID NO: 11, and any combination thereof;crossing the first tobacco plant with a second tobacco plant andcollecting an F₁ seed; crossing a plant grown from the F₁ seed to athird tobacco plant and collecting a second tobacco seed; andidentifying a second tobacco seed or a plant grown from the second seedthat is homozygous for the nucleotide sequence selected from the groupconsisting of the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 11, and any combination thereof, where the third tobacco plant isa plant of tobacco cultivar NC 2002 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121058, or tobacco cultivar CMS NC 2002 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121041.

In another aspect, the present disclosure includes a method of producinga plant of a tobacco cultivar NC 2002 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121058 or tobacco cultivar CMS NC 2002 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121041 comprising at least one (e.g., 1, 2, 3, 4,5, 6, 7, 8, and the like) additional desired trait comprising the stepsof: (a) collecting tissue capable of being propagated from a plant of atobacco cultivar NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121058 or CMS NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121041; and (b) introducing a at least one transgene (nucleic acidconstruct) conferring at least one desired trait into the tissue.

In another aspect, the present disclosure includes a method of producingan herbicide resistant tobacco plant comprising transforming a tobaccoplant, or part thereof, produced by growing a seed of tobacco cultivarNC 2002 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121058 or tobaccocultivar CMS NC 2002 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121041with at least one transgene (nucleic acid construct), where the at leastone transgene (nucleic acid construct) confers resistance to anherbicide selected from the group consisting of imidazolinone,cyclohexanedione, sulfonylurea, glyphosate, glufosinate, phenoxyproprionic acid, L-phosphinothricin, triazine, benzonitrile, and anycombination thereof.

In another aspect, the present disclosure includes an herbicideresistant tobacco plant produced by a method comprising transforming atobacco plant, or part thereof; produced by growing a seed of a tobaccocultivar NC 2002 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121058or CMS NC 2002 SRC, a representative sample seed of the cultivar havingbeen deposited with the ATCC under ATCC Accession No. PTA-121041 with atleast one transgene (nucleic acid construct), where the at least onetransgene (nucleic acid construct) confers resistance to an herbicideselected from the group consisting of imidazolinone, cyclohexanedione,sulfonylurea, glyphosate, glufosinate, phenoxy proprionic acid,L-phosphinothricin, triazine, benzonitrile, and any combination thereof.

In another aspect, the present disclosure includes a method of producinga pest and/or insect resistant tobacco plant where the method comprisestransforming a tobacco plant produced by growing a seed of tobaccocultivar NC 2002 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121058or tobacco cultivar CMS NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121041, with at least one transgene (nucleic acid construct) thatconfers pest and/or insect resistance.

In a further aspect, the present disclosure includes a pest and/orinsect resistant tobacco plant produced by a method comprisingtransforming a tobacco plant produced by growing a seed of tobaccocultivar NC 2002 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121058or tobacco cultivar CMS NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121041, with at least one transgene (nucleic acid construct) thatconfers pest and/or insect resistance.

In a further aspect, the present disclosure includes a pest and/orinsect resistant tobacco plant produced by a method comprisingtransforming a tobacco plant produced by growing a seed of tobaccocultivar NC 2002 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121058or tobacco cultivar CMS NC 2002 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121041, with at least one transgene (nucleic acid construct) thatconfers pest and/or insect resistance, where the transgene (nucleic acidconstruct) encodes a Bacillus thuringiensis (BT) endotoxin.

In another aspect, the present disclosure includes a method of producinga disease resistant tobacco plant, the method comprising transforming atobacco plant produced by growing a seed of tobacco cultivar NC 2002SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121058 or tobacco cultivarCMS NC 2002 SRC, a representative sample seed of the cultivar havingbeen deposited with the ATCC under ATCC Accession No. PTA-121041 with atleast one transgene (nucleic acid construct) that confers diseaseresistance.

In a further aspect, the present disclosure includes a disease resistanttobacco plant produced by transforming a tobacco plant produced bygrowing a seed of tobacco cultivar NC 2002 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121058 or tobacco cultivar CMS NC 2002 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121041 with at least one transgene(nucleic acid construct) that confers disease resistance.

In an aspect, the present disclosure includes a seed of tobacco cultivarBurley 21 SRC, a representative sample seed of the cultivar having beendeposited with the American Type Culture Collection (ATCC) under ATCCAccession No. PTA-121059.

In another aspect, the present disclosure includes a tobacco plant, orpart thereof, produced by growing the seed of tobacco cultivar Burley 21SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121059.

In a further aspect, the present disclosure includes a harvested leaf,or part thereof, of a tobacco plant, or part thereof, produced bygrowing the seed of tobacco cultivar Burley 21 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121059.

In an aspect, the present disclosure includes a harvested leaf, or partthereof, of a tobacco plant, produced by growing the seed of tobaccocultivar Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121059,where the leaf has a reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN).

In an aspect, the present disclosure includes a harvested leaf, or partthereof, produced by growing the seed of tobacco cultivar Burley 21 SRC,a representative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121059, where the leaf has areduced amount of nornicotine and/or N′-nitrosonornicotine (NNN), wherethe reduced amount of nornicotine and/or N′-nitrosonornicotine (NNN) isreduced in a smoke stream produced from the leaf.

In a further aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar Burley 21 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121059, where the product is selected from the group consistingof pipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof.

In an aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar Burley 21 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121059, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco.

In another aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar Burley 21 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121059, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco, and wherethe product has a reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN).

In a further aspect, the present disclosure includes a second tobaccoproduct prepared or produced from a first tobacco product prepared froma tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar Burley 21 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121059, where first product is selected from the group consisting ofpipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof, andthe second tobacco product is selected from the group consisting of acigarillo, a non-ventilated recess filter cigarette, a vented recessfilter cigarette, a cigar, snuff, and chewing tobacco. In some aspects,the first and second product comprises a reduced amount of nornicotineand/or N′-nitrosonornicotine (NNN).

In an aspect, the present disclosure includes a part of a tobacco plant,produced by growing the seed of tobacco cultivar Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121059, where the part is selectedfrom the group consisting of leaf, pollen, ovule, embryo, cotyledon,hypocotyl, meristematic cell, protoplast, root, root tip, pistil,anther, flower, shoot, stem, pod and petiole.

In another aspect, the present disclosure includes a tissue cultureproduced from a protoplast or cell from a tobacco plant, or partthereof, produced by growing the seed of tobacco cultivar Burley 21 SRC,a representative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121059, where the cell orprotoplast of the tissue culture is produced from a plant part selectedfrom the group consisting of a leaf, pollen, embryo, cotyledon,hypocotyl, meristematic cell, root, root tip, pistil, anther, flower,shoot, stem, pod, and petiole.

In an aspect, the present disclosure includes a tobacco plantregenerated from a tissue culture produced from a protoplast or cellfrom a tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar Burley 21 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121059, where the cell or protoplast of the tissue culture isproduced from a plant part selected from the group consisting of a leaf,pollen, embryo, cotyledon, hypocotyl, meristematic cell, root, root tip,pistil, anther, flower, shoot, stem, pod, and petiole where theregenerated plant has all, or essentially all of the morphological andphysiological characteristics of cultivar Burley 21 SRC.

In an aspect, the present disclosure includes an F₁ progeny plant oftobacco cultivar Burley 21 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121059.

In another aspect, the present disclosure includes an F₁ progeny plantof tobacco cultivar Burley 21 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121059, where the F₁ plant is cytoplasmic male sterile (CMS).

In another aspect, the present disclosure includes an F₁ progeny seedproduced by crossing two tobacco plants and harvesting the resultanttobacco seed, where at least one tobacco plant is a tobacco plantproduced by growing the seed of tobacco cultivar Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121059, where at least one tobaccoplant is cytoplasmic male sterile (CMS).

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivarBurley 21 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121059.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivarBurley 21 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121059, where theplant of tobacco cultivar Burley 21 SRC is the male parent.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivarBurley 21 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121059, where theplant of tobacco cultivar Burley 21 SRC is the female parent.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivarBurley 21 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121059, where atleast one tobacco plant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure also includes a container of F₁progeny seeds produced by crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is a tobaccoplant produced by growing the seed of tobacco cultivar Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121059, where at least one tobaccoplant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure includes an F₁ progeny plantproduced by growing a seed produced by crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivarBurley 21 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121059, and whereat least one tobacco plant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is a tobacco plant produced by growing the seed oftobacco cultivar Burley 21 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121059, where at least one tobacco plant is cytoplasmic male sterile(CMS).

In another aspect, the present disclosure further includes a harvestedleaf of an F₁ progeny plant having a reduced amount of nornicotineand/or N′-nitrosonornicotine (NNN) produced by growing a seed producedby a method of comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is a tobaccoplant produced by growing the seed of tobacco cultivar Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121059, where at least one tobaccoplant is cytoplasmic male sterile (CMS).

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant having a reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN) in a smoke stream produced from the leaf,where the plant is produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is a tobacco plant produced by growing the seed oftobacco cultivar Burley 21 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121059, where at least one tobacco plant is cytoplasmic male sterile(CMS).

In an aspect, the present disclosure includes a tobacco product preparedfrom an F₁ progeny tobacco plant, or part thereof, where the plant orpart thereof is produced by growing an F₁ progeny seed produced by amethod comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is produced bygrowing a seed of tobacco cultivar Burley 21 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121059, where at least one tobacco plant iscytoplasmic male sterile, and where the tobacco product is selected fromthe group consisting of pipe tobacco, cigar tobacco, cigarette tobacco,chewing tobacco, leaf tobacco, shredded tobacco, cut tobacco, and anycombination thereof.

In an aspect, the present disclosure further includes a tobacco productprepared from an F₁ progeny tobacco plant, or part thereof, where theplant or part thereof is produced by growing an F₁ progeny seed producedby a method comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is produced bygrowing a seed of tobacco cultivar Burley 21 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121059, where at least one tobacco plant iscytoplasmic male sterile, and where the tobacco product is selected fromthe group consisting of a cigarillo, a non-ventilated recess filtercigarette, a vented recess filter cigarette, a cigar, snuff, and chewingtobacco.

In another aspect, the present disclosure includes a seed of tobaccocultivar CMS Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121044.

In an aspect, the present disclosure includes a tobacco plant, or partthereof, produced by growing the seed of tobacco cultivar CMS Burley 21SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121044.

In another aspect, the present disclosure includes a harvested leaf, orpart thereof, of a tobacco plant, or part thereof, produced by growingthe seed of tobacco cultivar CMS Burley 21 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121044.

In an aspect, the present disclosure includes a harvested leaf, or partthereof, of a tobacco plant, produced by growing the seed of tobaccocultivar CMS Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121044,where the leaf has a reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN).

In another aspect, the present disclosure includes a harvested leaf, orpart thereof, produced by growing the seed of tobacco cultivar CMSBurley 21 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121044, where theleaf has a reduced amount of nornicotine and/or N′-nitrosonornicotine(NNN), where the reduced amount of nornicotine and/orN′-nitrosonornicotine (NNN) is reduced in a smoke stream produced fromthe leaf.

In a further aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar CMS Burley 21 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121044, where the product is selected from the group consistingof pipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof.

In an aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar CMS Burley 21 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121044, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco.

In another aspect, the present disclosure includes a tobacco product,prepared from a tobacco plant, or part thereof, produced by growing theseed of tobacco cultivar CMS Burley 21 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121044, where the product is selected from the group consistingof a cigarillo, a non-ventilated recess filter cigarette, a ventedrecess filter cigarette, a cigar, snuff, and chewing tobacco where theproduct has a reduced amount of nornicotine and/or N′-nitrosonornicotine(NNN).

In a further aspect, the present disclosure includes a second tobaccoproduct prepared or produced from a first tobacco product prepared froma tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar CMS Burley 21 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121044, where first product is selected from the group consisting ofpipe tobacco, cigar tobacco, cigarette tobacco, chewing tobacco, leaftobacco, shredded tobacco, cut tobacco and any combination thereof, andthe second tobacco product is selected from the group consisting of acigarillo, a non-ventilated recess filter cigarette, a vented recessfilter cigarette, a cigar, snuff, and chewing tobacco. In some aspects,the first and/or the second product comprises a reduced amount ofnornicotine and/or N′-nitrosonornicotine (NNN).

In an aspect, the present disclosure includes a part of a tobacco plant,produced by growing the seed of tobacco cultivar CMS Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121044, where the part is selectedfrom the group consisting of leaf, pollen, ovule, embryo, cotyledon,hypocotyl, meristematic cell, protoplast, root, root tip, pistil,anther, flower, shoot, stem, pod, and petiole.

In another aspect, the present disclosure includes a tissue cultureproduced from a protoplast or cell from a tobacco plant, or partthereof, produced by growing the seed of tobacco cultivar CMS Burley 21SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121044, where the cell orprotoplast of the tissue culture is produced from a plant part selectedfrom the group consisting of a leaf, pollen, embryo, cotyledon,hypocotyl, meristematic cell, root, root tip, pistil, anther, flower,shoot, stem, pod, and petiole.

In an aspect, the present disclosure includes a tobacco plantregenerated from a tissue culture produced from a protoplast or cellfrom a tobacco plant, or part thereof, produced by growing the seed oftobacco cultivar CMS Burley 21 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121044, where the cell or protoplast of the tissue culture can beproduced from a plant part selected from the group consisting of a leaf,pollen, embryo, cotyledon, hypocotyl, meristematic cell, root, root tip,pistil, anther, flower, shoot, stem, pod, and petiole where theregenerated plant has all, or essentially all of the morphological andphysiological characteristics of cultivar CMS Burley 21 SRC.

In an aspect, the present disclosure includes an F₁ progeny plant oftobacco cultivar CMS Burley 21 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121044.

In another aspect, the present disclosure includes an F₁ progeny plantof tobacco cultivar CMS Burley 21 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121044, where the F₁ plant is cytoplasmic male sterile (CMS).

In another aspect, the present disclosure includes an F₁ progeny seedproduced by crossing two tobacco plants and harvesting the resultanttobacco seed, where at least one tobacco plant is cytoplasmic malesterile (CMS), where the cytoplasmic male sterile plant is a plant oftobacco cultivar CMS Burley 21 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121044.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar CMSBurley 21 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121044.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis a tobacco plant produced by growing the seed of tobacco cultivar CMSBurley 21 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121044, where theplant of tobacco cultivar CMS Burley 21 SRC is the female parent.

In another aspect, the present disclosure includes a method forproducing a tobacco seed comprising crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis cytoplasmic male sterile (CMS), where the cytoplasmic male sterileplant is a plant of tobacco cultivar CMS Burley 21 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121044.

In an aspect, the present disclosure also includes a container of F₁progeny seeds produced by crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is cytoplasmicmale sterile (CMS), where the cytoplasmic male sterile plant is atobacco plant produced by growing the seed of tobacco cultivar CMSBurley 21 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121044.

In an aspect, the present disclosure includes an F₁ progeny plantproduced by growing a seed produced by crossing two tobacco plants andharvesting the resultant tobacco seed, where at least one tobacco plantis cytoplasmic male sterile (CMS), where the cytoplasmic male sterileplant is a tobacco plant produced by growing the seed of tobaccocultivar CMS Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121044.

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is cytoplasmic male sterile (CMS), where thecytoplasmic male sterile plant is a tobacco plant produced by growingthe seed of tobacco cultivar CMS Burley 21 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121044.

In another aspect, the present disclosure further includes a harvestedleaf of an F₁ progeny plant having a reduced amount of nornicotineand/or N′-nitrosonornicotine (NNN) produced by growing a seed producedby crossing two tobacco plants and harvesting the resultant tobaccoseed, where at least one tobacco plant is cytoplasmic male sterile(CMS), where the cytoplasmic male sterile plant is a tobacco plantproduced by growing the seed of tobacco cultivar CMS Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121044.

In an aspect, the present disclosure also includes a harvested leaf ofan F₁ progeny plant having a reduced amount of nornicotine and/orN′-nitrosonomicotine (NNN) in a smoke stream produced from the leaf,where the plant is produced by growing a seed produced by crossing twotobacco plants and harvesting the resultant tobacco seed, where at leastone tobacco plant is cytoplasmic male sterile (CMS), where thecytoplasmic male sterile plant is a tobacco plant produced by growingthe seed of tobacco cultivar CMS Burley 21 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121044.

In an aspect, the present disclosure includes a tobacco product preparedfrom an F₁ progeny tobacco plant, or part thereof, where the plant orpart thereof is produced by growing an F₁ progeny seed produced bycrossing two tobacco plants and harvesting the resultant tobacco seed,where at least one tobacco plant is cytoplasmic male sterile (CMS),where the cytoplasmic male sterile plant is a tobacco plant produced bygrowing the seed of tobacco cultivar CMS Burley 21 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121044, and where the tobacco product is selectedfrom the group consisting of pipe tobacco, cigar tobacco, cigarettetobacco, chewing tobacco, leaf tobacco, shredded tobacco, cut tobaccoand any combination thereof.

In an aspect, the present disclosure further includes a tobacco productprepared from an F₁ progeny tobacco plant, or part thereof, where theplant or part thereof is produced by growing an F₁ progeny seed producedby crossing two tobacco plants and harvesting the resultant tobaccoseed, where at least one tobacco plant is cytoplasmic male sterile(CMS), where the cytoplasmic male sterile plant is a tobacco plantproduced by growing the seed of tobacco cultivar CMS Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121044, and where the tobaccoproduct is selected from the group consisting of a cigarillo, anon-ventilated recess filter cigarette, a vented recess filtercigarette, a cigar, snuff, and chewing tobacco.

In an aspect, the present disclosure further includes a tobacco productprepared from an F₁ progeny tobacco plant, or part thereof, where theplant or part thereof is produced by growing an F₁ progeny seed producedby crossing two tobacco plants and harvesting the resultant tobaccoseed, where at least one tobacco plant is cytoplasmic male sterile(CMS), where the cytoplasmic male sterile plant is a tobacco plantproduced by growing the seed of tobacco cultivar CMS Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121044, and where the tobaccoproduct is selected from the group consisting of a cigarillo, anon-ventilated recess filter cigarette, a vented recess filtercigarette, a cigar, snuff, and chewing tobacco, and further where theproduct has a reduced amount of nornicotine and/or N′-nitrosonomicotine(NNN).

In an aspect, the present disclosure further includes a method ofproducing a tobacco product, comprising: (a) providing cured tobaccofrom a tobacco plant of a cultivar or a hybrid produced from saidcultivar, wherein said cultivar is selected from the group consisting ofBanket A1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002SRC, CMS NC 2002 SRC, Burley 21 SRC, and CMS Burley 21 SRC; and (b)preparing a tobacco product from said cured tobacco, whereinrepresentative sample seeds of said Banket A1 SRC, CMS Banket A1 SRC, NC2000 SRC, CMS NC 2000 SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley 21 SRC,and CMS Burley 21 SRC have been deposited at the ATCC with the followingATCC Accession Nos.: PTA-121043 for Banket A1 SRC, PTA-121054 for CMSBanket A1 SRC, PTA-121053 for NC 2000 SRC, PTA-121045 for CMS NC 2000SRC, PTA-121058 for NC 2002 SRC, PTA-121041 for CMS NC 2002 SRC,PTA-121059 for Burley 21 SRC, and PTA-121044 for CMS Burley 21 SRC.

In an aspect, the present disclosure includes a method for producing atobacco seed comprising crossing two tobacco plants and harvesting theresultant tobacco seed, where at least one tobacco plant is cytoplasmicmale sterile, where the cytoplasmic male sterile plant is a plant oftobacco cultivar CMS Burley 21 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121044.

In an aspect, the present disclosure includes a method of vegetativelypropagating a plant of a tobacco cultivar comprising the steps of (a)collecting tissue capable of being propagated from a plant of a tobaccocultivar Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121059or CMS Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121044;(b) cultivating the tissue to obtain a proliferated shoot; and (c)rooting the proliferated shoots to obtain a rooted plantlet.

In an aspect, the present disclosure includes a method of vegetativelypropagating a plant of a tobacco cultivar comprising the steps of (a)collecting tissue capable of being propagated from a plant of a tobaccocultivar Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121059or CMS Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121044;(b) cultivating the tissue to obtain a proliferated shoot; (c) rootingthe proliferated shoots to obtain a rooted plantlet; and (d) growing aplant from the rooted plantlet.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar Burley 21 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121059 or CMS Burley 21 SRC, a representative sample seed of thecultivar having been deposited with the ATCC under ATCC Accession No.PTA-121044, with a second tobacco plant that comprises a desired traitto produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of said firsttobacco cultivar to produce a backcross BC₁F₁ progeny seed; (d) growingthe BC₁F₁ progeny seed and selecting a backcross BC₁F₁ progeny plantcomprising the desired trait and essentially all of the physiologicaland morphological characteristics of said first tobacco cultivar, Burley21 SRC or CMS Burley 21 SRC; and (e) repeating steps (c) and (d) threeor more times (e.g., 3, 4, 5, 6, 7, 8, 9, 10, and the like) insuccession to produce selected fourth or higher backcross progeny thatcomprise the desired trait. In additional aspects, steps (c) and (d) canbe repeated one or more times (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, andthe like) in succession to produce second or higher backcross progenycomprising the desired trait.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar Burley 21 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121059, with a second tobacco plant that comprises a desiredtrait to produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of the first tobaccocultivar Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121059to produce a backcross BC₁F₁ progeny seed; (d) growing the BC₁F₁ progenyseed and selecting a backcross BC₁F₁ progeny plant comprising thedesired trait and essentially all of the physiological and morphologicalcharacteristics of the first tobacco cultivar, Burley 21 SRC; and (e)repeating steps (c) and (d) three or more times in succession to produceselected fourth or higher backcross progeny that comprise the desiredtrait, where the trait is cytoplasmic male sterility (CMS). Inadditional aspects, steps (c) and (d) can be repeated one or more timesin succession to produce second or higher backcross progeny comprisingthe desired trait, where the trait is CMS.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar Burley 21 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121059, with a second tobacco plant that comprises a desiredtrait to produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of the first tobaccocultivar Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121059to produce a backcross BC₁F₁ progeny seed; (d) growing the BC₁F₁ progenyseed and selecting a backcross BC₁F₁ progeny plant comprising thedesired trait and the physiological and essentially all of morphologicalcharacteristics of the first tobacco cultivar Burley 21 SRC; and (e)repeating steps (c) and (d) three or more times in succession to produceselected fourth or higher backcross progeny that comprise the desiredtrait, where the trait is cytoplasmic male sterility (CMS) and the CMStrait is obtained from the cytoplasm of Nicotiana suaveolens orNicotiana glauca. In additional aspects, steps (c) and (d) can berepeated one or more times in succession to produce second or higherbackcross progeny comprising the desired trait, where the trait is CMSand the CMS trait is obtained from the cytoplasm of Nicotiana suaveolensor Nicotiana glauca.

In an aspect, the present disclosure includes a method of introducing adesired trait into a tobacco cultivar comprising: (a) crossing a plantof a first tobacco cultivar Burley 21 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121059 with a second tobacco plant that comprises a desiredtrait to produce an F₁ progeny seed; (b) growing the F₁ progeny seed andselecting an F₁ progeny plant that comprises the desired trait; (c)crossing the selected F₁ progeny plant with a plant of the first tobaccocultivar, Burley 21 SRC to produce a backcross BC₁F₁ progeny seed; (d)growing the BC₁F₁ progeny seed and selecting a backcross BC₁F₁ progenyplant comprising the desired trait and essentially all of thephysiological and morphological characteristics of tobacco cultivarBurley 21 SRC; and (e) repeating steps (c) and (d) three or more timesin succession to produce selected fourth or higher backcross progenythat comprise the desired trait, where the trait is cytoplasmic malesterility (CMS) and the CMS trait is obtained from the cytoplasm ofNicotiana suaveolens, and where the second tobacco plant is CMS Burley21 SRC. In additional aspects, steps (c) and (d) can be repeated one ormore times in succession to produce second or higher backcross progenycomprising the desired trait, where the trait is CMS and the CMS traitis obtained from the cytoplasm of Nicotiana suaveolens.

In an aspect, the present disclosure includes a tobacco plant producedby a method comprising introducing a desired trait into a tobaccocultivar comprising: (a) crossing a plant of a first tobacco cultivarBurley 21 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121059 or CMSBurley 21 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121044, with asecond tobacco plant that comprises a desired trait to produce an F₁progeny seed; (b) growing the F₁ progeny seed and selecting an F₁progeny plant that comprises the desired trait; (c) crossing theselected F₁ progeny plant with a plant of the first tobacco cultivarBurley 21 SRC or CMS Burley 21 SRC to produce a backcross BC₁F₁ progenyseed; (d) growing the BC₁F₁ progeny seed and selecting a backcross BC₁F₁progeny plant comprising the desired trait and essentially all of thephysiological and morphological characteristics of the first tobaccocultivar Burley 21 SRC or CMS Burley 21 SRC; and (e) repeating steps (c)and (d) three or more times in succession to produce selected fourth orhigher backcross progeny that comprise the desired trait. In additionalaspects, steps (c) and (d) can be repeated one or more times insuccession to produce second or higher backcross progeny that comprisethe desired trait.

In another aspect, the present disclosure includes a method ofintroducing a desired trait into a tobacco cultivar comprising: (a)crossing a plant of a first tobacco cultivar Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121059 or CMS Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121044 with a plant of a secondtobacco cultivar that comprises a desired trait to produce a progenyplant where the desired trait is selected from the group consisting ofherbicide resistance, pest resistance, disease resistance, high yield,high grade index, curability, curing quality, mechanical harvestability,holding ability, leaf quality, height, plant maturation, early maturing,early to medium maturing, medium maturing, medium to late maturing, latematuring, small stalk, medium stalk, large stalk, leaf number per plant,5-10 leaves per plant, 11-15 leaves per plant, 16-21 leaves per plant,and any combination thereof, to produce an F₁ progeny seed; (b) growingthe F₁ progeny seed into an F₁ progeny plant and selecting the F₁progeny plant having the desired trait; (c) crossing the selected F₁progeny plant with a plant of the first tobacco cultivar Burley 21 SRC,a representative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121059 or CMS Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121044 to produce a backcrossprogeny plant seed; (d) growing the backcross progeny plant seed into abackcross progeny plant and selecting the backcross progeny plantcomprising the desired trait and essentially all of the physiologicaland morphological characteristics of the first tobacco cultivar, Burley21 SRC or CMS Burley 21 SRC; and (e) repeating steps (c) and (d) one ormore times in succession to produce a selected second, third, fourth orhigher backcross progeny plant that comprises the desired trait andessentially all of the physiological and morphological characteristicsof the first tobacco cultivar, Burley 21 SRC or CMS Burley 21 SRC.

In another aspect, the present disclosure includes a method ofintroducing a desired trait into a tobacco cultivar comprising: (a)crossing a plant of a first tobacco cultivar Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121059 or CMS Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121044, with a plant of a secondtobacco cultivar that comprises a desired trait to produce a progenyplant where the desired trait is selected from the group consisting ofherbicide resistance, pest resistance, disease resistance, high yield,high grade index, curability, curing quality, mechanical harvestability,holding ability, leaf quality, height, plant maturation, early maturing,early to medium maturing, medium maturing, medium to late maturing, latematuring, small stalk, medium stalk, large stalk, leaf number per plant,5-10 leaves per plant, 11-15 leaves per plant, 16-21 leaves per plant,and any combination thereof, to produce an F₁ progeny seed; (b) growingthe F₁ progeny seed into an F₁ progeny plant and selecting the F₁progeny plant having the desired trait; (c) crossing the selected F₁progeny plant with a plant of the first tobacco cultivar to produce abackcross progeny plant seed; (d) growing the backcross progeny plantseed into a backcross progeny plant and selecting the backcross progenyplant comprising the desired trait and essentially all of thephysiological and morphological characteristics of the first tobaccocultivar, Burley 21 SRC or CMS Burley 21 SRC; and (e) repeating steps(c) and (d) one or more times in succession to produce a selectedsecond, third, fourth or higher backcross progeny plant that comprisesthe desired trait and essentially all of the physiological andmorphological characteristics of the first tobacco cultivar, Burley 21SRC or CMS Burley 21 SRC.

In another aspect, the present disclosure includes a method ofintroducing a desired trait into a tobacco cultivar comprising: (a)crossing a plant of a first tobacco cultivar Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121059 or CMS Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121044, with a plant of a secondtobacco cultivar that comprises a desired trait to produce a progenyplant where the desired trait is selected from the group consisting ofherbicide resistance, pest resistance, disease resistance, high yield,high grade index, curability, curing quality, mechanical harvestability,holding ability, leaf quality, height, plant maturation, early maturing,early to medium maturing, medium maturing, medium to late maturing, latematuring, small stalk, medium stalk, large stalk, leaf number per plant,5-10 leaves per plant, 11-15 leaves per plant, 16-21 leaves per plant,and any combination thereof, to produce an F₁ progeny seed; (b) growingthe F₁ progeny seed into an F₁ progeny plant and selecting the F₁progeny plant having the desired trait; (c) crossing the selected F₁progeny plant with a plant of the first tobacco cultivar to produce abackcross progeny plant seed; (d) growing the backcross progeny plantseed into a backcross progeny plant and selecting the backcross progenyplant comprising the desired trait and essentially all of thephysiological and morphological characteristics of the first tobaccocultivar, Burley 21 SRC or CMS Burley 21 SRC; and (e) repeating steps(c) and (d) one or more times in succession to produce a selectedsecond, third, fourth or higher backcross progeny plant that comprisesthe desired trait and essentially all of the physiological andmorphological characteristics of the first tobacco cultivar, Burley 21SRC or CMS Burley 21 SRC, where the plant has a desired trait of diseaseresistance.

In an aspect, the present disclosure includes a method for producing atobacco plant having decreased nicotine conversion comprising:identifying a first tobacco plant comprising a nucleotide sequenceselected from the group consisting of the nucleotide sequence of SEQ IDNO: 1, SEQ ID NO: 2, SEQ ID NO: 11, and any combination thereof;crossing the first tobacco plant with a second tobacco plant andcollecting an F₁ seed; crossing a plant grown from the F₁ seed to athird tobacco plant and collecting a second tobacco seed; andidentifying a second tobacco seed or a plant grown from the second seedthat is homozygous for the nucleotide sequence selected from the groupconsisting of the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 11, and any combination thereof, where the first tobacco plant isa plant of tobacco cultivar Burley 21 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121059, or tobacco cultivar CMS Burley 21 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121044.

In an aspect, the present disclosure includes a method for producing atobacco plant having decreased nicotine conversion comprising:identifying a first tobacco plant comprising a nucleotide sequenceselected from the group consisting of the nucleotide sequence of SEQ IDNO: 1, SEQ ID NO: 2, SEQ ID NO: 11, and any combination thereof;crossing the first tobacco plant with a second tobacco plant andcollecting an F₁ seed; crossing a plant grown from the F₁ seed to athird tobacco plant and collecting a second tobacco seed; andidentifying a second tobacco seed or a plant grown from the second seedthat is homozygous for the nucleotide sequence selected from the groupconsisting of the nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQID NO: 11, and any combination thereof, where the third tobacco plant isa plant of tobacco cultivar Burley 21 SRC, a representative sample seedof the cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121059, or tobacco cultivar CMS Burley 21 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121044.

In another aspect, the present disclosure includes a method of producinga plant of a tobacco cultivar Burley 21 SRC, a representative sampleseed of the cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121059 or tobacco cultivar CMS Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121044 comprising at least one(e.g., 1, 2, 3, 4, 5, 6, 7, 8, and the like) additional desired traitcomprising the steps of: (a) collecting tissue capable of beingpropagated from a plant of a tobacco cultivar Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121059 or CMS Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121044; and (b) introducing a atleast one transgene (nucleic acid construct) conferring at least onedesired trait into the tissue.

In another aspect, the present disclosure includes a method of producingan herbicide resistant tobacco plant comprising transforming a tobaccoplant, or part thereof, produced by growing a seed of tobacco cultivarBurley 21 SRC, a representative sample seed of the cultivar having beendeposited with the ATCC under ATCC Accession No. PTA-121059 or tobaccocultivar CMS Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121044with at least one transgene (nucleic acid construct), where the at leastone transgene (nucleic acid construct) confers resistance to anherbicide selected from the group consisting of imidazolinone,cyclohexanedione, sulfonylurea, glyphosate, glufosinate, phenoxyproprionic acid, L-phosphinothricin, triazine, benzonitrile, and anycombination thereof.

In another aspect, the present disclosure includes an herbicideresistant tobacco plant produced by a method comprising transforming atobacco plant, or part thereof, produced by growing a seed of a tobaccocultivar Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121059or CMS Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121044with at least one transgene (nucleic acid construct), where the at leastone transgene (nucleic acid construct) confers resistance to anherbicide selected from the group consisting of imidazolinone,cyclohexanedione, sulfonylurea, glyphosate, glufosinate, phenoxyproprionic acid, L-phosphinothricin, triazine, benzonitrile, and anycombination thereof.

In another aspect, the present disclosure includes a method of producinga pest and/or insect resistant tobacco plant where the method comprisestransforming a tobacco plant produced by growing a seed of tobaccocultivar Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121059or tobacco cultivar CMS Burley 21 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121044, with at least one transgene (nucleic acid construct)that confers pest and/or insect resistance.

In a further aspect, the present disclosure includes a pest and/orinsect resistant tobacco plant produced by a method comprisingtransforming a tobacco plant produced by growing a seed of tobaccocultivar Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121059or tobacco cultivar CMS Burley 21 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121044, with at least one transgene (nucleic acid construct)that confers pest and/or insect resistance.

In a further aspect, the present disclosure includes a pest and/orinsect resistant tobacco plant produced by a method comprisingtransforming a tobacco plant produced by growing a seed of tobaccocultivar Burley 21 SRC, a representative sample seed of the cultivarhaving been deposited with the ATCC under ATCC Accession No. PTA-121059or tobacco cultivar CMS Burley 21 SRC, a representative sample seed ofthe cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121044, with at least one transgene (nucleic acid construct)that confers pest and/or insect resistance, where the transgene (nucleicacid construct) encodes a Bacillus thuringiensis (BT) endotoxin.

In another aspect, the present disclosure includes a method of producinga disease resistant tobacco plant, the method comprising transforming atobacco plant produced by growing a seed of tobacco cultivar Burley 21SRC, a representative sample seed of the cultivar having been depositedwith the ATCC under ATCC Accession No. PTA-121059 or tobacco cultivarCMS Burley 21 SRC, a representative sample seed of the cultivar havingbeen deposited with the ATCC under ATCC Accession No. PTA-121044 with atleast one transgene (nucleic acid construct) that confers diseaseresistance.

In a further aspect, the present disclosure includes a disease resistanttobacco plant produced by transforming a tobacco plant produced bygrowing a seed of tobacco cultivar Burley 21 SRC, a representativesample seed of the cultivar having been deposited with the ATCC underATCC Accession No. PTA-121059 or tobacco cultivar CMS Burley 21 SRC, arepresentative sample seed of the cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121044 with at least one transgene(nucleic acid construct) that confers disease resistance.

BRIEF DESCRIPTION OF THE SEQUENCES

SEQ ID NO: 1 sets forth a cyp82e4 W329Stop nucleotide sequence.

SEQ ID NO: 2 sets forth a cyp82e5v2 W422Stop nucleotide sequence.

SEQ ID NO: 3 sets forth a cyp82e4 W329Stop amino acid sequence.

SEQ ID NO: 4 sets forth a cyp82e5v2 W422Stop amino acid sequence.

SEQ ID NO: 5 sets forth a CYP82E4 wild-type nucleotide sequence.

SEQ ID NO: 6 sets forth a CYP82E5v2 wild-type nucleotide sequence.

SEQ ID NO: 7 sets forth a CYP82E4 wild-type amino acid sequence.

SEQ ID NO: 8 sets forth a CYP82E5v2 wild-type amino acid sequence.

SEQ ID NO: 9 sets forth a CYP82E10 wild-type nucleotide sequence.

SEQ ID NO: 10 sets forth a CYP82E10 wild-type amino acid sequence.

SEQ ID NO: 11 sets forth a CYP82E10 P381S nucleotide sequence.

SEQ ID NO: 12 sets forth a CYP82E10 P381S amino acid sequence.

All three tobacco Nicotine Demethylase genes (CYP82E4, CYP82E5v2,CYP82E10) share a common structure: a 939 bp exon 1 and a 612 bp exon 2separated by a large intron, whose length varies among the three genes.See Lewis et al., “Three nicotine demethylase genes mediate nornicotinebiosynthesis in Nicotiana tabacum L.: Functional characterization of theCYP82E10 gene,” Phytochemistry, 71 (2010), 1988-1998. SEQ ID NOs: 1, 2,5, 6, 9, and 11 set forth wild-type or mutant versions of codingsequences of CYP82E4, CYP82E5v2, and CYP82E10. It is understood that,used herein, a plant comprising, having, or homozygous for a sequenceselected from SEQ ID NOs: 1, 2, 5, 6, 9, and 11 refers to a plantcomprising at the CYP82E4, CYP82E5v2, or CYP82E10 endogenous locus agenomic sequence comprising the coding sequence of SEQ ID NO: 1, 2, 5,6, 9, or 11.

DETAILED DESCRIPTION OF THE DISCLOSURE

This description is not intended to be a detailed catalog of all thedifferent ways in which the disclosure may be implemented, or all thefeatures that may be added to the instant disclosure. For example,features illustrated with respect to one embodiment may be incorporatedinto other embodiments, and features illustrated with respect to aparticular embodiment may be deleted from that embodiment. Thus, thedisclosure contemplates that in some embodiments of the disclosure, anyfeature or combination of features set forth herein can be excluded oromitted. In addition, numerous variations and additions to the variousembodiments suggested herein will be apparent to those skilled in theart in light of the instant disclosure, which do not depart from theinstant disclosure. Hence, the following descriptions are intended toillustrate some particular embodiments of the disclosure, and not toexhaustively specify all permutations, combinations and variationsthereof.

Unless otherwise defined, all technical and scientific terms used hereinhave the same meaning as commonly understood by one of ordinary skill inthe art to which this disclosure belongs. The terminology used in thedescription of the disclosure herein is for the purpose of describingparticular embodiments only and is not intended to be limiting of thedisclosure.

All publications, patent applications, patents and other referencescited herein are incorporated by reference in their entireties for theteachings relevant to the sentence and/or paragraph in which thereference is presented. References to techniques employed herein areintended to refer to the techniques as commonly understood in the art,including variations on those techniques or substitutions of equivalenttechniques that would be apparent to one of skill in the art.

Unless the context indicates otherwise, it is specifically intended thatthe various features of the disclosure described herein can be used inany combination. Moreover, the present disclosure also contemplates thatin some embodiments of the disclosure, any feature or combination offeatures set forth herein can be excluded or omitted. To illustrate, ifthe specification states that a composition comprises components A, Band C, it is specifically intended that any of A, B or C, or acombination thereof, can be omitted and disclaimed singularly or in anycombination.

As used in the description of the disclosure and the appended claims,the singular forms “a,” “an” and “the” are intended to include theplural forms as well, unless the context clearly indicates otherwise.

As used herein, “and/or” refers to and encompasses any and all possiblecombinations of one or more of the associated listed items, as well asthe lack of combinations when interpreted in the alternative (“or”).

The term “about,” as used herein when referring to a measurable valuesuch as a dosage or time period and the like, is meant to encompassvariations of ±20%, ±10%, ±5%, ±1%, ±0.5%, or even ±0.1% of thespecified amount.

As used herein, phrases such as “between X and Y” and “between about Xand Y” should be interpreted to include X and Y. As used herein, phrasessuch as “between about X and Y” mean “between about X and about Y” andphrases such as “from about X to Y” mean “from about X to about Y.”

The terms “comprise,” “comprises” and “comprising” as used herein,specify the presence of the stated features, integers, steps,operations, elements, and/or components, but do not preclude thepresence or addition of one or more other features, integers, steps,operations, elements, components, and/or groups thereof.

As used herein, the transitional phrase “consisting essentially of”means that the scope of a claim is to be interpreted to encompass thespecified materials or steps recited in the claim and those that do notmaterially affect the basic and novel characteristic(s) of the claimeddisclosure. Thus, the term “consisting essentially of” when used in aclaim of this disclosure is not intended to be interpreted to beequivalent to “comprising.”

“Introducing,” in the context of a polynucleotide sequence (e.g., arecombinant polynucleotide and/or expression cassette of thedisclosure), means presenting a polynucleotide sequence to the plant,plant part, and/or plant cell in such a manner that the polynucleotidesequence gains access to the interior of a cell. Where more than onepolynucleotide sequence is to be introduced these polynucleotidesequences can be assembled as part of a single polynucleotide or nucleicacid construct, or as separate polynucleotide or nucleic acidconstructs, and can be located on the same or different transformationvectors. Accordingly, these polynucleotides can be introduced into plantcells in a single transformation event, in separate transformationevents, or, e.g., as part of a breeding protocol. Thus, the term“transformation” as used herein refers to the introduction of aheterologous nucleic acid into a cell. Transformation of a cell may bestable or transient. Thus, in some embodiments, a plant cell, plant partor plant of this disclosure can be stably transformed with a recombinantpolynucleotide of the disclosure. In other embodiments, a plant cell,plant part or plant of this disclosure can be transiently transformedwith a recombinant polynucleotide of the disclosure.

“Tobacco product” is defined as “any product made or derived fromtobacco that is intended for human use or consumption, including anycomponent, part, or accessory of a tobacco product (except for rawmaterials other than tobacco used in manufacturing a component, part, oraccessory of a tobacco product)” (section 201 of the FD&C Act; 21 U.S.C.321). The label and packaging is part of a tobacco product.

Terms “nicotine conversion rate,” “percent nicotine conversion,” and“percentage nicotine conversion” are used interchangeably. Percentnicotine demethylation in a sample is calculated by dividing the levelof nornicotine by the combined level of nicotine and nornicotine asmeasured in the sample, and multiplying by 100.

Banket A1 SRC

In some aspects, the present disclosure provides tobacco cultivars, andparts thereof, from Banket A1 SRC, representative sample seeds of thiscultivar having been deposited with the ATCC under ATCC Accession No.PTA-121043. In other aspects, the present disclosure provides a tobaccoplant, or part thereof, produced by growing a seed of Banket A1 SRC. Infurther aspects, a plant of the present disclosure can include a plantwith all, or essentially all, of the morphological and physiologicalcharacteristics of cultivar Banket A1 SRC.

While not being limited by process, Banket A1 SRC is a result of theintroduction of three mutated CYP82E genes in a burley tobacco cultivarBanket A1. The three genes are a mutated CYP82E4 gene recited as 325-6#775 in Lewis et al. (“Three nicotine demethylase genes mediatenornicotine biosynthesis in Nicotiana tabacum L.: Functionalcharacterization of the CYP82E10 gene,” Phytochemistry, 71 (2010),1988-1998 (SEQ ID NO: 1, which sets forth a cyp82e4 W329Stop, herebyincorporated by reference in its entirety)), a mutated CYP82E5v2 recitedin Lewis et al. (supra) as 325-6 #1-13 (SEQ ID NO: 2, which sets forth acyp82e5v2 W422Stop, hereby incorporated by reference in its entirety),and a mutated CYP82E10 recited in Lewis et al. (supra) as 325-6 #1041(SEQ ID NO:11, which sets forth a cyp82e10 P381S, hereby incorporated byreference in its entirety). Mutations cyp82e4 W329Stop and cyp82e5v2W422Stop result in truncated proteins while cyp82E10 P381S results in anonfunctional protein. A cyp82e4 W329Stop (“e4”), a cyp82e5v2 W422Stop(“e5”), and a cyp82e10 P381S (“e10”) mutation are introduced from ae4e4|e5e5|e10e10 triple mutant from a strong converter burleybackground, line DH98-325-6, as listed in Table 4 of Lewis et al.(supra) into a burley tobacco cultivar Banket A1 background.

Banket A1 SRC is the result of seven backcrosses with burley cultivarBanket A1 as the recurrent parent, followed by two rounds of selfingwith selection for homozygosity for the cyp82e4 W329Stop, the cyp82e5v2W422Stop, and cyp82e10 P381S mutations to yield BC₇F₃ plants (Banket A1SRC) in which the wild-type CYP82E4, CYP82E5v2 and CYP82E10 alleles ofBanket A1 are replaced by the mutant alleles (e.g., cyp82e4 W329Stop,cyp82e5v2 W422Stop, and cyp82e10 P381S).

Banket A1 SRC progeny plants have genetic backgrounds that are at least95%, at least 97%, at least 98%, or at least 99% similar to Banket A1.Banket A1 SRC plants exhibit low nornicotine levels and produce leaveswith reduced potential for accumulating derived NNN during curing,storage, and smoking.

CMS Banket A1 SRC

In some aspects, the present disclosure provides tobacco cultivars, andparts thereof, from CMS Banket A1 SRC, representative sample seeds ofthis cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121054. In further aspects, the present disclosure also includesa tobacco plant, or part thereof, produced by growing a seed of CMSBanket A1 SRC. In still further aspects, a plant of the presentdisclosure can include a plant with all, or essentially all, of themorphological and physiological characteristics of cultivar CMS BanketA1 SRC. CMS Banket A1 SRC is a male-sterile (CMS) version of Banket A1SRC (CMS Banket A1 SRC) produced by crossing a plant of CMS Banket A1 asa female with pollen of Banket A1 SRC BC₆F₁ to produce male-sterileplants heterozygous for all three mutations. The CMS progeny plants ofthe CMS Banket A1×Banket A1 SRC BC₆F₁ cross are male sterile. Aplurality of CMS Banket A1×Banket A1 SRC BC₆F₁ x CMS plants (e.g., CMSF₁ progeny plants) are screened for the cyp82e4 W329Stop, cyp82e5v2W422Stop, and cyp82e10 P381S mutations to identify plants heterozygousfor all three mutations. A single male-sterile plant resulting from thiscross is backcrossed as a female to Banket A1 SRC to prepare BC₇F₁ CMSprogeny. BC₇F₁ CMS progeny homozygous for the cyp82e4 W329Stop, thecyp82e5v2 W422Stop, and cyp82e10 P381S mutations are identified bygenotyping and designated as CMS Banket A1 SRC. Because the CMS BanketA1 SRC line is male sterile, it is maintained via pollination withBanket A1 SRC. Banket A1 SRC is crossed as the male parent to CMS BanketA1 SRC to prepare CMS Banket A1 SRC F₁ progeny plants.

CMS Banket A1 SRC and CMS Banket A1 SRC F₁ progeny plants have geneticbackgrounds that are at least 95%, at least 97%, at least 98%, or atleast 99% similar to Banket A1. CMS Banket A1 SRC and CMS Banket A1 SRCF₁ progeny plants exhibit low nornicotine levels and produce leaves withreduced potential for accumulating derived NNN during curing, storage,and smoking.

NC 2000 SRC

In some aspects, the present disclosure provides tobacco cultivars, andparts thereof, from NC 2000 SRC, representative sample seeds of thiscultivar having been deposited with the ATCC under ATCC Accession No.PTA-121053. In other aspects, the present disclosure provides a tobaccoplant, or part thereof, produced by growing a seed of NC 2000 SRC. Infurther aspects, a plant of the present disclosure can include a plantwith all, or essentially all, of the morphological and physiologicalcharacteristics of cultivar NC 2000 SRC.

While not being limited by process, NC 2000 SRC is a result of theintroduction of three mutated CYP82E genes in a burley tobacco cultivarNC 2000. The three genes are a mutated CYP82E4 gene recited as 325-6#775 in Lewis et al. (“Three nicotine demethylase genes mediatenornicotine biosynthesis in Nicotiana tabacum L.: Functionalcharacterization of the CYP82E10 gene,” Phytochemistry, 71 (2010),1988-1998 (SEQ ID NO: 1, which sets forth a cyp82e4 W329Stop, herebyincorporated by reference in its entirety)), a mutated CYP82E5v2 recitedin Lewis et al. (supra) as 325-6 #1-13 (SEQ ID NO: 2, which sets forth acyp82e5v2 W422Stop, hereby incorporated by reference in its entirety),and a mutated CYP82E10 recited in Lewis et al. (supra) as 325-6 #1041(SEQ ID NO:11, which sets forth a cyp82e10 P381S, hereby incorporated byreference in its entirety). Mutations cyp82e4 W329Stop and cyp82e5v2W422Stop result in truncated proteins while cyp82E10 P381S results in anonfunctional protein. A cyp82e4 W329Stop (“e4”), a cyp82e5v2 W422Stop(“e5”), and a cyp82e10 P381S (“e10”) mutation are introduced from ae4e4|e5e5|e10e10 triple mutant from a strong converter burleybackground, line DH98-325-6, as listed in Table 4 of Lewis et al.(supra) into a burley tobacco cultivar NC 2000 background.

NC 2000 SRC is the result of seven backcrosses with burley cultivar NC2000 as the recurrent parent, followed by two rounds of selling withselection for homozygosity for the cyp82e4 W329Stop, the cyp82e5v2W422Stop, and cyp82e10 P381S mutations to yield BC₇F₃ plants (NC 2000SRC) in which the wild-type CYP82E4, CYP82E5v2 and CYP82E10 alleles ofNC 2000 are replaced by the mutant alleles (e.g., cyp82e4 W329Stop,cyp82e5v2 W422Stop, and cyp82e10 P381S).

NC 2000 SRC progeny plants have genetic backgrounds that are at least95%, at least 97%, at least 98%, or at least 99% similar to NC 2000. NC2000 SRC plants exhibit low nornicotine levels and produce leaves withreduced potential for accumulating derived NNN during curing, storage,and smoking.

CMS NC 2000 SRC

In some aspects, the present disclosure provides tobacco cultivars, andparts thereof, from CMS NC 2000 SRC, representative sample seeds of thiscultivar having been deposited with the ATCC under ATCC Accession No.PTA-121045. In further aspects, the present disclosure also includes atobacco plant, or part thereof, produced by growing a seed of CMS NC2000 SRC. In still further aspects, a plant of the present disclosurecan include a plant with all, or essentially all, of the morphologicaland physiological characteristics of cultivar CMS NC 2000 SRC. CMS NC2000 SRC is a male-sterile (CMS) version of NC 2000 SRC (CMS NC 2000SRC) produced by crossing a plant of CMS NC 2000 as a female with pollenof NC 2000 SRC BC₆F₁ to produce male-sterile plants heterozygous for allthree mutations. The CMS progeny plants of the CMS NC 2000×NC 2000 SRCBC₆F₁ cross are male sterile. A plurality of CMS NC 2000×NC 2000 SRCBC₆F₁×plants (e.g., CMS F₁ progeny plants) are screened for the cyp82e4W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S mutations to identifyplants heterozygous for all three mutations. A single male-sterile plantresulting from this cross is backcrossed as a female to NC 2000 SRC toprepare BC₇F₁ CMS progeny. BC₇F₁ CMS progeny homozygous for the cyp82e4W329Stop, the cyp82e5v2 W422Stop, and cyp82e10 P381S mutations areidentified by genotyping and designated as CMS NC 2000 SRC. Because theCMS NC 2000 SRC line is male sterile, it is maintained via pollinationwith NC 2000 SRC. NC 2000 SRC is crossed as the male parent to CMS NC2000 SRC to prepare CMS NC 2000 SRC F₁ progeny plants. CMS NC 2000 SRCand CMS NC 2000 SRC F₁ progeny plants have genetic backgrounds that areat least 95%, at least 97%, at least 98%, or at least 99% similar to NC2000. CMS NC 2000 SRC and CMS NC 2000 SRC F₁ progeny plants exhibit lownornicotine levels and produce leaves with reduced potential foraccumulating derived NNN during curing, storage, and smoking.

NC 2002 SRC

In some aspects, the present disclosure provides tobacco cultivars, andparts thereof, from NC 2002 SRC, representative sample seeds of thiscultivar having been deposited with the ATCC under ATCC Accession No.PTA-121058. In other aspects, the present disclosure provides a tobaccoplant, or part thereof, produced by growing a seed of NC 2002 SRC. Infurther aspects, a plant of the present disclosure can include a plantwith all, or essentially all, of the morphological and physiologicalcharacteristics of cultivar NC 2002 SRC.

While not being limited by process, NC 2002 SRC is a result of theintroduction of three mutated CYP82E genes in a burley tobacco cultivarNC 2002LC. The three genes are a mutated CYP82E4 gene recited as 325-6#775 in Lewis et al. (“Three nicotine demethylase genes mediatenornicotine biosynthesis in Nicotiana tabacum L.: Functionalcharacterization of the CYP82E10 gene,” Phytochemistry, 71 (2010),1988-1998 (SEQ ID NO: 1, which sets forth a cyp82e4 W329Stop, herebyincorporated by reference in its entirety)), a mutated CYP82E5v2 recitedin Lewis et al. (supra) as 325-6 #1-13 (SEQ ID NO: 2, which sets forth acyp82e5v2 W422Stop, hereby incorporated by reference in its entirety),and a mutated CYP82E10 recited in Lewis et al. (supra) as 325-6 #1041(SEQ ID NO:11, which sets forth a cyp82e10 P381S, hereby incorporated byreference in its entirety). Mutations cyp82e4 W329Stop and cyp82e5v2W422Stop result in truncated proteins while cyp82E10 P381S results in anonfunctional protein. A cyp82e4 W329Stop (“e4”), a cyp82e5v2 W422Stop(“e5”), and a cyp82e10 P381 S (“e10”) mutation are introduced from ae4e4|e5e5|e10e10 triple mutant from a strong converter burleybackground, line DH98-325-6, as listed in Table 4 of Lewis et al.(supra) into a burley tobacco cultivar NC 2002LC background.

NC 2002 SRC is the result of seven backcrosses with burley cultivar NC2002LC as the recurrent parent, followed by two rounds of selfing withselection for homozygosity for the cyp82e4 W329Stop, the cyp82e5v2W422Stop, and cyp82e10 P381S mutations to yield BC₇F₃ plants (NC 2002SRC) in which the wild-type CYP82E4, CYP82E5v2 and CYP82E10 alleles ofNC 2002LC are replaced by the mutant alleles (e.g., cyp82e4 W329Stop,cyp82e5v2 W422Stop, and cyp82e10 P381S).

NC 2002 SRC progeny plants have genetic backgrounds that are at least95%, at least 97%, at least 98%, or at least 99% similar to NC 2002LC.NC 2002 SRC plants exhibit low nornicotine levels and produce leaveswith reduced potential for accumulating derived NNN during curing,storage, and smoking.

CMS NC 2002 SRC

In some aspects, the present disclosure provides tobacco cultivars, andparts thereof, from CMS NC 2002 SRC, representative sample seeds of thiscultivar having been deposited with the ATCC under ATCC Accession No.PTA-121041. In further aspects, the present disclosure also includes atobacco plant, or part thereof, produced by growing a seed of CMS NC2002 SRC. In still further aspects, a plant of the present disclosurecan include a plant with all, or essentially all, of the morphologicaland physiological characteristics of cultivar CMS NC 2002 SRC. CMS NC2002 SRC is a male-sterile (CMS) version of NC 2002 SRC (CMS NC 2002SRC) produced by crossing a plant of CMS NC 2002LC as a female withpollen of NC 2002 SRC BC₆F₁ to produce male-sterile plants heterozygousfor all three mutations. The CMS progeny plants of the CMS NC 2002LC×NC2002 SRC BC₆F₁ cross are male sterile. A plurality of CMS NC 2002LC×NC2002 SRC BC₆F₁×plants (e.g., CMS F₁ progeny plants) are screened for thecyp82e4 W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381 S mutations toidentify plants heterozygous for all three mutations. A singlemale-sterile plant resulting from this cross is backcrossed as a femaleto NC 2002 SRC to prepare BC₇F₁ CMS progeny. BC₇F₁ CMS progenyhomozygous for the cyp82e4 W329Stop, the cyp82e5v2 W422Stop, andcyp82e10 P381S mutations are identified by genotyping and designated asCMS NC 2002 SRC. Because the CMS NC 2002 SRC line is male sterile, it ismaintained via pollination with NC 2002 SRC. NC 2002 SRC is crossed asthe male parent to CMS NC 2002 SRC to prepare CMS NC 2002 SRC F₁ progenyplants.

CMS NC 2002 SRC and CMS NC 2002 SRC F₁ progeny plants have geneticbackgrounds that are at least 95%, at least 97%, at least 98%, or atleast 99% similar to NC 2002LC. CMS NC 2002 SRC and CMS NC 2002 SRC F₁progeny plants exhibit low nornicotine levels and produce leaves withreduced potential for accumulating derived NNN during curing, storage,and smoking.

Burley 21 SRC

In some aspects, the present disclosure provides tobacco cultivars, andparts thereof, from Burley 21 SRC, representative sample seeds of thiscultivar having been deposited with the ATCC under ATCC Accession No.PTA-121059. In other aspects, the present disclosure provides a tobaccoplant, or part thereof, produced by growing a seed of Burley 21 SRC. Infurther aspects, a plant of the present disclosure can include a plantwith all, or essentially all, of the morphological and physiologicalcharacteristics of cultivar Burley 21 SRC.

While not being limited by process, Burley 21 SRC is a result of theintroduction of three mutated CYP82E genes in a burley tobacco cultivarBurley 21 SRC. The three genes are a mutated CYP82E4 gene recited as325-6 #775 in Lewis et al. (“Three nicotine demethylase genes mediatenornicotine biosynthesis in Nicotiana tabacum L.: Functionalcharacterization of the CYP82E10 gene,” Phytochemistry, 71 (2010),1988-1998 (SEQ ID NO: 1, which sets forth a cyp82e4 W329Stop, herebyincorporated by reference in its entirety)), a mutated CYP82E5v2 recitedin Lewis et al. (supra) as 325-6 #1-13 (SEQ ID NO: 2, which sets forth acyp82e5v2 W422Stop, hereby incorporated by reference in its entirety),and a mutated CYP82E10 recited in Lewis et al. (supra) as 325-6 #1041(SEQ ID NO:11, which sets forth a cyp82e10 P381 S, hereby incorporatedby reference in its entirety). Mutations cyp82e4 W329Stop and cyp82e5v2W422Stop result in truncated proteins while cyp82E10 P381S results in anonfunctional protein. A cyp82e4 W329Stop (“e4”), a cyp82e5v2 W422Stop(“e5”), and a cyp82e10 P381S (“e10”) mutation are introduced from ae4e4|e5e5|e10e10 triple mutant from a strong converter burleybackground, line DH98-325-6, as listed in Table 4 of Lewis et al.(supra) into a burley tobacco cultivar Burley 21 SRC background.

Burley 21 SRC is the result of seven backcrosses with burley cultivarBurley 21 SRC as the recurrent parent, followed by two rounds of selfingwith selection for homozygosity for the cyp82e4 W329Stop, the cyp82e5v2W422Stop, and cyp82e10 P381S mutations to yield BC₇F₃ plants (Burley 21SRC) in which the wild-type CYP82E4, CYP82E5v2 and CYP82E10 alleles ofBurley 21 SRC are replaced by the mutant alleles (e.g., cyp82e4W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S).

Burley 21 SRC progeny plants have genetic backgrounds that are at least95%, at least 97%, at least 98%, or at least 99% similar to Burley 21SRC. Burley 21 SRC plants exhibit low nornicotine levels and produceleaves with reduced potential for accumulating derived NNN duringcuring, storage, and smoking.

CMS Burley 21 SRC

In some aspects, the present disclosure provides tobacco cultivars, andparts thereof, from CMS Burley 21 SRC, representative sample seeds ofthis cultivar having been deposited with the ATCC under ATCC AccessionNo. PTA-121044. In further aspects, the present disclosure also includesa tobacco plant, or part thereof, produced by growing a seed of CMSBurley 21 SRC. In still further aspects, a plant of the presentdisclosure can include a plant with all, or essentially all, of themorphological and physiological characteristics of cultivar CMS Burley21 SRC. CMS Burley 21 SRC is a male-sterile (CMS) version of Burley 21SRC (CMS Burley 21 SRC) produced by crossing a plant of CMS Burley 21 asa female with pollen of Burley 21 SRC BC₆F₁ to produce male-sterileplants heterozygous for all three mutations. The CMS progeny plants ofthe CMS Burley 21×Burley 21 SRC BC₆F₁ cross are male sterile. Aplurality of CMS Burley 21×Burley 21 SRC BC₆F₁×plants (e.g., CMS F₁progeny plants) are screened for the cyp82e4 W329Stop, cyp82e5v2W422Stop, and cyp82e10 P381S mutations to identify plants heterozygousfor all three mutations. A single male-sterile plant resulting from thiscross is backcrossed as a female to Burley 21 SRC to prepare BC₇F₁ CMSprogeny. BC₇F₁ CMS progeny homozygous for the cyp82e4 W329Stop, thecyp82e5v2 W422Stop, and cyp82e10 P381 S mutations are identified bygenotyping and designated as CMS Burley 21 SRC. Because the CMS Burley21 SRC line is male sterile, it is maintained via pollination withBurley 21 SRC. Burley 21 SRC is crossed as the male parent to CMS Burley21 SRC to prepare CMS Burley 21 SRC F₁ progeny plants.

CMS Burley 21 SRC and CMS Burley 21 SRC F₁ progeny plants have geneticbackgrounds that are at least 95%, at least 97%, at least 98%, or atleast 99% similar to Burley 21 SRC. CMS Burley 21 SRC and CMS Burley 21SRC F₁ progeny plants exhibit low nornicotine levels and produce leaveswith reduced potential for accumulating derived NNN during curing,storage, and smoking.

Other Plants

The present disclosure includes a tobacco seed produced by crossing twoparent tobacco plants and harvesting the resultant tobacco seed, whereat least one parent tobacco plant is Banket A1 SRC. In one aspect, theBanket A1 SRC is the male parent plant. In another aspect, the CMSBanket A1 SRC is the female parent plant. One aspect of the presentdisclosure provides tobacco plants that are homozygous at the cyp82e4,cyp82e5v2, and cyp82E10 loci for SEQ ID NO: 1, SEQ ID NO: 2, and SEQ IDNO: 11, respectively, and which share a genetic background that isgreater than about 75%, 80%, 85%, 90%, 95%, 98%, or 99% similar toBanket A1 or CMS Banket A1. In one aspect, approximately or greater thanabout 50%, 75%, or 100% of a progeny's genetics is provided by a plantof the present disclosure that is homozygous at the cyp82e4, cyp82e5v2,and cyp82E10 loci for SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 11,respectively. In one aspect, a plant of the present disclosure has agenetic background that is at least 95%, at least 97%, at least 98%, orat least 99% similar to Banket A1 or CMS Banket A1. In another aspect, aplant of the present disclosure exhibits low nornicotine and is notsubject to conversion to high nornicotine. In one aspect, a plant of thepresent disclosure is the progeny plant of a female or male parent plantthat is Fusarium wilt resistant. In another aspect, a plant of Banket A1SRC has low resistance to black shank and moderate resistance tobacterial wilt.

The present disclosure includes a tobacco seed produced by crossing twoparent tobacco plants and harvesting the resultant tobacco seed, whereat least one parent tobacco plant is NC 2000 SRC. In one aspect, the NC2000 SRC is the male parent plant. In another aspect, the CMS NC 2000SRC is the female parent plant. One aspect of the present disclosureprovides tobacco plants that are homozygous at the cyp82e4, cyp82e5v2,and cyp82E10 loci for SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 11,respectively, and which share a genetic background that is greater thanabout 75%, 80%, 85%, 90%, 95%, 98%, or 99% similar to NC 2000 or CMS NC2000. In one aspect, approximately or greater than about 50%, 75%, or100% of a progeny's genetics is provided by a plant of the presentdisclosure that is homozygous at the cyp82e4, cyp82e5v2, and cyp82E10loci for SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 11, respectively. Inone aspect, a plant of the present disclosure has a genetic backgroundthat is at least 95%, at least 97%, at least 98%, or at least 99%similar to NC 2000 or CMS NC 2000. In another aspect, a plant of thepresent disclosure exhibits low nornicotine and is not subject toconversion to high nornicotine. In one aspect, a plant of the presentdisclosure is the progeny plant of a female or male parent plant that isFusarium wilt resistant. In another aspect, a plant of NC 2000 SRC haslow resistance to black shank and moderate resistance to bacterial wilt.

The present disclosure includes a tobacco seed produced by crossing twoparent tobacco plants and harvesting the resultant tobacco seed, whereat least one parent tobacco plant is NC 2002 SRC. In one aspect, the NC2002 SRC is the male parent plant. In another aspect, the CMS NC 2002SRC is the female parent plant. One aspect of the present disclosureprovides tobacco plants that are homozygous at the cyp82e4, cyp82e5v2,and cyp82E10 loci for SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 11,respectively, and which share a genetic background that is greater thanabout 75%, 80%, 85%, 90%, 95%, 98%, or 99% similar to NC 2002LC or CMSNC 2002LC. In one aspect, approximately or greater than about 50%, 75%,or 100% of a progeny's genetics is provided by a plant of the presentdisclosure that is homozygous at the cyp82e4, cyp82e5v2, and cyp82E10loci for SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 11, respectively. Inone aspect, a plant of the present disclosure has a genetic backgroundthat is at least 95%, at least 97%, at least 98%, or at least 99%similar to NC 2002LC or CMS NC 2002LC. In another aspect, a plant of thepresent disclosure exhibits low nornicotine and is not subject toconversion to high nornicotine. In one aspect, a plant of the presentdisclosure is the progeny plant of a female or male parent plant that isFusarium wilt resistant. In another aspect, a plant of NC 2002 SRC haslow resistance to black shank and moderate resistance to bacterial wilt.

The present disclosure includes a tobacco seed produced by crossing twoparent tobacco plants and harvesting the resultant tobacco seed, whereat least one parent tobacco plant is Burley 21 SRC. In one aspect, theBurley 21 SRC is the male parent plant. In another aspect, the CMSBurley 21 SRC is the female parent plant. One aspect of the presentdisclosure provides tobacco plants that are homozygous at the cyp82e4,cyp82e5v2, and cyp82E10 loci for SEQ ID NO: 1, SEQ ID NO: 2, and SEQ IDNO: 11, respectively, and which share a genetic background that isgreater than about 75%, 80%, 85%, 90%, 95%, 98%, or 99% similar toBurley 21 SRC or CMS Burley 21. In one aspect, approximately or greaterthan about 50%, 75%, or 100% of a progeny's genetics is provided by aplant of the present disclosure that is homozygous at the cyp82e4,cyp82e5v2, and cyp82E10 loci for SEQ ID NO: 1, SEQ ID NO: 2, and SEQ IDNO: 11, respectively. In one aspect, a plant of the present disclosurehas a genetic background that is at least 95%, at least 97%, at least98%, or at least 99% similar to Burley 21 SRC or CMS Burley 21. Inanother aspect, a plant of the present disclosure exhibits lownornicotine and is not subject to conversion to high nornicotine. In oneaspect, a plant of the present disclosure is the progeny plant of afemale or male parent plant that is Fusarium wilt resistant. In anotheraspect, a plant of Burley 21 SRC has low resistance to black shank andmoderate resistance to bacterial wilt.

In one aspect, a plant of the present disclosure is a medium-latematuring variety with moderately high yield potential. In anotheraspect, a plant of the present disclosure offers a broad range ofimportant agronomic characteristics. In a further aspect, a plant of thepresent disclosure has one, two, three, four or more of the traitsincluding moderate resistance to black shank, some tolerance to bluemold, black root rot resistance, and resistance to common virusdiseases. In another aspect, a plant of the present disclosure has bluemold tolerance and level 4 resistance to both races of black shank andhigh root rot resistance. In one aspect, a plant of the presentdisclosure, such as Banket A1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMSNC 2000 SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley 21 SRC, or CMS Burley21 SRC, lacks Fusarium wilt resistance. In another aspect, a plant ofthe present disclosure is Fusarium wilt resistant. In another aspect, aplant of the present disclosure has low resistance to black shank andmoderate resistance to bacterial wilt.

In an aspect, the plants of the present disclosure have reduced oreliminated ability to convert nicotine to nornicotine. In an aspect, thepercentage nicotine conversion can be less than about 75%, 70%, 60%,50%, or 25% of that found in Banket A1. In other aspects, the nicotineconversion in plants of the present disclosure, including, e.g., BanketA1 SRC or CMS Banket A1 SRC, can be less than about 4%, about 3.5%,about 3%, about 2.5%, about 2%, about 1.5%, about 1%, or any rangetherein. In still other aspects, the nicotine conversion in plants ofthe present disclosure, including, e.g., Banket A1 SRC or CMS Banket A1SRC, can be in a range from about 3% to about 1%, about 3% to about0.5%, or about 2% to about 0.5%. In a preferred aspect, the percentagenicotine conversion is less than about 25%, 10%, 5%, or 2% of that foundin Banket A1 without the cyp82e4 W329Stop, the cyp82e5v2 W422Stop, andcyp82e10 P381 S mutations.

In an aspect, the plants of the present disclosure have reduced oreliminated ability to convert nicotine to nornicotine. In an aspect, thepercentage nicotine conversion can be less than about 75%, 70%, 60%,50%, or 25% of that found in NC 2000. In other aspects, the nicotineconversion in plants of the present disclosure, including, e.g., NC 2000SRC or CMS NC 2000 SRC, can be less than about 4%, about 3.5%, about 3%,about 2.5%, about 2%, about 1.5%, about 1%, or any range therein. Instill other aspects, the nicotine conversion in plants of the presentdisclosure, including, e.g., NC 2000 SRC or CMS NC 2000 SRC, can be in arange from about 3% to about 1%, about 3% to about 0.5%, or about 2% toabout 0.5%. In a preferred aspect, the percentage nicotine conversion isless than about 25%, 10%, 5%, or 2% of that found in NC 2000 without thecyp82e4 W329Stop, the cyp82e5v2 W422Stop, and cyp82e10 P381S mutations.

In an aspect, the plants of the present disclosure have reduced oreliminated ability to convert nicotine to nornicotine. In an aspect, thepercentage nicotine conversion can be less than about 75%, 70%, 60%,50%, or 25% of that found in NC 2002. In other aspects, the nicotineconversion in plants of the present disclosure, including, e.g., NC 2002SRC or CMS NC 2002 SRC, can be less than about 4%, about 3.5%, about 3%,about 2.5%, about 2%, about 1.5%, about 1%, or any range therein. Instill other aspects, the nicotine conversion in plants of the presentdisclosure, including, e.g., NC 2002 SRC or CMS NC 2002 SRC, can be in arange from about 3% to about 1%, about 3% to about 0.5%, or about 2% toabout 0.5%. In a preferred aspect, the percentage nicotine conversion isless than about 25%, 10%, 5%, or 2% of that found in NC 2002LC withoutthe cyp82e4 W329Stop, the cyp82e5v2 W422Stop, and cyp82e10 P381Smutations. In an aspect, the tobacco plants of the present disclosurecan have a nicotine conversion rate of about 3.5, 3.25, 3.0 or 2.75% orless.

In an aspect, the plants of the present disclosure have reduced oreliminated ability to convert nicotine to nornicotine. In an aspect, thepercentage nicotine conversion can be less than about 75%, 70%, 60%,50%, or 25% of that found in Burley 21 SRC. In other aspects, thenicotine conversion in plants of the present disclosure, including,e.g., Burley 21 SRC or CMS Burley 21 SRC, can be less than about 4%,about 3.5%, about 3%, about 2.5%, about 2%, about 1.5%, about 1%, or anyrange therein. In still other aspects, the nicotine conversion in plantsof the present disclosure, including, e.g., Burley 21 SRC or CMS Burley21 SRC, can be in a range from about 3% to about 1%, about 3% to about0.5%, or about 2% to about 0.5%. In a preferred aspect, the percentagenicotine conversion is less than about 25%, 10%, 5%, or 2% of that foundin Burley 21 SRC without the cyp82e4 W329Stop, the cyp82e5v2 W422Stop,and cyp82e10 P381S mutations.

In an aspect, the tobacco plants of the present disclosure can have anicotine conversion rate of about 3.5, 3.25, 3.0 or 2.75% or less. Inanother aspect, the nicotine conversion rate of tobacco plants of thepresent disclosure can be about 4.0, 3.9, 3.8, 3.7, 3.6, 3.5, 3.4, 3.3,3.2, 3.1, 3.0, 2.9, 2.8, 2.7, 2.6, 2.5, 2.4, 2.3, 2.2, 2.1, 2.0, 1.9,1.8, 1.7, 1.6, 1.5, 1.4, 1.3, 1.2, 1.1, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5% orless or any range therein. In another aspect, the nicotine conversionrate of tobacco plants of the present disclosure can be about 2.9, 2.8,2.7, 2.6, 2.5, 2.4, 2.3, 2.2, 2.1, 2.0, 1.9, 1.8, 1.7, 1.6, 1.5, 1.4,1.3, 1.2, 1.1, 1.0, 0.9, 0.8, 0.7, 0.6% or less or any range therein. Inanother aspect, the nicotine conversion rates can be in a range fromabout 0.5% to about 0.9%, about 0.5% to about 1.5%, about 0.5% to about2.0%, about 0.5% to about 2.5%, about 0.5% to about 2.75%, and about0.5% to about 3.0%. In another aspect, the nicotine conversion rates canbe in a range from about 1.0% to about 1.5%, about 1.0% to about 1.75%,about 1.0% to about 2.0%, about 1.0% to about 2.5%, about 1.0% to about2.75%, or about 1.0% to about 3.0%. In another aspect, the nicotineconversion rate in a plant of the present disclosure can be less thanabout 2.9, 2.75, 2.5, 2.25, 2.0, 1.9, 1.8, 1.7, 1.6, 1.5, 1.4, 1.3, 1.2,1.1 or 1.0% or any range therein.

In another aspect, the tobacco plants of the present disclosuretypically have a reduced amount of nornicotine of less than about 0.10%dry weight. For example, the nornicotine content in such plants can beabout 1.2, 1.0, 0.7, 0.5, 0.4, 0.2, 0.1, 0.09, 0.085, 0.08, 0.075, 0.07,0.065, 0.06, 0.055, 0.05, 0.045, 0.04, 0.035, 0.025, 0.01, 0.009,0.0075, 0.005, 0.0025, 0.001, 0.0009, 0.00075, 0.0005, 0.00025, or0.0001% dry weight, or undetectable, or any range therein. In anotheraspect, the nornicotine content can be less than about 1.2, 1.0, 0.9,0.8, 0.7, 0.5, 0.4, 0.2, 0.1, 0.075, 0.05, 0.025, 0.01, 0.009, 0.0075,0.005, 0.0025, 0.001, 0.0009, 0.00075, 0.0005, 0.00025, or 0.0001% dryweight, or any range therein. In another aspect, the nornicotine contentin such plants can be in a range from about 1.2% to about 1.0%, about0.7% to about 0.5%, about 0.4% to about 0.2%, about 0.1% to about0.075%, about 0.05% to about 0.025%, about 0.01% to about 0.0075%, about0.005% to about 0.0025%, about 0.001% to about 0.00075%, about 0.0005%to about 0.00025%, or about 0.0005% to about 0.0001% dry weight. In someaspects, in a plant of the present disclosure, the nornicotine is arelatively small percentage of total alkaloids in the plant compared toa commercial seedlot of Banket A1, NC 2000, NC 2002, or Burley 21. Insome aspects, the nornicotine in a plant of the present disclosure canbe about 2% to about 1%, less than 3%, about 2%, about 1.5%, about 1%,or 0.75% of total alkaloids. Tobacco products having a reduced amount ofnitrosamine content can be manufactured using tobacco plant materialfrom plants and plant parts of the present disclosure. Thus, in someembodiments, a tobacco product manufactured using tobacco plant materialfrom plants and plant parts of the present disclosure can comprise areduced amount of nornicotine of less than about 3 mg/g. For example,the nornicotine content in such a product can be 3.0 mg/g, 2.5 mg/g, 2.0mg/g, 1.5 mg/g, 1.0 mg/g, 750 μg/g, 500 μg/g, 250 μg/g, 100 μg/g, 75μg/g, 50 μg/g, 25 μg/g, 10 μg/g, 5 μg/g, 1 μg/g, 750 ng/g, 500 ng/g, 250ng/g, 100 ng/g, 75 ng/g, 50 ng/g, 25 ng/g, 10 ng/g, 5 ng/g, 1 ng/g, 750pg/g, 500 pg/g, 250 pg/g, 100 pg/g, 75 pg/g, 50 pg/g, 25 pg/g, 10 pg/g,7.0 pg/g, 5.0 pg/g, 4.0 pg/g, 2.0 pg/g, 1.0 pg/g, 0.5 pg/g, 0.4 pg/g,0.2 pg/g, 0.1 pg/g, 0.05 pg/g, 0.01 pg/g, and the like, or undetectable,or any range therein. The tobacco product typically has a reduced amountof NNN of less than about 10 pg/g. For example, the NNN content in sucha product can be about 10 pg/g, 7.0 pg/g, 5.0 pg/g, 4.0 pg/g, 2.0 pg/g,1.0 pg/g, 0.5 pg/g, 0.4 pg/g, 0.2 pg/g, 0.1 pg/g, 0.05 pg/g, 0.01 pg/g,and the like, or undetectable, or any range therein. The percentage ofsecondary alkaloids relative to total alkaloid content contained in aplant of the present disclosure may not be statistically different thanfrom a commercial seedlot of Banket A1, NC 2000, NC 2002, or Burley 21.

Differences between two inbred tobacco varieties or two hybrid tobaccovarieties can be evaluated using statistical approaches. Statisticalanalysis includes the calculation of mean values, determination of thestatistical significance of the sources of variation, and thecalculation of the appropriate variance components. Methods fordetermining statistical significance are known in the art. Statisticalsoftware is available, for example, the PROC GLM function of SAS.Significance is generally presented as a “p-value.” A statisticallysignificant p-value is less than 0.10. In a preferred aspect, thep-value is less than or equal to 0.05. In another aspect, the p-value is0.04 or less, 0.03 or less, or 0.02 or less. In yet another aspect, astatistically significant value is less than 0.01. In yet anotheraspect, it can be less than 0.009, less than 0.008, less than 0.007,less than 0.006, less than 0.005, less than 0.004, less than 0.003, lessthan 0.002, or less than 0.001.

Tobacco plants of the present disclosure that are homozygous for thecyp82e4 W329Stop, the cyp82e5v2 W422Stop, and cyp82e10 P381S alleleshave a reversion rate that is statistically significantly lower thancorresponding control low-converter plants having wild type nicotinedemethylase CYP82E4, E5, and E10 genes. In addition, homozygous CYP82E4,CYP82E5, and CYP82E10 triple mutant tobacco plants have a percentconversion to nornicotine of less than about 2.0%, e.g., undetectable toabout 2.0%, 1.9%, 1.8%, 1.7%, 1.6%, 1.5%, 1.4%, 1.3%, 1.2%, 1.1%, 1.0%,0.9%, 0.8%, or any range therein. In some aspects, the triple mutanttobacco plants have a percent conversion to nornicotine in a range from,for example, about 1.0% to 2.0%, 0.8% to 1.8%, 0.8% to 2.0%, or 1.0% to2.0%.

Nicotine and nornicotine can be measured in ethylene-treated leavesusing methods known in the art (e.g., gas chromatography). Percentnicotine demethylation in a sample is calculated by dividing the levelof nornicotine by the combined level of nicotine and nornicotine asmeasured in the sample, and multiplying by 100. Percent nicotinedemethylation in a sample from a plant of the present disclosure isabout 50, 40, 30, 20, or 10 percent of a sample from an individual plantgrown from a commercial seedlot of Banket A1, NC 2000, NC 2002, orBurley 21.

In an aspect, the tobacco plants of the present disclosure have a USDAquality index of about 73, about 72, about 71, about 70, about 69, about68, about 67 or about 66 or any range therein. In an aspect, the tobaccoplants of the present disclosure have a USDA quality index of about 65.In another aspect, the quality index may be at least about 55, 60, 62.5or greater, or any range therein. In another aspect, tobacco plants ofthe present disclosure can have a quality index in the range of about 60to about 65, about 60 to about 70, about 62.5 to about 65, about 62.5 toabout 70, or about 65 to about 70.

A plant of the present disclosure, including, but not limited to, BanketA1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002 SRC,CMS NC 2002 SRC, Burley 21 SRC, and CMS Burley 21 SRC, can have anyyield potential, including high (e.g., over 3000 lbs/A), moderately high(e.g., 2200-3000 lbs/A), and moderate (e.g., less than 2000 lbs/A) yieldpotential.

In another aspect, the present disclosure also provides for a plantgrown from the seed of a Banket A1 SRC or CMS Banket A1 SRC plant inwhich alkaloids obtained from tobacco plants grown for the seed havedecreased nornicotine, as well as plant parts and tissue cultures fromsuch plants, representative sample seeds of these cultivars having beendeposited with the ATCC, for example, under ATCC Accession No.PTA-121043 for Banket A1 SRC and ATCC Accession No. PTA-121054 for CMSBanket A1 SRC.

An aspect of the present disclosure provides for parts of the cultivarsBanket A1 SRC and CMS Banket A1 SRC. A part of a cultivar can compriseany plant part and includes, but is not limited to, leaves, pollen,embryos, cotyledons, hypocotyls, roots, root tips, anthers, flowers,ovules, shoots, stems, stalks, pith and capsules, tissue culturecomprising tissue, callus, cells or protoplasts of the cultivars BanketA1 SRC and CMS Banket A1 SRC. In another aspect, the present disclosureprovides for parts from hybrids of cultivars Banket A1 SRC and CMSBanket A1 SRC derived tobacco plants. In yet another aspect, the presentdisclosure provides for parts from genetically modified (e.g., byconventional breeding or genetic engineering techniques) forms of theforegoing plants and tissue culture.

In another aspect, the present disclosure also provides for a plantgrown from the seed of a NC 2000 SRC or CMS NC 2000 SRC plant in whichalkaloids obtained from tobacco plants grown for the seed have decreasednornicotine, as well as plant parts and tissue cultures from suchplants, representative sample seeds of these cultivars having beendeposited with the ATCC, for example, under ATCC Accession No.PTA-121053 for NC 2000 SRC and ATCC Accession No. PTA-121045 for CMS NC2000 SRC.

An aspect of the present disclosure provides for parts of the cultivarsNC 2000 SRC and CMS NC 2000 SRC. A part of a cultivar can comprise anyplant part and includes, but is not limited to, leaves, pollen, embryos,cotyledons, hypocotyls, roots, root tips, anthers, flowers, ovules,shoots, stems, stalks, pith and capsules, tissue culture comprisingtissue, callus, cells or protoplasts of the cultivars NC 2000 SRC andCMS NC 2000 SRC. In another aspect, the present disclosure provides forparts from hybrids of cultivars NC 2000 SRC and CMS NC 2000 SRC derivedtobacco plants. In yet another aspect, the present disclosure providesfor parts from genetically modified (e.g., by conventional breeding orgenetic engineering techniques) forms of the foregoing plants and tissueculture.

In another aspect, the present disclosure also provides for a plantgrown from the seed of a NC 2002 SRC or CMS NC 2002 SRC plant in whichalkaloids obtained from tobacco plants grown for the seed have decreasednornicotine, as well as plant parts and tissue cultures from suchplants, representative sample seeds of these cultivars having beendeposited with the ATCC, for example, under ATCC Accession No.PTA-121058 for NC 2002 SRC and ATCC Accession No. PTA-121041 for CMS NC2002 SRC.

An aspect of the present disclosure provides for parts of the cultivarsNC 2002 SRC and CMS NC 2002 SRC. A part of a cultivar can comprise anyplant part and includes, but is not limited to, leaves, pollen, embryos,cotyledons, hypocotyls, roots, root tips, anthers, flowers, ovules,shoots, stems, stalks, pith and capsules, tissue culture comprisingtissue, callus, cells or protoplasts of the cultivars NC 2002 SRC andCMS NC 2002 SRC. In another aspect, the present disclosure provides forparts from hybrids of cultivars NC 2002 SRC and CMS NC 2002 SRC derivedtobacco plants. In yet another aspect, the present disclosure providesfor parts from genetically modified (e.g., by conventional breeding orgenetic engineering techniques) forms of the foregoing plants and tissueculture.

In another aspect, the present disclosure also provides for a plantgrown from the seed of a Burley 21 SRC or CMS Burley 21 SRC plant inwhich alkaloids obtained from tobacco plants grown for the seed havedecreased nornicotine, as well as plant parts and tissue cultures fromsuch plants, representative sample seeds of these cultivars having beendeposited with the ATCC, for example, under ATCC Accession No.PTA-121059 for Burley 21 SRC and ATCC Accession No. PTA-121044 for CMSBurley 21 SRC.

An aspect of the present disclosure provides for parts of the cultivarsBurley 21 SRC and CMS Burley 21 SRC. A part of a cultivar can compriseany plant part and includes, but is not limited to, leaves, pollen,embryos, cotyledons, hypocotyls, roots, root tips, anthers, flowers,ovules, shoots, stems, stalks, pith and capsules, tissue culturecomprising tissue, callus, cells or protoplasts of the cultivars Burley21 SRC and CMS Burley 21 SRC. In another aspect, the present disclosureprovides for parts from hybrids of cultivars Burley 21 SRC and CMSBurley 21 SRC derived tobacco plants. In yet another aspect, the presentdisclosure provides for parts from genetically modified (e.g., byconventional breeding or genetic engineering techniques) forms of theforegoing plants and tissue culture.

Additional aspects of the present disclosure provide products comprisingtobacco from the plants of the present disclosure, and parts thereof.Other aspects of the disclosure provide cured plant parts, whichinclude, but are not limited to, a leaf, pollen, ovule, embryo,cotyledon, hypocotyl, meristematic cell, protoplast, root, root tip,pistil, anther, flower, shoot, stem, pod, petiole, and the like, andcombinations thereof.

Thus, in some aspects, the present disclosure provides a cured tobaccocomprising the leaves of the tobacco plant designated Banket A1 SRC, arepresentative sample seed of said cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121043. In another aspect, thepresent disclosure provides a cured tobacco comprising the leaves of thetobacco plant designated CMS Banket A1 SRC, a representative sample seedof said cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121054.

In an aspect, the present disclosure provides a cured tobacco comprisingthe stems of the tobacco plant designated Banket A1 SRC, arepresentative sample seed of said cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121043. In another aspect, thepresent disclosure provides a cured tobacco comprising the stems of thetobacco plant designated CMS Banket A1 SRC, a representative sample seedof said cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121054.

In an aspect, the present disclosure provides a cured tobacco comprisingthe leaves and stems of the tobacco plants designated Banket A1 SRC, arepresentative sample seed of said cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121043. In another aspect, thepresent disclosure provides a cured tobacco comprising the leaves andstems of the tobacco plants designated CMS Banket A1 SRC, arepresentative sample seed of said cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121054.

In some aspects, the present disclosure provides a cured tobaccocomprising the leaves of the tobacco plant designated NC 2000 SRC, arepresentative sample seed of said cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121053. In another aspect, thepresent disclosure provides a cured tobacco comprising the leaves of thetobacco plant designated CMS NC 2000 SRC, a representative sample seedof said cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121045.

In an aspect, the present disclosure provides a cured tobacco comprisingthe stems of the tobacco plant designated NC 2000 SRC, a representativesample seed of said cultivar having been deposited with the ATCC underATCC Accession No. PTA-121053. In another aspect, the present disclosureprovides a cured tobacco comprising the stems of the tobacco plantdesignated CMS NC 2000 SRC, a representative sample seed of saidcultivar having been deposited with the ATCC under ATCC Accession No.PTA-121045.

In an aspect, the present disclosure provides a cured tobacco comprisingthe leaves and stems of the tobacco plants designated NC 2000 SRC, arepresentative sample seed of said cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121053. In another aspect, thepresent disclosure provides a cured tobacco comprising the leaves andstems of the tobacco plants designated CMS NC 2000 SRC, a representativesample seed of said cultivar having been deposited with the ATCC underATCC Accession No. PTA-121045.

In some aspects, the present disclosure provides a cured tobaccocomprising the leaves of the tobacco plant designated NC 2002 SRC, arepresentative sample seed of said cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121058. In another aspect, thepresent disclosure provides a cured tobacco comprising the leaves of thetobacco plant designated CMS NC 2002 SRC, a representative sample seedof said cultivar having been deposited with the ATCC under ATCCAccession No. PTA-121041.

In an aspect, the present disclosure provides a cured tobacco comprisingthe stems of the tobacco plant designated NC 2002 SRC, a representativesample seed of said cultivar having been deposited with the ATCC underATCC Accession No. PTA-121058. In another aspect, the present disclosureprovides a cured tobacco comprising the stems of the tobacco plantdesignated CMS NC 2002 SRC, a representative sample seed of saidcultivar having been deposited with the ATCC under ATCC Accession No.PTA-121041.

In an aspect, the present disclosure provides a cured tobacco comprisingthe leaves and stems of the tobacco plants designated NC 2002 SRC, arepresentative sample seed of said cultivar having been deposited withthe ATCC under ATCC Accession No. PTA-121058. In another aspect, thepresent disclosure provides a cured tobacco comprising the leaves andstems of the tobacco plants designated CMS NC 2002 SRC, a representativesample seed of said cultivar having been deposited with the ATCC underATCC Accession No. PTA-121041.

The present disclosure also provides a container of Banket A1 SRC or CMSBanket A1 SRC seeds or other seeds of the present disclosure in whichalkaloids obtained from tobacco plants grown from greater than about 50%of the seeds have decreased nornicotine.

In another aspect, alkaloids obtained from Banket A1 SRC or CMS BanketA1 SRC plants or other plants of the present disclosure grown fromgreater than about 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95% of theseeds in the container have decreased nornicotine, representativesamples of seeds of these cultivars having been deposited with the ATCC,for example, under ATCC Accession No. PTA-121043 for Banket A1 SRCand/or ATCC Accession No. PTA-121054 for CMS Banket A1 SRC.

The container of Banket A1 SRC or CMS Banket A1 SRC seeds or other seedsof the present disclosure may contain any number, weight or volume ofseeds. For example, a container can contain at least, or greater than,about 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1500, 2000,2500, 3000, 3500, 4000 or more seeds. Alternatively, the container cancontain at least, or greater than, about 1 ounce, 5 ounces, 10 ounces, 1pound, 2 pounds, 3 pounds, 4 pounds, 5 pounds or more seeds.Representative samples of seeds of these cultivars having been depositedwith the ATCC, for example, under ATCC Accession No. PTA-121043 forBanket A1 SRC and/or ATCC Accession No. PTA-121054 for CMS Banket A1SRC.

Containers of Banket A1 SRC or CMS Banket A1 SRC seeds or other seeds ofthe present disclosure may be any container available in the art. By wayof a non-limiting example, a container may be a box, a bag, a packet, apouch, a tape roll, a pail, a foil, or a tube. Representative samples ofseeds of these cultivars having been deposited with the ATCC, forexample, under ATCC Accession No. PTA-121043 for Banket A1 SRC and/orATCC Accession No. PTA-121054 for CMS Banket A1 SRC.

In another aspect, the present disclosure also provides a container ofBanket A1 SRC or CMS Banket A1 SRC in which greater than about 50% ofBanket A1 SRC or CMS Banket A1 SRC seeds or other seeds of the presentdisclosure have decreased nornicotine. Representative samples of seedsof these cultivars having been deposited with the ATCC, for exampleunder ATCC Accession No. PTA-121043 for Banket A1 SRC and/or ATCCAccession No. PTA-121054 for CMS Banket A1 SRC.

In one aspect, the present disclosure provides a seed of a Banket A1 SRCor CMS Banket A1 SRC plant or other plant of the present disclosure inwhich a plant grown from said seed is male sterile. Representativesamples of seeds of these cultivars having been deposited with the ATCC,for example, under ATCC Accession No. PTA-121043 for Banket A1 SRCand/or ATCC Accession No. PTA-121054 for CMS Banket A1 SRC.

The present disclosure also provides a container of NC 2000 SRC or CMSNC 2000 SRC seeds or other seeds of the present disclosure in whichalkaloids obtained from tobacco plants grown from greater than about 50%of the seeds have decreased nornicotine. In another aspect, alkaloidsobtained from NC 2000 SRC or CMS NC 2000 SRC plants or other plants ofthe present disclosure grown from greater than about 55%, 60%, 65%, 70%,75%, 80%, 85%, 90%, or 95% of the seeds in the container have decreasednornicotine, representative samples of seeds of these cultivars havingbeen deposited with the ATCC, for example, under ATCC Accession No.PTA-121053 for NC 2000 SRC and/or ATCC Accession No. PTA-121045 for CMSNC 2000 SRC.

The container of NC 2000 SRC or CMS NC 2000 SRC seeds or other seeds ofthe present disclosure may contain any number, weight or volume ofseeds. For example, a container can contain at least, or greater than,about 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1500, 2000,2500, 3000, 3500, 4000 or more seeds. Alternatively, the container cancontain at least, or greater than, about 1 ounce, 5 ounces, 10 ounces, 1pound, 2 pounds, 3 pounds, 4 pounds, 5 pounds or more seeds.Representative samples of seeds of these cultivars having been depositedwith the ATCC, for example, under ATCC Accession No. PTA-121053 for NC2000 SRC and/or ATCC Accession No. PTA-121045 for CMS NC 2000 SRC.

Containers of NC 2000 SRC or CMS NC 2000 SRC seeds or other seeds of thepresent disclosure may be any container available in the art. By way ofa non-limiting example, a container may be a box, a bag, a packet, apouch, a tape roll, a pail, a foil, or a tube. Representative samples ofseeds of these cultivars having been deposited with the ATCC, forexample, under ATCC Accession No. PTA-121053 for NC 2000 SRC and/or ATCCAccession No. PTA-121045 for CMS NC 2000 SRC.

In another aspect, the present disclosure also provides a container ofNC 2000 SRC or CMS NC 2000 SRC in which greater than about 50% of NC2000 SRC or CMS NC 2000 SRC seeds or other seeds of the presentdisclosure have decreased nornicotine. Representative samples of seedsof these cultivars having been deposited with the ATCC, for exampleunder ATCC Accession No. PTA-121053 for NC 2000 SRC and/or ATCCAccession No. PTA-121045 for CMS NC 2000 SRC.

In one aspect, the present disclosure provides a seed of a NC 2000 SRCor CMS NC 2000 SRC plant or other plant of the present disclosure inwhich a plant grown from said seed is male sterile. Representativesamples of seeds of these cultivars having been deposited with the ATCC,for example, under ATCC Accession No. PTA-121053 for NC 2000 SRC and/orATCC Accession No. PTA-121045 for CMS NC 2000 SRC.

The present disclosure also provides a container of NC 2002 SRC or CMSNC 2002 SRC seeds or other seeds of the present disclosure in whichalkaloids obtained from tobacco plants grown from greater than about 50%of the seeds have decreased nornicotine. In another aspect, alkaloidsobtained from NC 2002 SRC or CMS NC 2002 SRC plants or other plants ofthe present disclosure grown from greater than about 55%, 60%, 65%, 70%,75%, 80%, 85%, 90%, or 95% of the seeds in the container have decreasednornicotine, representative samples of seeds of these cultivars havingbeen deposited with the ATCC, for example, under ATCC Accession No.PTA-121058 for NC 2002 SRC and/or ATCC Accession No. PTA-121041 for CMSNC 2002 SRC.

The container of NC 2002 SRC or CMS NC 2002 SRC seeds or other seeds ofthe present disclosure may contain any number, weight or volume ofseeds. For example, a container can contain at least, or greater than,about 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1500, 2000,2500, 3000, 3500, 4000 or more seeds. Alternatively, the container cancontain at least, or greater than, about 1 ounce, 5 ounces, 10 ounces, 1pound, 2 pounds, 3 pounds, 4 pounds, 5 pounds or more seeds.Representative samples of seeds of these cultivars having been depositedwith the ATCC, for example, under ATCC Accession No. PTA-121058 for NC2002 SRC and/or ATCC Accession No. PTA-121041 for CMS NC 2002 SRC.

Containers of NC 2002 SRC or CMS NC 2002 SRC seeds or other seeds of thepresent disclosure may be any container available in the art. By way ofa non-limiting example, a container may be a box, a bag, a packet, apouch, a tape roll, a pail, a foil, or a tube. Representative samples ofseeds of these cultivars having been deposited with the ATCC, forexample, under ATCC Accession No. PTA-121058 for NC 2002 SRC and/or ATCCAccession No. PTA-121041 for CMS NC 2002 SRC.

In another aspect, the present disclosure also provides a container ofNC 2002 SRC or CMS NC 2002 SRC in which greater than about 50% of NC2002 SRC or CMS NC 2002 SRC seeds or other seeds of the presentdisclosure have decreased nornicotine. Representative samples of seedsof these cultivars having been deposited with the ATCC, for exampleunder ATCC Accession No. PTA-121058 for NC 2002 SRC and/or ATCCAccession No. PTA-121041 for CMS NC 2002 SRC.

In one aspect, the present disclosure provides a seed of a NC 2002 SRCor CMS NC 2002 SRC plant or other plant of the present disclosure inwhich a plant grown from said seed is male sterile. Representativesamples of seeds of these cultivars having been deposited with the ATCC,for example, under ATCC Accession No. PTA-121058 for NC 2002 SRC and/orATCC Accession No. PTA-121041 for CMS NC 2002 SRC.

The present disclosure also provides a container of Burley 21 SRC or CMSBurley 21 SRC seeds or other seeds of the present disclosure in whichalkaloids obtained from tobacco plants grown from greater than about 50%of the seeds have decreased nornicotine. In another aspect, alkaloidsobtained from Burley 21 SRC or CMS Burley 21 SRC plants or other plantsof the present disclosure grown from greater than about 55%, 60%, 65%,70%, 75%, 80%, 85%, 90%, or 95% of the seeds in the container havedecreased nornicotine, representative samples of seeds of thesecultivars having been deposited with the ATCC, for example, under ATCCAccession No. PTA-121059 for Burley 21 SRC and/or ATCC Accession No.PTA-121044 for CMS Burley 21 SRC.

The container of Burley 21 SRC or CMS Burley 21 SRC seeds or other seedsof the present disclosure may contain any number, weight or volume ofseeds. For example, a container can contain at least, or greater than,about 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1500, 2000,2500, 3000, 3500, 4000 or more seeds. Alternatively, the container cancontain at least, or greater than, about 1 ounce, 5 ounces, 10 ounces, 1pound, 2 pounds, 3 pounds, 4 pounds, 5 pounds or more seeds.Representative samples of seeds of these cultivars having been depositedwith the ATCC, for example, under ATCC Accession No. PTA-121059 forBurley 21 SRC and/or ATCC Accession No. PTA-121044 for CMS Burley 21SRC.

Containers of Burley 21 SRC or CMS Burley 21 SRC seeds or other seeds ofthe present disclosure may be any container available in the art. By wayof a non-limiting example, a container may be a box, a bag, a packet, apouch, a tape roll, a pail, a foil, or a tube. Representative samples ofseeds of these cultivars having been deposited with the ATCC, forexample, under ATCC Accession No. PTA-121059 for Burley 21 SRC and/orATCC Accession No. PTA-121044 for CMS Burley 21 SRC.

In another aspect, the present disclosure also provides a container ofBurley 21 SRC or CMS Burley 21 SRC in which greater than about 50% ofBurley 21 SRC or CMS Burley 21 SRC seeds or other seeds of the presentdisclosure have decreased nornicotine. Representative samples of seedsof these cultivars having been deposited with the ATCC, for exampleunder ATCC Accession No. PTA-121059 for Burley 21 SRC and/or ATCCAccession No. PTA-121044 for CMS Burley 21 SRC.

In one aspect, the present disclosure provides a seed of a Burley 21 SRCor CMS Burley 21 SRC plant or other plant of the present disclosure inwhich a plant grown from said seed is male sterile. Representativesamples of seeds of these cultivars having been deposited with the ATCC,for example, under ATCC Accession No. PTA-121059 for Burley 21 SRCand/or ATCC Accession No. PTA-121044 for CMS Burley 21 SRC.

Tobacco material obtained from the tobacco lines, varieties or hybridsof the present disclosure can be used to make tobacco productsincluding, without limitation, cigarette products (e.g., cigarettes andbidi cigarettes), cigar products (e.g., cigar wrapping tobacco andcigarillos), pipe tobacco products, smokeless cigarette products,smokeless tobacco products (e.g., moist snuff, dry snuff, and chewingtobacco), films, chewables, tabs, shaped parts, gels, consumable units,insoluble matrices, hollow shapes and the like. See, e.g., U.S. PatentPublication No. US 2006/0191548, which is herein incorporated byreference in its entirety.

Tobacco products derived from plants of the present disclosure alsoinclude cigarettes and other smoking articles, particularly thosesmoking articles including filter elements, wherein the rod of smokeablematerial includes cured tobacco within a tobacco blend. In an aspect, atobacco product can include but is not limited to pipe tobacco, cigartobacco, cigarette tobacco, chewing tobacco, leaf tobacco, shreddedtobacco, and/or cut tobacco or any combination thereof.

In an aspect, the tobacco product of the present disclosure can be ablended tobacco product. In other aspects of the disclosure, the tobaccoproduct of the present disclosure can be a reduced nicotine tobaccoproduct. In still other aspects, the tobacco product of the presentdisclosure can be a blended tobacco product with reduced nicotinecontent. Thus, the tobacco product of the present disclosure can be ablended reduced nicotine tobacco product. Tobacco product materialcomprises a blend of tobacco materials from the present disclosure,wherein the blend comprises at least about 5, 10, 20, 30, 40, 50, 60,70, 80, 90, or 95 percent by weight of a cured tobacco, or any rangetherein, based on the dry weight of the tobacco material. US2008/0245377 is herein incorporated by reference for blend mixtures inits entirety.

In an aspect, tobacco products having a reduced amount of nitrosaminecontent can be manufactured using tobacco plant material from plants andplant parts of the present disclosure. Thus, in some aspects, a tobaccoproduct manufactured using tobacco plant material from plants and plantparts of the present disclosure can comprise a reduced amount ofnornicotine of less than about 3 mg/g. For example, the nornicotinecontent in such a product can be 3.0 mg/g, 2.5 mg/g, 2.0 mg/g, 1.5 mg/g,1.0 mg/g, 750 μg/g, 500 pg/g, 250 μg/g, 100 pg/g, 75 pg/g, 50 μg/g, 25μg/g, 10 μg/g, 5 μg/g, 1 μg/g, 750 ng/g, 500 ng/g, 250 ng/g, 100 ng/g,75 ng/g, 50 ng/g, 25 ng/g, 10 ng/g, 5 ng/g, 1 ng/g, 750 pg/g, 500 pg/g,250 pg/g, 100 pg/g, 75 pg/g, 50 pg/g, 25 pg/g, 10 pg/g, 7.0 pg/g, 5.0pg/g, 4.0 pg/g, 2.0 pg/g, 1.0 pg/g, 0.5 pg/g, 0.4 pg/g, 0.2 pg/g, 0.1pg/g, 0.05 pg/g, 0.01 pg/g, or undetectable, or any range therein. Thetobacco product typically has a reduced amount of NNN of less than about10 pg/g. For example, the NNN content in such a product can be about 10pg/g, 7.0 pg/g, 5.0 pg/g, 4.0 pg/g, 2.0 pg/g, 1.0 pg/g, 0.5 pg/g, 0.4pg/g, 0.2 pg/g, 0.1 pg/g, 0.05 pg/g, 0.01 pg/g, or undetectable, or anyrange therein. The percentage of secondary alkaloids relative to totalalkaloid content contained in a plant of the present disclosure may notbe statistically different than from a commercial seedlot of Banket A1,NC 2000, NC 2002, or Burley 21.

A tobacco plant of the present disclosure designated Banket A1 SRC, CMSBanket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002 SRC, CMS NC 2002SRC, Burley 21 SRC, or CMS Burley 21 SRC carrying the cyp82e4 W329Stop,the cyp82e5v2 W422Stop, and cyp82e10 P381S alleles can be used in aplant breeding program to create useful lines, cultivars, varieties,progeny, inbreds, and hybrids. Thus, in some aspects, an F₁, F₂, F₃, orlater generation tobacco plant containing the cyp82e4 W329Stop, thecyp82e5v2 W422Stop, and cyp82e10 P381S alleles is crossed with a secondNicotiana plant, and progeny of the cross are identified in which thecyp82e4 W329Stop, the cyp82e5v2 W422Stop, and cyp82e10 P381 S allelesare present. It will be appreciated that the second Nicotiana plant canbe Banket A1, NC 2000, NC 2002, Burley 21 or any other Nicotiana speciesor line, optionally with an additional desirable trait, such asherbicide resistance.

In still other aspects, methods of the present disclosure furtherinclude self-pollinating or pollinating a male sterile pollen acceptorwith a pollen donor capable of being used in production of a progenyplant of the present disclosure, such as a male sterile hybrid of thepresent disclosure. Either the male sterile pollen acceptor plant or thepollen donor plant has at least one mutant allele, two, or even threemutant alleles at a nicotine demethylase locus, such as the cyp82e4W329Stop allele, the cyp82e5v2 W422Stop allele, and/or the cyp82e10P381S allele. In an aspect, all three alleles at each nicotinedemethylase locus are mutant alleles, making the plant homozygous forcyp82e4 W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S.

Breeding can be carried out via any known procedures. DNAfingerprinting, SNP or similar technologies may be used in amarker-assisted selection (MAS) breeding program to transfer or breedmutant alleles of a nicotine demethylase gene into other tobaccos. Forexample, a breeder can create segregating populations fromhybridizations of a genotype containing cyp82e4 W329Stop, cyp82e5v2W422Stop, and cyp82e10 P381 S alleles with an agronomically desirablegenotype. Plants in the F₂ or backcross generations can be screenedusing a marker developed from cyp82e4 W329Stop, cyp82e5v2 W422Stop, orcyp82e10 P381S alleles or a fragment thereof, using one of thetechniques known in the art or disclosed herein. Plants identified aspossessing one or more cyp82e4 W329Stop, cyp82e5v2 W422Stop, andcyp82e10 P381S alleles can be backcrossed or self-pollinated to create asecond population to be screened. Depending on the expected inheritancepattern or the MAS technology used, it may be necessary toself-pollinate the selected plants before each cycle of backcrossing toaid identification of the desired individual plants. Backcrossing orother breeding procedure can be repeated until the desired phenotype ofthe recurrent parent is recovered. A recurrent parent in the presentdisclosure can be Banket A1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC2000 SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley 21 SRC, or CMS Burley 21SRC. Other breeding techniques can be found, for example, in Wernsman,E. A., and Rufty, R. C., 1987. Chapter Seventeen. Tobacco. pages 669-698In: Cultivar Development. Crop Species. W. H. Fehr (ed.), MacMillanPublishing Go., Inc., New York, N.Y., incorporated herein by referencein their entireties.

Nicotiana species which exhibit breeding compatibility with Nicotianatabacum include Nicotiana amplexicaulis, PI 271989; Nicotianabenthamiana PI 555478; Nicotiana bigelovii PI 555485; Nicotiana debneyi;Nicotiana excelsior PI 224063; Nicotiana glutinosa PI 555507; Nicotianagoodspeedii PI 241012; Nicotiana gossei PI 230953; Nicotiana hesperis PI271991; Nicotiana knightiana PI 555527; Nicotiana maritima PI 555535;Nicotiana megalosiphon PI 555536; Nicotiana nudicaulis PI 555540;Nicotiana paniculata PI 555545; Nicotiana plumbaginifolia PI 555548;Nicotiana repanda PI 555552; Nicotiana rustica; Nicotiana suaveolens PI230960; Nicotiana sylvestris PI 555569; Nicotiana tomentosa PI 266379;Nicotiana tomentosiformis; and Nicotiana trigonophylla PI 555572. Seealso, Compendium of Tobacco Diseases published by AmericanPhytopathology Society, or The Genus Nicotiana Illustrated, published byJapan Tobacco Inc, hereby incorporated by reference in their entireties.

The result of a plant breeding program using the mutant tobacco plantsdescribed herein includes useful lines, cultivars, varieties, progeny,inbreds, and hybrids. As used herein, the term “cultivar” or “variety”refers to a population of plants that share constant characteristicswhich separate them from other plants of the same species. A cultivar orvariety is often, although not always, sold commercially. Whilepossessing one or more distinctive traits, a cultivar or variety isfurther characterized by a very small overall variation betweenindividuals within that cultivar or variety. A “pure line” variety maybe created by several generations of self-pollination and selection, orvegetative propagation from a single parent using tissue or cell culturetechniques. A cultivar or variety can be essentially derived fromanother cultivar, line, or variety. As defined by the InternationalConvention for the Protection of New Varieties of Plants (Dec. 2, 1961,as revised at Geneva on Nov. 10, 1972, on Oct. 23, 1978, and on Mar. 19,1991), a cultivar or variety is “essentially derived” from an initialcultivar or variety if: a) it is predominantly derived from the initialcultivar or variety, or from a cultivar or variety that is predominantlyderived from the initial cultivar or variety, while retaining theexpression of the essential characteristics that result from thegenotype or combination of genotypes of the initial cultivar or variety;b) it is clearly distinguishable from the initial cultivar or variety;and c) except for the differences which result from the act ofderivation, it conforms to the initial cultivar or variety in theexpression of the essential characteristics that result from thegenotype or combination of genotypes of the initial cultivar or variety.Essentially derived varieties can be obtained, for example, by theselection of a natural or induced mutant, a somaclonal variant, avariant individual from plants of the initial cultivar or variety,backcrossing, or transformation. A “line” as distinguished from acultivar or variety most often denotes a group of plants usednon-commercially, for example in plant research. A line typicallydisplays little overall variation between individuals for one or moretraits of interest, although there may be some variation betweenindividuals for other traits.

Hybrid tobacco varieties can be produced by preventing self-pollinationof female parent plants (i.e., seed parents) of a first variety,permitting pollen from male parent plants of a second variety tofertilize the female parent plants, and allowing F₁ hybrid seeds to formon the female plants. Self-pollination of female plants can be preventedby emasculating the flowers at an early stage of flower development.Alternatively, pollen formation can be prevented on the female parentplants using a form of male sterility. For example, male sterility canbe produced by cytoplasmic male sterility (CMS), or transgenic malesterility wherein a transgene inhibits microsporogenesis and/or pollenformation, or self-incompatibility. Female parent plants containing CMSare particularly useful. In aspects in which the female parent plantsare CMS, pollen may be harvested from male fertile plants and appliedmanually to the stigmas of CMS female parent plants, and the resultingF₁ seed is harvested.

Plants can be used to form single-cross tobacco F₁ hybrids. In such anaspect, the plants of the parent varieties can be grown as substantiallyhomogeneous adjoining populations to facilitate naturalcross-pollination from the male parent plants to the female parentplants. The F₁ seed formed on the female parent plants is selectivelyharvested by conventional means. One also can grow the two parent plantvarieties in bulk and harvest a blend of F₁ hybrid seed formed on thefemale parent and seed formed upon the male parent as the result ofself-pollination. Alternatively, three-way crosses can be carried outwherein a single-cross F₁ hybrid is used as a female parent and iscrossed with a different male parent. As another alternative,double-cross hybrids can be created wherein the F₁ progeny of twodifferent single-crosses are themselves crossed. Self-incompatibilitycan be used to particular advantage to prevent self-pollination offemale parents when forming a double-cross hybrid.

Successful crosses yield F₁ plants that are fertile, have cyp82e4W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S alleles, and can bebackcrossed with one of the parents, such as Banket A1 SRC, CMS BanketA1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002 SRC, CMS NC 2002 SRC,Burley 21 SRC, or CMS Burley 21 SRC, if desired. In some aspects, aplant population in the F₂ generation is screened for cyp82e4 W329Stop,the cyp82e5v2 W422Stop, and cyp82e10 P381S alleles. Selected plants canbe crossed with one of the parents and the first backcross (BC1)generation plants are self-pollinated to produce a BC1 F₂ populationthat is again screened for variant nicotine demethylase gene expression(e.g., the null version of the nicotine demethylase gene). The processof backcrossing, self-pollination, and screening is repeated, forexample, at least four times, until the final screening produces a plantthat is fertile and reasonably similar to the recurrent parent. Thisplant, if desired, is self-pollinated and the progeny are subsequentlyscreened again to confirm that the plant exhibits the same low nicotineconversion phenotype as Banket A1 SRC, CMS Banket A1 SRC, NC 2000 SRC,CMS NC 2000 SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley 21 SRC, or CMSBurley 21 SRC. Breeder's seed of the selected plant is produced usingstandard methods including, for example, field testing, confirmation ofthe null condition for nicotine demethylase, chemical analyses of curedleaf to determine the level of alkaloids and/or chemical analyses ofcured leaf to determine the ratio of nornicotine tonicotine+nornicotine.

In one aspect, an F₁ progeny is the result of a cross between Banket A1SRC and CMS Banket A1 SRC, between NC 2000 SRC and CMS NC 2000 SRC,between NC 2002 SRC and CMS NC 2002 SRC, or between Burley 21 SRC andCMS Burley 21 SRC to generate F₁ progeny that are male sterile. Malesterile tobacco plants may be produced by any method known in the art.Methods of producing male sterile tobacco are described in Wernsman, E.A., and Rufty, R. C., 1987. Chapter Seventeen. Tobacco. pages 669-698In: Cultivar Development. Crop Species. W. H. Fehr (ed.), MacMillanPublishing Go., Inc., New York, N.Y. 761 pp.

The present disclosure further provides methods of producing a tobaccoplant by crossing one of cultivars Banket A1 SRC, CMS Banket A1 SRC, NC2000 SRC, CMS NC 2000 SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley 21 SRC,or CMS Burley 21 SRC with itself or a different tobacco line. Thedisclosure further relates to methods for producing other tobaccocultivars or breeding lines derived from cultivars Banket A1 SRC, CMSBanket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002 SRC, CMS NC 2002SRC, Burley 21 SRC, or CMS Burley 21 SRC by crossing a plant ofcultivars Banket A1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC 2000SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley 21 SRC, or CMS Burley 21 SRCwith a second tobacco plant and growing the progeny seed to yield aBanket A1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002SRC, CMS NC 2002 SRC, Burley 21 SRC, or CMS Burley 21 SRC—derivedtobacco plant. An additional aspect of the present disclosure provides amethod for producing a tobacco plant that contains in its geneticmaterial one or more transgenes, comprising crossing cultivars of thepresent disclosure with a second cultivar containing one or moretransgenes wherein progeny are produced, so that the genetic material ofthe progeny that result from the cross comprise the transgene(s)optionally operably linked to one or more regulatory elements. In oneaspect, the second cultivar may be a plant derived from cultivars BanketA1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002 SRC,CMS NC 2002 SRC, Burley 21 SRC, or CMS Burley 21 SRC transformed withone or more transgenes.

The disclosure further provides for the vegetative propagation of aplant of cultivars Banket A1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC2000 SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley 21 SRC, or CMS Burley 21SRC and progeny thereof. In one aspect, the disclosure provides for amethod of vegetatively propagating a plant of a tobacco cultivarcomprising collecting tissue capable of being propagated from a plant ofcultivars Banket A1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC 2000SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley 21 SRC, or CMS Burley 21 SRC,cultivating the tissue to obtain a proliferated shoot and rooting theproliferated shoots to obtain a rooted plantlet. In another aspect, theplant tissue may be collected from an F₁ hybrid of a plant of cultivarsBanket A1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002SRC, CMS NC 2002 SRC, Burley 21 SRC, or CMS Burley 21 SRC. In an aspect,the plant tissue may be collected from an F₂, F₃, F₄ or later progenyplant obtained by breeding a plant of cultivars Banket A1 SRC, CMSBanket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002 SRC, CMS NC 2002SRC, Burley 21 SRC, or CMS Burley 21 SRC.

A plant comprising a mutation in a nicotine demethylase gene can beidentified by selecting or screening the mutagenized plant material, orprogeny thereof. Such screening and selection methodologies are known tothose having ordinary skill in the art. Examples of screening andselection methodologies include, but are not limited to, Southernanalysis, PCR amplification for detection of a polynucleotide, Northernblots, RNase protection, primer-extension, RT-PCR amplification fordetecting RNA transcripts, enzymatic assays for detecting enzyme orribozyme activity of polypeptides and polynucleotides, and protein gelelectrophoresis, Western blots, immunoprecipitation, and enzyme-linkedimmunoassays to detect polypeptides. Other techniques such as in situhybridization, enzyme staining, and immunostaining also can be used todetect the presence or expression of polypeptides and/orpolynucleotides. Methods for performing all of the referenced techniquesare known.

It is understood that a tobacco plant of the present disclosure,including, but not limited to, Banket A1 SRC, CMS Banket A1 SRC, NC 2000SRC, CMS NC 2000 SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley 21 SRC, andCMS Burley 21 SRC, can be transformed by a genetic construct (nucleicacid construct) or transgene using any technique known in the art.Without limitation, an example of a desired trait can include herbicideresistance, pest resistance, disease resistance, high yield, high gradeindex, curability, curing quality, mechanical harvestability, holdingability, leaf quality, height, plant maturation (e.g., early maturing,early to medium maturing, medium maturing, medium to late maturing, orlate maturing), stalk size (e.g., small, medium, or large stalk), orleaf number per plant (e.g., small (e.g., 5-10 leaves), medium (e.g.,11-15 leaves), or large (e.g., 16-21) number of leaves), or anycombination. Any plant of the present disclosure can be used as a basisfor tissue culture, regeneration, transformed, or a combination of anyof these. In an aspect, a plant of the present disclosure derived bytissue culture, transformation, or both has all, or essentially all, ofthe morphological and physiological characteristics of cultivar BanketA1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002 SRC,CMS NC 2002 SRC, Burley 21 SRC, or CMS Burley 21 SRC.

Having now generally described the disclosure, the same will be morereadily understood through reference to the following examples that areprovided by way of illustration, and are not intended to be limiting ofthe present disclosure, unless specified.

EXAMPLES Example 1 Breeding of Homozygous cyp82e4 W329Stop, thecyp82e5v2 W422Stop, and cyp82e10 P381S Mutant Plants into the Banket A1Burley Tobacco Cultivar

Banket A1 SRC is a backcross-derived version of burley tobacco cultivarBanket A1 carrying introduced mutations in three genes (CYP82E4 (SEQ IDNO: 5), CYP82E5 (SEQ ID NO: 8), and CYP82E10 (SEQ ID NO: 9)) previouslydocumented to encode for nicotine demethylase enzymes (Lewis et al.,Phytochemistry, 71 (2010), 1988-1998). The introduced mutations inCYP82E4 (cyp82e4 W329Stop (SEQ ID NO: 1)) and CYP82E5 (cyp82e5v2W422Stop (SEQ ID NO: 2)) encode for premature stop codons which renderthe genes non-functional. The introduced mutation in CYP82E10 (cyp82e10P381S (SEQ ID NO: 11)) does not encode for a premature stop codon, butdoes render the gene product non-functional for converting nicotine tonornicotine (Id.). When in homozygous condition, the three mutationsresult in tobacco plants with (1) reduced genetic capacity todemethylate nicotine to form nornicotine, and (2) a correspondingdiminished potential to accumulate N-nitrosonornicotine (NNN), a potentcarcinogen found in many tobacco products. The mutation in CYP82E4(cyp82e4 W329Stop (SEQ ID NO: 1)) also provides phenotypic stability forthe “nicotine conversion” trait and eliminates the requirement toutilize the cumbersome “LC” method for reducing levels of nornicotine intobacco cultivars (see e.g., Jack et al. 2007. Implications of reducingnornicotine accumulation in burley tobacco: appendix A—the LC protocol.Rec. Adv. Tob. Sci. 33: 58-79).

The original tobacco cultivar Banket A1 is a fertile inbred line. CMSBanket A1 is a cytoplasmic male-sterile version of Banket A1. To developBanket A1 SRC, an individual plant of Banket A1 is pollinated with aplant of the genetic background DH98-325-6 carrying mutations in each ofthe three nicotine demethylase genes. F₁ individuals originating fromthis cross and heterozygous for each mutation are backcrossed to therecurrent parent, Banket A1, to produce BC₁F₁ progeny. BC₁F₁ progeny arescreened using genotyping methodologies to identify individualsheterozygous for all three mutations. A single triple heterozygous BC₁F₁plant is backcrossed to Banket A1 to produce BC₂F₁ progeny. The processof backcrossing and identification of individuals heterozygous for allthree mutations is repeated through the BC₃F₁, BC₄F₁, BC₅F₁, BC₆F₁, andBC₇F₁ stages. At the BC₇F₁ stage, individuals heterozygous for all threemutations from each pedigree are self-pollinated to produce BC₇F₂ seed.A large number of BC₇F₂ progeny from each pedigree are genotyped toidentify individuals homozygous for all three mutations. A single BC₇F₂plant homozygous for all three mutations is self-pollinated to produce aBC₇F₃ family (Banket A1 SRC) in which the wild-type CYP82E4, CYP82E5v2,and CYP82E10 alleles of Banket A1 were replaced by the mutant alleles(cyp82e4 W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S).

The male-sterile (CMS) version of Banket A1 SRC (CMS Banket A1 SRC) isproduced by crossing a plant of CMS Banket A1 as a female with pollen ofBanket A1 SRC to produce male-sterile plants heterozygous for all threemutations. A single male-sterile plant resulting from this cross is thensubsequently backcrossed as a female to Banket A1 SRC to produce progenythat are segregating for individuals homozygous for all three mutations.Triple homozygous individuals are identified by DNA genotyping toproduce the CMS Banket A1 SRC line. Because the line is male-sterile, itis maintained via pollination with Banket A1 SRC.

Commercial Banket A1 SRC is produced by pollinating plants of CMS BanketA1 SRC with pollen of Banket A1 SRC.

Example 2 Testing of Banket A1 SRC

Banket A1 SRC is evaluated for cured leaf chemistry, yield, and physicalquality at three North Carolina field research locations during 2013(Reidsville and Waynesville). Banket A1 is included for comparison. Theexperimental design at each location is a randomized complete blockdesign with four replications. Experimental units are single 20-plantplots. Plots are harvested and air-cured. Plot weights are used todetermine per acre yields. Cured leaf is evaluated by a former USDAtobacco grader. Fifty gram composite leaf samples are collected fromeach plot and analyzed for percent nicotine, nornicotine, anatabine,anabasine, and percent nicotine conversion using gas chromatographyequipment.

Comparisons using the least significant difference (LSD) test indicatesthat Banket A1 SRC has significantly (P<0.05) lower levels ofnornicotine and percent nicotine conversion relative to Banket A1 (Table1). Banket A1 and Banket A1 SRC are not significantly different fromeach other for percent nicotine, yield, percent anabasine, percentanatabine, or percent total alkaloids.

Example 3 Breeding of Homozygous cyp82e4 W329Stop, the cyp82e5v2W422Stop, and cyp82e10 P381S Mutant Plants into the NC 2000 BurleyTobacco Cultivar

NC 2000 SRC is a backcross-derived version of burley tobacco cultivar NC2000 carrying introduced mutations in three genes (CYP82E4 (SEQ ID NO:5), CYP82E5 (SEQ ID NO: 8), and CYP82E10 (SEQ ID NO: 9)) previouslydocumented to encode for nicotine demethylase enzymes (Lewis et al.,Phytochemistry, 71 (2010), 1988-1998). The introduced mutations inCYP82E4 (cyp82e4 W329Stop (SEQ ID NO: 1)) and CYP82E5 (cyp82e5v2W422Stop (SEQ ID NO: 2)) encode for premature stop codons which renderthe genes non-functional. The introduced mutation in CYP82E10 (cyp82e10P381 S (SEQ ID NO: 11)) does not encode for a premature stop codon, butdoes render the gene product non-functional for converting nicotine tonornicotine (Id.). When in homozygous condition, the three mutationsresult in tobacco plants with (1) reduced genetic capacity todemethylate nicotine to form nornicotine, and (2) a correspondingdiminished potential to accumulate N-nitrosonornicotine (NNN), a potentcarcinogen found in many tobacco products. The mutation in CYP82E4(cyp82e4 W329Stop (SEQ ID NO: 1)) also provides phenotypic stability forthe “nicotine conversion” trait and eliminates the requirement toutilize the cumbersome “LC” method for reducing levels of nornicotine intobacco cultivars (see e.g., Jack et al. 2007. Implications of reducingnornicotine accumulation in burley tobacco: appendix A—the LC protocol.Rec. Adv. Tob. Sci. 33: 58-79).

The original tobacco cultivar NC 2000 is a fertile inbred line. CMS NC2000 is a cytoplasmic male-sterile version of NC 2000. To develop NC2000 SRC, an individual plant of NC 2000 is pollinated with a plant ofthe genetic background DH98-325-6 carrying mutations in each of thethree nicotine demethylase genes. F₁ individuals originating from thiscross and heterozygous for each mutation are backcrossed to therecurrent parent, NC 2000, to produce BC₁F₁ progeny. BC₁F₁ progeny arescreened using genotyping methodologies to identify individualsheterozygous for all three mutations. A single triple heterozygous BC₁F₁plant is backcrossed to NC 2000 to produce BC₂F₁ progeny. The process ofbackcrossing and identification of individuals heterozygous for allthree mutations is repeated through the BC₃F₁, BC₄F₁, BC₅F₁, BC₆F₁, andBC₇F₁ stages. At the BC₇F₁ stage, individuals heterozygous for all threemutations from each pedigree are self-pollinated to produce BC₇F₂ seed.A large number of BC₇F₂ progeny from each pedigree are genotyped toidentify individuals homozygous for all three mutations. A single BC₇F₂plant homozygous for all three mutations is self-pollinated to produce aBC₇F₃ family (NC 2000 SRC) in which the wild-type CYP82E4, CYP82E5v2,and CYP82E10 alleles of NC 2000 were replaced by the mutant alleles(cyp82e4 W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S).

The male-sterile (CMS) version of NC 2000 SRC (CMS NC 2000 SRC) isproduced by crossing a plant of CMS NC 2000 as a female with pollen ofNC 2000 SRC to produce male-sterile plants heterozygous for all threemutations. A single male-sterile plant resulting from this cross is thensubsequently backcrossed as a female to NC 2000 SRC to produce progenythat are segregating for individuals homozygous for all three mutations.Triple homozygous individuals are identified by DNA genotyping toproduce the CMS NC 2000 SRC line. Because the line is male-sterile, itis maintained via pollination with NC 2000 SRC.

Commercial NC 2000 SRC is produced by pollinating plants of CMS NC 2000SRC with pollen of NC 2000 SRC.

Example 4 Testing of NC 2000 SRC

NC 2000 SRC is evaluated for cured leaf chemistry, yield, and physicalquality at two North Carolina field research locations during 2013(Reidsville and Waynesville). NC 2000 is included for comparison. Theexperimental design at each location is a randomized complete blockdesign with four replications. Experimental units are single 20-plantplots. Plots are harvested and air-cured. Plot weights are used todetermine per acre yields. Cured leaf is evaluated by a former USDAtobacco grader. Fifty gram composite leaf samples are collected fromeach plot and analyzed for percent nicotine, nornicotine, anatabine,anabasine, and percent nicotine conversion using gas chromatographyequipment.

Comparisons using the least significant difference (LSD) test indicatesthat NC 2000 SRC has significantly (P<0.05) lower levels of nornicotineand percent nicotine conversion relative to NC 2000 (Table 2). NC 2000and NC 2000 SRC are not significantly different from each other forpercent nicotine, yield, percent anabasine, percent anatabine, orpercent total alkaloids.

Example 5 Breeding of Homozygous cyp82e4 W329Stop, the cyp82e5v2W422Stop, and cyp82e10 P381S Mutant Plants into the NC 2002LC BurleyTobacco Cultivar

NC 2002 SRC is a backcross-derived version of burley tobacco cultivar NC2002LC carrying introduced mutations in three genes (CYP82E4 (SEQ ID NO:5), CYP82E5 (SEQ ID NO: 8), and CYP82E10 (SEQ ID NO: 9)) previouslydocumented to encode for nicotine demethylase enzymes (Lewis et al.,Phytochemistry, 71 (2010), 1988-1998). The introduced mutations inCYP82E4 (cyp82e4 W329Stop (SEQ ID NO: 1)) and CYP82E5 (cyp82e5v2W422Stop (SEQ ID NO: 2)) encode for premature stop codons which renderthe genes non-functional. The introduced mutation in CYP82E10 (cyp82e10P381S (SEQ ID NO: 11)) does not encode for a premature stop codon, butdoes render the gene product non-functional for converting nicotine tonornicotine (Id.). When in homozygous condition, the three mutationsresult in tobacco plants with (1) reduced genetic capacity todemethylate nicotine to form nornicotine, and (2) a correspondingdiminished potential to accumulate N-nitrosonornicotine (NNN), a potentcarcinogen found in many tobacco products. The mutation in CYP82E4(cyp82e4 W329Stop (SEQ ID NO: 1)) also provides phenotypic stability forthe “nicotine conversion” trait and eliminates the requirement toutilize the cumbersome “LC” method for reducing levels of nornicotine intobacco cultivars (see e.g., Jack et al. 2007. Implications of reducingnornicotine accumulation in burley tobacco: appendix A—the LC protocol.Rec. Adv. Tob. Sci. 33: 58-79).

The original tobacco cultivar NC 2002LC is a fertile inbred line. CMS NC2002LC is a cytoplasmic male-sterile version of NC 2002LC. To develop NC2002 SRC, an individual plant of NC 2002LC is pollinated with a plant ofthe genetic background DH98-325-6 carrying mutations in each of thethree nicotine demethylase genes. F₁ individuals originating from thiscross and heterozygous for each mutation are backcrossed to therecurrent parent, NC 2002LC, to produce BC₁F₁ progeny. BC₁F₁ progeny arescreened using genotyping methodologies to identify individualsheterozygous for all three mutations. A single triple heterozygous BC₁F₁plant is backcrossed to NC 2002LC to produce BC₂F₁ progeny. The processof backcrossing and identification of individuals heterozygous for allthree mutations is repeated through the BC₃F₁, BC₄F₁, BC₅F₁, BC₆F₁, andBC₇F₁ stages. At the BC₇F₁ stage, individuals heterozygous for all threemutations from each pedigree are self-pollinated to produce BC₇F₂ seed.A large number of BC₇F₂ progeny from each pedigree are genotyped toidentify individuals homozygous for all three mutations. A single BC₇F₂plant homozygous for all three mutations is self-pollinated to produce aBC₇F₃ family (NC 2002 SRC) in which the wild-type CYP82E4, CYP82E5v2,and CYP82E10 alleles of NC 2002LC were replaced by the mutant alleles(cyp82e4 W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S).

The male-sterile (CMS) version of NC 2002 SRC (CMS NC 2002 SRC) isproduced by crossing a plant of CMS NC 2002LC as a female with pollen ofNC 2002 SRC to produce male-sterile plants heterozygous for all threemutations. A single male-sterile plant resulting from this cross is thensubsequently backcrossed as a female to NC 2002 SRC to produce progenythat are segregating for individuals homozygous for all three mutations.Triple homozygous individuals are identified by DNA genotyping toproduce the CMS NC 2002 SRC line. Because the line is male-sterile, itis maintained via pollination with NC 2002 SRC.

Commercial NC 2002 SRC is produced by pollinating plants of CMS NC 2002SRC with pollen of NC 2002 SRC.

Example 6 Testing of NC 2002 SRC

NC 2002 SRC is evaluated for cured leaf chemistry, yield, and physicalquality at two North Carolina field research locations during 2013(Reidsville and Waynesville). NC 2002LC is included for comparison. Theexperimental design at each location is a randomized complete blockdesign with four replications. Experimental units are single 20-plantplots. Plots are harvested and air-cured. Plot weights are used todetermine per acre yields. Cured leaf is evaluated by a former USDAtobacco grader. Fifty gram composite leaf samples are collected fromeach plot and analyzed for percent nicotine, nornicotine, anatabine,anabasine, and percent nicotine conversion using gas chromatographyequipment.

Comparisons using the least significant difference (LSD) test indicatesthat NC 2002 SRC has significantly (P<0.05) lower levels of nornicotineand percent nicotine conversion relative to NC 2002LC (Table 2). NC2002LC and NC 2002 SRC are not significantly different from each otherfor percent nicotine, yield, percent anabasine, percent anatabine, orpercent total alkaloids.

Example 7 Breeding of Homozygous cyp82e4 W329Stop, the cyp82e5v2W422Stop, and cyp82e10 P381S Mutant Plants into the Burley 21 SRC BurleyTobacco Cultivar

Burley 21 SRC is a backcross-derived version of burley tobacco cultivarBurley 21 SRC carrying introduced mutations in three genes (CYP82E4 (SEQID NO: 5), CYP82E5 (SEQ ID NO: 8), and CYP82E10 (SEQ ID NO: 9))previously documented to encode for nicotine demethylase enzymes (Lewiset al., Phytochemistry, 71 (2010), 1988-1998). The introduced mutationsin CYP82E4 (cyp82e4 W329Stop (SEQ ID NO: 1)) and CYP82E5 (cyp82e5v2W422Stop (SEQ ID NO: 2)) encode for premature stop codons which renderthe genes non-functional. The introduced mutation in CYP82E10 (cyp82e10P381S (SEQ ID NO: 11)) does not encode for a premature stop codon, butdoes render the gene product non-functional for converting nicotine tonornicotine (Id.). When in homozygous condition, the three mutationsresult in tobacco plants with (1) reduced genetic capacity todemethylate nicotine to form nornicotine, and (2) a correspondingdiminished potential to accumulate N-nitrosonornicotine (NNN), a potentcarcinogen found in many tobacco products. The mutation in CYP82E4(cyp82e4 W329Stop (SEQ ID NO: 1)) also provides phenotypic stability forthe “nicotine conversion” trait and eliminates the requirement toutilize the cumbersome “LC” method for reducing levels of nornicotine intobacco cultivars (see e.g., Jack et al. 2007. Implications of reducingnornicotine accumulation in burley tobacco: appendix A—the LC protocol.Rec. Adv. Tob. Sci. 33: 58-79).

The original tobacco cultivar Burley 21 SRC is a fertile inbred line.CMS Burley 21 is a cytoplasmic male-sterile version of Burley 21 SRC. Todevelop Burley 21 SRC, an individual plant of Burley 21 SRC ispollinated with a plant of the genetic background DH98-325-6 carryingmutations in each of the three nicotine demethylase genes. F₁individuals originating from this cross and heterozygous for eachmutation are backcrossed to the recurrent parent, Burley 21 SRC, toproduce BC₁F₁ progeny. BC₁F₁ progeny are screened using genotypingmethodologies to identify individuals heterozygous for all threemutations. A single triple heterozygous BC₁F₁ plant is backcrossed toBurley 21 SRC to produce BC₂F₁ progeny. The process of backcrossing andidentification of individuals heterozygous for all three mutations isrepeated through the BC₃F₁, BC₄F₁, BC₅F₁, BC₆F₁, and BC₇F₁ stages. Atthe BC₇F₁ stage, individuals heterozygous for all three mutations fromeach pedigree are self-pollinated to produce BC₇F₂ seed. A large numberof BC₇F₂ progeny from each pedigree are genotyped to identifyindividuals homozygous for all three mutations. A single BC₇F₂ planthomozygous for all three mutations is self-pollinated to produce a BC₇F₃family (Burley 21 SRC) in which the wild-type CYP82E4, CYP82E5v2, andCYP82E10 alleles of Burley 21 SRC were replaced by the mutant alleles(cyp82e4 W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S).

The male-sterile (CMS) version of Burley 21 SRC (CMS Burley 21 SRC) isproduced by crossing a plant of CMS Burley 21 as a female with pollen ofBurley 21 SRC to produce male-sterile plants heterozygous for all threemutations. A single male-sterile plant resulting from this cross is thensubsequently backcrossed as a female to Burley 21 SRC to produce progenythat are segregating for individuals homozygous for all three mutations.Triple homozygous individuals are identified by DNA genotyping toproduce the CMS Burley 21 SRC line. Because the line is male-sterile, itis maintained via pollination with Burley 21 SRC.

Commercial Burley 21 SRC is produced by pollinating plants of CMS Burley21 SRC with pollen of Burley 21 SRC.

Example 8 Testing of Burley 21 SRC

Burley 21 SRC is evaluated for cured leaf chemistry, yield, and physicalquality at two North Carolina field research locations during 2013(Reidsville and Waynesville). Burley 21 SRC is included for comparison.The experimental design at each location is a randomized complete blockdesign with four replications. Experimental units are single 20-plantplots. Plots are harvested and air-cured. Plot weights are used todetermine per acre yields. Cured leaf is evaluated by a former USDAtobacco grader. Fifty gram composite leaf samples are collected fromeach plot and analyzed for percent nicotine, nornicotine, anatabine,anabasine, and percent nicotine conversion using gas chromatographyequipment.

Comparisons using the least significant difference (LSD) test indicatesthat Burley 21 SRC has significantly (P<0.05) lower levels ofnornicotine and percent nicotine conversion relative to Burley 21 SRC(Table 1). Burley 21 SRC and Burley 21 SRC are not significantlydifferent from each other for percent nicotine, yield, percentanabasine, percent anatabine, or percent total alkaloids.

TABLE 1 Entry means for experiment EX14-157 entries evaluated in two2013 North Carolina environments (Reidsville and Waynesville). TotalNicotine Yield Grade Nicotine Nornicotine Anabasine Anatabine AlkaloidsConversion Variety (lbs/A) Index (%) (%) (%) (%) (%) (%) Banket A1 207172.4 2.757 0.049 0.017 0.258 3.081 1.796 Banket A1 SRC 1948 72.2 3.2140.018* 0.019 0.284 3.535 0.594* Burley 21 1985 72.7 2.250 0.060 0.0120.174 2.495 2.649 Burley 21 SRC 1897 74.7 2.881 0.018* 0.014 0.218 3.1300.631* NC 3LC 2168 73.9 1.921 0.117 0.012 0.180 2.230 5.523 NC 3 SRC2097 75.9 2.154 0.011* 0.011 0.175 2.351 0.504* NC 4 SRC 2143 74.6 2.3950.013* 0.012 0.217 2.637 0.554* NC 6LC 2275 75.7 2.093 0.221 0.012 0.2042.530 8.965 NC 6 SRC 2245 72.1 2.380 0.010* 0.012 0.199 2.601 0.431*DH98-325-5 2187 72.1 2.066 0.045 0.012 0.200 2.323 2.205 DH98-325-5 SRC2086 72.5 2.332 0.013* 0.012 0.205 2.562 0.533* DH19 1898 70.7 1.8540.044 0.011 0.158 2.066 2.257 DH19 SRC 2242 72.5 1.870 0.018* 0.0120.159 2.058 0.998* NC1426-17 1924 71.8 1.802 0.046 0.012 0.211 2.0722.565 NC1426-17 SRC 2056 75.2 2.030 0.012* 0.012 0.214 2.269 0.614*NC1426-11 2415 71.2 2.228 0.078 0.013 0.232 2.551 3.513 NC1426-11 SRC 2056* 75.4* 2.121 0.011* 0.011 0.181* 2.324 0.524* VA 509LC 1975 72.92.197 0.169 0.013 0.187 2.567 7.107 Ky 14 LC 2237 74.2 2.151 0.072 0.0120.209 2.444 3.407 Banket A1 is a fertile burley inbred variety. BanketA1 SRC is a backcross-derived version of Banket A1 carrying introducedmutations in three genes (cyp82e4 W329Stop, cyp82e5v2 W422Stop, andcyp82e10 P381S). Burley 21 is a fertile burley inbred variety. Burley 21SRC is a backcross-derived version of Burley 21 carrying introducedmutations in three genes (cyp82e4 W329Stop, cyp82e5v2 W422Stop, andcyp82e10 P381S). Tobacco cultivar NC 3LC is a hybrid generated bypollinating plants of a male-sterile breeding line CMS NC1209-23 withpollen produced by fertile breeding line DH19. Tobacco cultivar NC 3 SRCis a hybrid generated by pollinating plants of a male-sterile breedingline CMS NC1209-23 SRC with pollen produced by fertile breeding lineDH19 SRC. Tobacco cultivar NC 4 SRC is a hybrid generated by pollinatingplants of a male-sterile breeding line CMS NC1426-11 SRC with pollenproduced by fertile breeding line NC1426-17 SRC. Tobacco cultivar NC 6LCis a hybrid generated by pollinating plants of a male-sterile breedingline CMS NC1209-23 with pollen produced by fertile breeding lineDH98-325-5. Tobacco cultivar NC 6 SRC is a hybrid generated bypollinating plants of a male-sterile breeding line CMS NC1209-23 SRCwith pollen produced by fertile breeding line DH98-325-5 SRC. DH98-325-5is a fertile burley inbred variety. DH98-325-5 SRC is abackcross-derived version of DH98-325-5 carrying introduced mutations inthree genes (cyp82e4 W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S).DH 19 is a fertile burley inbred variety. DH 19 SRC is abackcross-derived version of DH 19 carrying introduced mutations inthree genes (cyp82e4 W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S).NC1426-17 is a fertile burley inbred variety. NC1426-17 SRC is abackcross-derived version of NC1426-17 carrying introduced mutations inthree genes (cyp82e4 W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S).NC1426-11 is a fertile burley inbred variety. NC1426-11 SRC is abackcross-derived version of NC1426-11 carrying introduced mutations inthree genes (cyp82e4 W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S).KY 14 LC and VA 509 LC are LC-selected burley tobacco varieties KY 14and VA 509. *Indicates significantly different (P < 0.05) from thenearly isogenic LC variety or regular breeding line. Significance testswere based upon log transformed valued for % nicotine, % nornicotine, %anabasine, % anatabine, and % nicotine conversion. Significance testswere based upon non-transformed values for the remainder of the measuredcharacteristics.

TABLE 2 Entry means for experiment EX13-158 entries evaluated in two2013 North Carolina environments (Reidsville and Waynesville). TotalNicotine Yield Grade Nicotine Nornicotine Anabasine Anatabine AlkaloidsConversion Variety (lbs/A) Index (%) (%) (%) (%) (%) (%) NC 174 176965.2 2.180 0.061 0.012 0.167 2.420 2.689 NC 174 SRC 1489 67.7 1.606*0.013* 0.010 0.117* 1.746 0.826* NC821-11 1671 66.6 1.708 0.045 0.0120.241 2.005 2.658 NC821-11 SRC 1588 66.5 1.535 0.013* 0.010 0.170* 1.7280.803* NC 2000LC 1750 71.5 2.651 0.076 0.015 0.230 2.973 2.834 NC 2000SRC 1652 68.9 2.914 0.021* 0.017 0.238 3.191 0.705* NC 2002LC 1722 71.92.143 0.091 0.014 0.191 2.439 4.280 NC 2002 SRC 1596 69.0 1.856 0.015*0.009* 0.127* 2.007 0.788* NC 5LC 1769 65.7 1.575 0.123 0.011 0.1701.879 7.363 NC 5 SRC 1897 66.5 1.701 0.014* 0.010 0.155 1.880 0.781* NC645 1919 72.9 2.134 0.078 0.012 0.177 2.402 3.226 NC645 BmrBmr SRC 174872.1 2.717 0.024* 0.014 0.225 2.980 0.892* NC 775 1741 69.2 1.938 0.1230.012 0.151 2.224 6.004 NC 775 BmrBmr SRC 1805 69.3 2.008 0.014* 0.0110.156 2.189 0.716* NC 8 SRC 1845 69.3 2.432 0.020* 0.013 0.191 2.6570.857* Ky 14LC 1847 68.0 1.833 0.043 0.012 0.156 2.044 2.216 VA 509LC1756 69.8 2.145 0.079 0.013 0.167 2.404 3.642 NC 174 is a fertile burleyinbred variety. NC 174 SRC is a backcross-derived version of NC 174carrying introduced mutations in three genes (cyp82e4 W329Stop,cyp82e5v2 W422Stop, and cyp82e10 P381S). NC821-11 is a fertile burleyinbred variety. NC821-11 SRC is a backcross-derived version of NC821-11carrying introduced mutations in three genes (cyp82e4 W329Stop,cyp82e5v2 W422Stop, and cyp82e10 P381S). NC 2000LC is a LC-selectedfertile burley inbred variety NC 2000. NC 2000 SRC is abackcross-derived version of NC 2000 carrying introduced mutations inthree genes (cyp82e4 W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S).NC 2002LC is a LC-selected fertile burley inbred variety NC 2002. NC2002 SRC is a backcross-derived version of NC 2002 carrying introducedmutations in three genes (cyp82e4 W329Stop, cyp82e5v2 W422Stop, andcyp82e10 P381S). Tobacco cultivar NC 5LC is a hybrid generated bypollinating plants of a male-sterile breeding line CMS NC174 with pollenproduced by fertile breeding line NC821-11. Tobacco cultivar NC 5 SRC isa hybrid generated by pollinating plants of a male-sterile breeding lineCMS NC174 SRC with pollen produced by fertile breeding line NC821-11SRC. NC 645 is a fertile burley inbred variety. NC645 BmrBmr SRC is abackcross-derived version of NC645 carrying introduced mutations inthree genes (cyp82e4 W329Stop, cyp82e5v2 W422Stop, and cyp82e10 P381S)and homozygous for an introduced major blue mold resistance genedesignated as Bmr. NC 775 is a fertile burley inbred variety. NC775BmrBmr SRC is a backcross-derived version of NC775 carrying introducedmutations in three genes (cyp82e4 W329Stop, cyp82e5v2 W422Stop, andcyp82e10 P381S) and homozygous for an introduced major blue moldresistance gene designated as Bmr. Hybrid cultivar NC 8 SRC is producedby pollinating plants of CMS NC775 Bmr/Bmr SRC with pollen of NC645Bmr/Bmr SRC. KY 14LC and VA 509LC are LC-selected burley tobaccovarieties KY 14 and VA 509, respectively. *Indicates significantlydifferent (P < 0.05) from the nearly isogenic LC variety or regularbreeding line. Significance tests are based upon log transformed valuedfor % nicotine, % nornicotine, % anabasine, % anatabine, and % nicotineconversion. Significance tests are based upon non-transformed values forthe remainder of the measured characteristics.

DEPOSIT INFORMATION

A deposit of the proprietary inbred plant lines disclosed above andrecited in the appended claims have been made with American Type CultureCollection (ATCC), 10801 University Boulevard, Manassas, Va. 20110. Thedate of deposit for Banket A1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMSNC 2000 SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley 21 SRC, and CMS Burley21 SRC was Feb. 26, 2014. The deposits of 2500 seeds for each varietywas taken from the same deposits maintained since prior to the filingdate of this application. Upon issuance of a patent, all restrictionsupon the deposits will be removed, and the deposits are intended to meetall of the requirements of 37 C.F.R. §1.801-1.809. The ATCC has issuedthe following accession numbers: PTA-121043 for Banket A1 SRC,PTA-121054 for CMS Banket A1 SRC, PTA-121053 for NC 2000 SRC, PTA-121045for CMS NC 2000 SRC, PTA-121058 for NC 2002 SRC, PTA-121041 for CMS NC2002 SRC, PTA-121059 for Burley 21 SRC, and PTA-121044 for CMS Burley 21SRC. These deposits will be maintained in the depository for a period of30 years, or 5 years after the last request, or for the effective lifeof the patent, whichever is longer, and will be replaced as necessaryduring that period. Applicants do not waive any infringement of theirrights granted under this patent or under the Plant Variety ProtectionAct (7 U.S.C. 2321 et seq.).

What is claimed is:
 1. Cured tobacco or a tobacco product preparedtherefrom, wherein said cured tobacco is from a tobacco plant of acultivar or a hybrid produced from said cultivar, wherein said cultivaris selected from the group consisting of Banket A1 SRC, CMS Banket A1SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley21 SRC, and CMS Burley 21 SRC; and wherein representative sample seedsof said Banket A1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC,NC 2002 SRC, CMS NC 2002 SRC, Burley 21 SRC, and CMS Burley 21 SRC havebeen deposited at the ATCC with the following ATCC Accession Nos.:PTA-121043 for Banket A1 SRC, PTA-121054 for CMS Banket A1 SRC,PTA-121053 for NC 2000 SRC, PTA-121045 for CMS NC 2000 SRC, PTA-121058for NC 2002 SRC, PTA-121041 for CMS NC 2002 SRC, PTA-121059 for Burley21 SRC, and PTA-121044 for CMS Burley 21 SRC.
 2. The tobacco product ofclaim 1, wherein said tobacco product is selected from the groupconsisting of a tobacco blend, a pipe tobacco, a cigar tobacco, acigarette tobacco, a chewing tobacco, a leaf tobacco, a shreddedtobacco, and a cut tobacco.
 3. The tobacco product of claim 1, whereinsaid tobacco product is selected from the group consisting of acigarillo, a non-ventilated recess filter cigarette, a vented recessfilter cigarette, a cigar, snuff, and a chewing tobacco.
 4. The tobaccoproduct of claim 3, wherein said tobacco product has an amount ofnornicotine of less than about 3 mg/g.
 5. The tobacco product of claim4, wherein said amount of nicotine is selected from the group consistingof 3.0 mg/g, 2.5 mg/g, 2.0 mg/g, 1.5 mg/g, 1.0 mg/g, 750 μg/g, 500 pg/g,250 pg/g, 100 pg/g, 75 pg/g, 50 pg/g, 25 pg/g, 10 pg/g, 7.0 pg/g, 5.0pg/g, 4.0 pg/g, 1.0 pg/g, 1.0 pg/g, 0.5 pg/g, 0.4 pg/g, 0.2 pg/g, 0.1pg/g, 0.05 pg/g, 0.01 pg/g, and undetectable.
 6. The tobacco product ofclaim 3, wherein said tobacco product has an amount ofN′-nitrosonornicotine (NNN) of less than about 10 pg/g.
 7. The tobaccoproduct of claim 6, wherein said amount of NNN is selected from thegroup consisting of 10 pg/g, 7.0 pg/g, 5.0 pg/g, 4.0 pg/g, 2.0 pg/g, 1.0pg/g, 0.5 pg/g, 0.4 pg/g, 0.2 pg/g, 0.1 pg/g, 0.05 pg/g, 0.01 pg/g, andundetectable.
 8. The tobacco product of claim 1, wherein said curedtobacco is at least about 5% by dry weight of the total tobacco in saidtobacco product.
 9. The tobacco product of claim 8, wherein said curedtobacco has a dry weight percentage of said total cured tobacco selectedfrom the group consisting of at least about 10%, at least about 20%, atleast about 30%, at least about 40%, at least about 50%, at least about60%, at least about 70%, at least about 80%, at least about 90%, and atleast about 95%.
 10. A method of producing a tobacco product,comprising: a. providing cured tobacco from a tobacco plant of acultivar or a hybrid produced from said cultivar, wherein said cultivaris selected from the group consisting of Banket A1 SRC, CMS Banket A1SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley21 SRC, and CMS Burley 21 SRC; and b. preparing a tobacco product fromsaid cured tobacco, wherein representative sample seeds of said BanketA1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002 SRC,CMS NC 2002 SRC, Burley 21 SRC, and CMS Burley 21 SRC have beendeposited at the ATCC with the following ATCC Accession Nos.: PTA-121043for Banket A1 SRC, PTA-121054 for CMS Banket A1 SRC, PTA-121053 for NC2000 SRC, PTA-121045 for CMS NC 2000 SRC, PTA-121058 for NC 2002 SRC,PTA-121041 for CMS NC 2002 SRC, PTA-121059 for Burley 21 SRC, andPTA-121044 for CMS Burley 21 SRC.
 11. A seed of tobacco cultivarselected from the group consisting of Banket A1 SRC, CMS Banket A1 SRC,NC 2000 SRC, CMS NC 2000 SRC, NC 2002 SRC, CMS NC 2002 SRC, Burley 21SRC, and CMS Burley 21 SRC; wherein representative sample seeds of saidBanket A1 SRC, CMS Banket A1 SRC, NC 2000 SRC, CMS NC 2000 SRC, NC 2002SRC, CMS NC 2002 SRC, Burley 21 SRC, and CMS Burley 21 SRC have beendeposited at the ATCC with the following ATCC Accession Nos.: PTA-121043for Banket A1 SRC, PTA-121054 for CMS Banket A1 SRC, PTA-121053 for NC2000 SRC, PTA-121045 for CMS NC 2000 SRC, PTA-121058 for NC 2002 SRC,PTA-121041 for CMS NC 2002 SRC, PTA-121059 for Burley 21 SRC, andPTA-121044 for CMS Burley 21 SRC.
 12. A tobacco plant, or part thereof,produced from said seed of claim
 11. 13. The part of the plant of claim12, wherein said part is selected from the group consisting of aharvested leaf, a pollen, an ovule, an embryo, a cotyledon, a hypocotyl,a meristematic cell, a protoplast, a root, a root tip, a pistil, ananther, a flower, a shoot, a stem, a pod, a petiole, and combinationsthereof.
 14. A harvested leaf of said tobacco plant of claim
 12. 15. Theharvested leaf of claim 14, wherein said leaf has a reduced amount ofnornicotine and/or N′-nitrosonornicotine (NNN).
 16. The harvested leafof claim 15, wherein said reduced amount of nornicotine and/orN-nitrosonomicotine (NNN) is reduced in a smoke stream produced fromsaid leaf.